We narrowed to 6,927 results for: 11

1. pRSF_his6_sumo_229E_NSP14

   Plasmid

   #213521

   Purpose

   Expresses NSP14 of hCoV 229E

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

   Insert

   hCoV 229E NSP14
   

   Use

   Tags

   His6-sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV5 n-myc mPirh2

   Plasmid

   #212994

   Purpose

   expresses mouse PIRH2

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   pirh2 (Rchy1 Mouse)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBOBI C-FLAG PHGDH T78A

   Plasmid

   #212997

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T78A

   Promoter

   cmv

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBOBI C-FLAG PHGDH R135W

   Plasmid

   #212998

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R135W

   Promoter

   cmv

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBOBI N-FLAG HIPK2

   Plasmid

   #213004

   Purpose

   expresses mouse HIPK2

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   HIPK2 (Hipk2 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBOBI C-FLAG PHGDH T57A

   Plasmid

   #212996

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T57A

   Promoter

   cmv

   Available since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pαH-rS2d

   Plasmid

   #183516

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike protein S-GSAS-rS2d with (682-685) furin side replaced with GSAS and mutation S383C, D985C

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6

   Insert

   rS2d (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. RALA V25L

   Plasmid

   #122927

   Purpose

   Bacterial expression of RALA V25L

   Depositor

   Greg Cooper

   Article

   Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

   Insert

   RALA (RALA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   V25L

   Promoter

   T7

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. RALA V25M

   Plasmid

   #122928

   Purpose

   Bacterial expression of RALA V25M

   Depositor

   Greg Cooper

   Article

   Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

   Insert

   RALA (RALA Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   V25M

   Promoter

   T7

   Available since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. RALA D130G

    Plasmid

    #122929

    Purpose

    Bacterial expression of RALA D130G

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    D130G

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. RALA S157A

    Plasmid

    #122930

    Purpose

    Bacterial expression of RALA S157A

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S157A

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. RALA R176X

    Plasmid

    #122931

    Purpose

    Bacterial expression of RALA R176X

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    R176X

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RALA WT

    Plasmid

    #122925

    Purpose

    Bacterial expression of RALA WT

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RALA G23D

    Plasmid

    #122926

    Purpose

    Bacterial expression of RALA G23D

    Depositor

    Greg Cooper

    Article

    Hiatt et al PLoS Genet. 2018 Nov 30;14(11):e1007671.

    Insert

    RALA (RALA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    G23D

    Promoter

    T7

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEAHISSOarD1

    Plasmid

    #97067

    Purpose

    Expressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    S. solfataricus Sso Ard1 protein
    

    Use

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Expression

    Mutation

    none

    Promoter

    T7

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEAHISSIRV418/gp25

    Plasmid

    #97052

    Purpose

    Expressing Sulfolobus islandicus rudivirus (SIRV) orf 418/gp25 protein

    Depositor

    Huanting Liu, James Naismith

    Article

    Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

    Insert

    SIRV orf 418/gp25
    

    Use

    Tags

    N-terminal TEV protease cleavable 6xHis tag

    Expression

    Bacterial

    Mutation

    none

    Promoter

    T7

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SypHer mt

    Plasmid

    #48251

    Purpose

    pH sensor, targeted to mitochondria

    Depositor

    Nicolas Demaurex

    Article

    Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

    Insert

    SypHer
    

    Use

    Tags

    2 mitochondrial targeting sequences

    Expression

    Mammalian

    Mutation

    C199S

    Promoter

    CMV

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pInt

    Plasmid

    #18941

    Depositor

    Michele Calos

    Article

    Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

    Insert

    phiC31 integrase (int Streptomyces phage phiC31)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    under lacZ promoter

    Promoter

    Available since

    Aug. 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Paul Blainey, Stephen Quake

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLVX-PERK(LD)-EGFP-HOTag3

    Plasmid

    #225678

    Purpose

    Used to generate stable cell lines that express PERK(LD)-EGFP-HOTag3 and to detect the unfolded proteins in the ER lumen.

    Depositor

    Likun Wang

    Article

    Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGTR

    Plasmid

    #63143

    Purpose

    PCR template to construct polycistronic tRNA-gRNA

    Depositor

    Yinong Yang

    Article

    Xie et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    gRNA-tRNA fusion
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. GFP-UtrCH

    Plasmid

    #26737

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261).…

    Promoter

    CMV IE94

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLVX-PERK(LD)-EGFP

    Plasmid

    #225680

    Purpose

    Used to generate stable cell lines that express PERK(LD)-EGFP. This plasmid serves as a control for pLVX-PERK(LD)-EGFP-HOTag3.

    Depositor

    Likun Wang

    Article

    Xu et al Cell Rep. 2024 Jun 25;43(6):114358. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAH01Sp::Cas9

    Plasmid

    #128160

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-postASAP

    Plasmid

    #178792

    Purpose

    Expresses voltage sensor targeted to dendrites and spines

    Depositor

    Rafael Yuste

    Article

    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

    Insert

    FingR.PSD95 and ASAP2f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutations in amino acids L146G, S147T N149R, S150…

    Promoter

    CAG

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RV-Cag-Dio-GFP

    Plasmid

    #87662

    Purpose

    retroviral vector encoding cre dependent expression of GFP

    Depositor

    Oscar Marin

    Article

    Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

    Insert

    eGFP
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PM-ExRai-PKNAR

    Plasmid

    #226633

    Purpose

    Plasma membrane-targeted ExRai-PKNAR

    Depositor

    Ju Chen

    Article

    Bogomolovas et al Commun Biol. 2025 Jan 22;8(1):109. doi:

    Insert

    PM-ExRai-PKNAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Plasma membrane targeting N-terminal 11 amino aci…

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AICSDP-117:POLR2A-mEGFP

    Plasmid

    #164500

    Purpose

    Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

    Depositor

    Allen Institute for Cell Science

    Insert

    POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pCAH01Sp::Cas9D10A

    Plasmid

    #128161

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9D10A variant

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    Ptet

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge