162,300 results

1. pAAV.CAG.GCaMP6s.WPRE.SV40

   Plasmid

   #100844

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HA-SOCS1

   Plasmid

   #174571

   Purpose

   Human HA-tagged SOCS1 expression (N-term. tag) (CMV prom.)

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   HA-SOCS1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc

   Plasmid

   #193346

   Purpose

   tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc)

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Sox2-17 is engineered highly cooperative chimeric…

   Promoter

   TetO mini CMV

   Available since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCXLE-Sox2AV-T2A-Klf4-E2A-Myc

   Plasmid

   #210017

   Purpose

   episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset.

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   Sox2AV-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Sox2A61V is a point mutant of Sox2 that enhances …

   Promoter

   CAG

   Available since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pVP91A-pcaHG

   Plasmid

   #113766

   Purpose

   Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

   Depositor

   John Lipscomb

   Article

   Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

   Inserts

   β subunit of Protocatechuate 3,4- Dioxygenase
   α subunit of Protocatechuate 3,4- Dioxygenase
   

   Use

   Tags

   Met-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter

   Available since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB_tre_Cas9

   Plasmid

   #126029

   Purpose

   PiggyBac cargo vector expressing doxycycline inducible Cas9

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Inserts

   humanized S. pyrogenes Cas9
   Hygromycin resistance
   

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1 alpha and TRE

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

   Plasmid

   #191795

   Purpose

   vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13d
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   A134V, A140V, A141V, A143V

   Promoter

   EFS, hU6

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JNK-KTR-mStrawberry reporter

   Plasmid

   #158689

   Purpose

   This plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pimEJ5GFP

   Plasmid

   #44026

   Depositor

   Jeremy Stark

   Article

   Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

   Insert

   EJ5GFP egfp-based chromosomal break reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   pCAGGS promoter and eGFP separated by pgkPURO cas…

   Promoter

   pCAGGS

   Available since

   May 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lamp1-RFP

    Plasmid

    #1817

    Purpose

    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    lysosome associated membrane protein 1 (Lamp1 Rat)
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    D50E (not important for function of plasmid)

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge