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YEplac181-TAF14

Plasmid

#8625

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

TAF14 (TAF14 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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EMX1_sgRNA

Plasmid

#100555

Purpose

Expresses EMX1 sgRNA. Target sequence: GAGTCCGAGCAGAAGAAGAA

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

EMX1 sgRNA

Promoter

U6

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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SMX DEST vector

Plasmid

#62399

Purpose

Empty Backbone

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Type

Empty backbone

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p177 Grg-1s EGFP

Plasmid

#11066

Depositor

Ramesh Shivdasani

Article

Lepourcelet et al J Biol Chem. 2002 Dec 6. 277(49):47732-4

Insert

Grg1-S (Tle1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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YEplac181-DST1

Plasmid

#8624

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

DST1 (DST1 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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FISSEQ-hgRNA-Design1

Plasmid

#100571

Purpose

FISSEQ-hgRNA-Design1 in a lentiviral backbone

Depositor

George Church

Article

Kalhor et al Nat Methods. 2017 Feb;14(2):195-200. doi

Insert

FISSEQ-hgRNA-Design1

Use

CRISPR, Lentiviral, and Synthetic Biology

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSB819-PKR-gib

Plasmid

#20031

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-titi

Plasmid

#20035

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
YEplac181-SUA7

Plasmid

#8627

Depositor

Jeff Strathern

Article

Malagon et al Genetics 2004 Mar;166(3):1215-27.

Insert

SUA7 (SUA7 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

Wild type

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only

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pSB305-K3L-var

Plasmid

#20055

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

K3L

Expression

Yeast

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only
pJJH1308

Plasmid

#36911

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Expression

Bacterial, Mammalian, and Y…

Available Since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pAG78 3'UTR

Plasmid

#12023

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N2 3'UTR

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pAG84 3'UTR

Plasmid

#12026

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N5 3'UTR

Use

Luciferase

Expression

Mammalian

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only

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pFredA-PTTG1

Plasmid

#16584

Depositor

Bert Vogelstein

Article

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

PTTG1 (PTTG1 Human)

Expression

Mammalian

Mutation

5' targeting element

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-ora-L394F

Plasmid

#20052

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R

Expression

Yeast

Mutation

L394F

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pLentiX1-CAG-TagBFP

Plasmid

#244166

Purpose

Lentiviral expression of TagBFP

Depositor

Kate Galloway

Article

Lende-Dorn et al Cell Rep. 2025 Sep 23;44(9):116226. doi:

Insert

TagBFP

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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NSK252 SFFV-mCherry-SGc(os13.NOT-SCN1A_20N.45,64,45)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247286

Purpose

modulADAR sensor for NOT SCN1a exon 20N

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-os13.NOT-SCN1A_20N.45,64,45-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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EK0716 SFFV-mCherry-SGc(s70587.os18)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247271

Purpose

modulADAR sensor against sythetic target transcript (35nt GluR-B stemloop)

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-s70587.os18-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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NSK162 CMV-TO-SMN2 Nluc - inactive (exon 6, 8)

Plasmid

#247275

Purpose

Expresses human SMN2 exons 6, 8 followed by inactive Nluc

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

SMN2 exons 6,8-Nluc (SMN2 Human)

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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EK0698 SFFV-mCherry-SGc(s70587.os13)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247270

Purpose

modulADAR sensor against sythetic target transcript (45ntGABRA3 stemloop)

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-s70587.os13-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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EK0718 SFFV-mCherry-SGc(s70587.os20)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247273

Purpose

modulADAR sensor against sythetic target transcript (36nt GABRA3 stem-loop)

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-s70587.os20-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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EK0717 SFFV-mCherry-SGc(s70587.os19)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247272

Purpose

modulADAR sensor against sythetic target transcript (41nt GluR-B stemloop)

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-s70587.os19-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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QBEn

Plasmid

#238002

Purpose

Expresses the QBEn base editor in mammalian cells

Depositor

Kevin Zhao

Article

Hu et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

QBEn base editor

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pHR_CMV_MeltEGFR-37-mCherry

Plasmid

#234065

Purpose

Encodes EGFR intracellular domain controlled by a Melt variant with a switching temperature of 37°C

Depositor

Lukasz Bugaj

Article

Benman et al Nat Methods. 2025 Jan 23. doi: 10.1038/s

Insert

MeltEGFR-37-mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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L1p1_min35S_Luciferase

Plasmid

#231035

Purpose

Cloning vector to clone promoter fragment in front of minimal p35S:Luciferase:tNOS

Depositor

Wilma van Esse

Article

Winkelmolen et al bioRxiv 2024.08.22.609225

Insert

Minimal p35S:Luciferase:tNOS

Use

Luciferase

Expression

Bacterial

Promoter

minimal p35S

Available Since

March 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGH1173

Plasmid

#223677

Purpose

pEF-HA-PYL1-NLS-CloningSite-mCherry-puroR

Depositor

Gaelen Hess

Article

Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgCTG-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222857

Purpose

This plasmid codes for the Slug-KH nickase with a sg(CTG)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgCTG

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-ITR2-H1-sgAGC-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222859

Purpose

This plasmid codes for the Slug-KH nickase with a sg(AGC)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgAGC

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-ITR2-H1-sgCAG-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222858

Purpose

This plasmid codes for the Slug-KH nickase with a sg(CAG)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgCAG

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-ITR2-H1-sgGCA-SlugD10A-3XFLAG-SV40 NLS-ITR2

Plasmid

#222860

Purpose

This plasmid codes for the Slug-KH nickase with a sg(GCA)6

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

Slug-KHD10A+sgGCA

Use

CRISPR

Tags

3X FLAG, SV40 NLS, ITR2 and ITR2

Expression

Mammalian

Mutation

KH variant and D10A mutation

Promoter

H1 and eCMV

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge