We narrowed to 22,748 results for: Sele

1. pMRX-IRES-puro-3xFLAG-NBR1 deltaUBA

   Plasmid

   #204547

   Purpose

   Expresses 3xFLAG-tagged NBR1 deltaUBA in mammalian cells

   Depositor

   Masaaki Komatsu

   Article

   Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

   Insert

   NBR1 (NBR1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   deltaUBA

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. his3MX6-ins3

   Plasmid

   #195040

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tCYC1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   His5+
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX6P2-MDMX (1-120)

   Plasmid

   #62065

   Purpose

   Expression of human MDM2 (1-120) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

   Insert

   MDM4 (MDM4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   contains residues 1-120

   Promoter

   tac

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. ADRB2-DuET

   Plasmid

   #213181

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRB2 (ADRB2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ADRA2A-DuET

   Plasmid

   #213177

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   ADRA2A (ADRA2A Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-DIO-GRAB_CCK1.0

   Plasmid

   #208674

   Purpose

   Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-Neo-NCOA7g1 (BB34)

   Plasmid

   #139452

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ284-v2

   Plasmid

   #138119

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   Mb2Cas12a v2
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   D172R, N563R and K569R

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-DIO-GRAB_CRF1.0

   Plasmid

   #208662

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-PE SpG

    Plasmid

    #179081

    Purpose

    All-in-one prime editor piggyBac transposon, SpG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

    Promoter

    CAG

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. attB-mCh-Dnmt3b6-Poly(A)-NeoR

    Plasmid

    #65554

    Purpose

    Plasmid for Bxb1-mediated recombination of the mCh-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    isoform 6

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1α-Flex/3'USS-hM4D/2A/GFP(ATG mut)

    Plasmid

    #197892

    Purpose

    Can be used to generate AAV virus that will express hM4D/2A/GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    hM4D/2A/GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    EF1a

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hGRM1-RFP

    Plasmid

    #22920

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLNHA-C1-HsGIGYF2_AE

    Plasmid

    #148550

    Purpose

    Mammalian Expression of HsGIGYF2

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF2 (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-PE NG

    Plasmid

    #174554

    Purpose

    All-in-one prime editor piggyBac transposon, NG variant

    Depositor

    Tobias Cantz

    Article

    Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

    Insert

    SpCas9 NG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
    

    Use

    CRISPR and Synthetic Biology; Piggybac transposon

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    L1111R, G1218R, E1219F, A1322R, R1335V, T1337R

    Promoter

    CAG

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-EGFP-C1-HsGIGYF2_AE

    Plasmid

    #148577

    Purpose

    Mammalian Expression of HsGIGYF2

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    HsGIGYF2 (GIGYF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one silent mutation and one deletion of Q1211 com…

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPICZ:mTREK-1_cryst

    Plasmid

    #133269

    Purpose

    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    

    Use

    Tags

    SNS- 3C_cleavage_site-TAAA-GFP

    Expression

    Yeast

    Mutation

    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

    Promoter

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. FB013

    Plasmid

    #119710

    Purpose

    TU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Negative selection marker HSVtk (Pgpda:HSVtk:Ttub)
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTT5-hSDC1-CS2

    Plasmid

    #52367

    Purpose

    expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture.

    Depositor

    Gordon Laurie

    Article

    Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

    Insert

    SDC1 (SDC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R95Q and S194A (according to numbering in Fig. 2A…

    Promoter

    Available since

    Dec. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET28A-p53 (1-73) (P12A P13A)

    Plasmid

    #62066

    Purpose

    Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains TAD residues 1-73; P12A, P13A

    Promoter

    T7

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only