We narrowed to 31,119 results for: PLE

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pET28a_6HT-TBP6.7(R49A)

Plasmid

#112726

Purpose

Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R49A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R49A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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ArpC5_pLib

Plasmid

#173682

Purpose

ArpC5 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC5 (ARPC5 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1983

Plasmid

#49114

Purpose

AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple253-iCre

Use

AAV

Expression

Mammalian

Promoter

PITX3

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5FRTTO-NHASt-codoptUL50

Plasmid

#135101

Purpose

Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL50 codon optimized

Tags

HA-Strep-Strep

Expression

Mammalian

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS2049

Plasmid

#79657

Purpose

ssAAV genome with Ple260 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple260-EmGFP WPRE

Use

AAV

Promoter

PAX6 Retinal Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2045

Plasmid

#79653

Purpose

ssAAV genome with Ple256 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple256-EmGFP WPRE

Use

AAV

Promoter

PAX6 enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS2048

Plasmid

#79656

Purpose

ssAAV genome with Ple259 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple259-EmGFP WPRE

Use

AAV

Promoter

PAX6 MiniPromoter

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-Plxnb2-ISH-2kb

Plasmid

#68036

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B2 (Plxnb2 Mouse)

Use

Mrna in situ hybridization probe

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEMS2043

Plasmid

#79651

Purpose

ssAAV genome with Ple254 (PAX6 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

Depositor

Elizabeth Simpson

Article

Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

Insert

ssAAV Ple254-EmGFP WPRE

Use

AAV

Promoter

PAX6 Retinal Enhancer

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pEMS1984

Plasmid

#49115

Purpose

AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple253-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

PITX3

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5FRTTO-NHASt-codoptUL23

Plasmid

#135104

Purpose

Codon-optimized HSV-1 UL23 with N-terminal HAStrep tag

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Insert

HSV-1 UL23 codon optimized

Tags

HA-Strep-Strep (N terminal on backbone)

Expression

Mammalian

Available Since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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Plxna3-AP-His

Plasmid

#71998

Purpose

Expresses the extracellular region of the PlexinA3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna3 (Plxna3 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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ArpC2_pLib

Plasmid

#173679

Purpose

ArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC2 (ARPC2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1958

Plasmid

#82569

Purpose

High copy number plasmid containing PR2/3:Con MiniPromoter insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

PRS2/3:Con

Use

Pleiades promoter project [sic, pleaides plieades]

Available Since

Sept. 14, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1978

Plasmid

#49110

Purpose

AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple251-icre-WPRE

Use

AAV

Expression

Mammalian

Promoter

C8ORF46

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2026

Plasmid

#49133

Purpose

AAV plasmid with TNNT1-B (Ple301) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple301-iCre

Use

AAV

Expression

Mammalian

Promoter

TNNT1-B

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pRS406-PHO5-GFP-CERT PH domain EE mutation

Plasmid

#58833

Purpose

Expresses GFP-Tagged and mutated CERT PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Arginine 43 to Glutamate

Promoter

PHO5

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-IFT38

Plasmid

#218723

Purpose

Expresses N-terminally EGFP-tagged IFT38 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT38 (CLUAP1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT25

Plasmid

#218721

Purpose

Expresses N-terminally EGFP-tagged IFT25 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

IFT25 (HSPB11 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Plasmid

#217509

Purpose

KD Recombinase dependent conditional 1X ALFA cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-1XALFA

Tags

ALFA tag

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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