We narrowed to 9,370 results for: bli

1. NLuc CMV-Neo Flexi Vector pFC32K SARS-CoV2 5'Nsp1

   Plasmid

   #163319

   Purpose

   Encodes for first COVID protein - NSP1 fused to nano-luciferase

   Depositor

   Dawn Chandler

   Insert

   Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Luciferase

   Tags

   NanoLuc

   Expression

   Mammalian

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Myc-gamma3 (in pCDNA3.1)

   Plasmid

   #118041

   Purpose

   GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG3 (Gabrg3 Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Myc-gamma1 (in pCDNA3.1)

   Plasmid

   #118038

   Purpose

   GABAA receptor expression (gamma1 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG1 (Gabrg1 Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myc-gamma3 (in pCAGGS)

   Plasmid

   #118042

   Purpose

   GABAA receptor expression (gamma3 subunit) Myc-tag near N-terminus

   Depositor

   Angel de Blas

   Insert

   GABRG3 (Gabrg3 Rat)
   

   Tags

   cMyc epitope EQKLISEEDL inserted between fourth a…

   Expression

   Mammalian

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQStrep2-PIR

   Plasmid

   #31570

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   pirin (PIR Human)
   

   Tags

   His and StrepII-tag

   Expression

   Bacterial

   Available Since

   April 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF1-puro/hpb1(short)

   Plasmid

   #59973

   Purpose

   integrin b1 short headpiece expression (beta-I, hybrid, PSI domains) expression

   Depositor

   Timothy Springer

   Insert

   human integrin b1 (short) (ITGB1 Human)
   

   Tags

   Base coiled-coil and His-tag

   Expression

   Mammalian

   Promoter

   EF1a promoter

   Available Since

   Sept. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. a4 ecto pcDNA3.1 hygro(-)

   Plasmid

   #113406

   Purpose

   expression of human alpha 4 ectodomain with purification tags and C-terminal clasp

   Depositor

   Timothy Springer

   Article

   Li et al J Cell Biol. 2018 Jan 2;217(1):397-412.

   Insert

   human alpha 4 ectodomain
   

   Tags

   Strep II tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hs.KRAS4b G12A

   Plasmid

   #83142

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12A mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12A

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ANKRA2

   Plasmid

   #36904

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ANKRA2 (ANKRA2 Human)
   

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   amino acid residues 148-313 (ANK repeats)

   Available Since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

10. MS2_SC2_S_Lambda

    Plasmid

    #175048

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Lambda SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Lambda (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pMT-DREDD-V5-His

    Plasmid

    #63749

    Purpose

    Expresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    Death related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
    

    Tags

    V5-6XHis

    Expression

    Insect

    Promoter

    pMT

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIPZ (M)-HT-Cbx7-ATLm

    Plasmid

    #82522

    Purpose

    Lentivirus vector. Expresses mutant Halotag-Cbx7-ATLm fusion proteins in mammalian cells. Cbx7 ATL mutation of RKR70-72, K75, and KR77-78 to AGA70-72, A75, and AG77-78.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 7 (CBX7 Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    mutation of RKR70-72, K75, and KR77-78 to AGA70-7…

    Promoter

    CMV

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJK371

    Plasmid

    #73596

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope

    Depositor

    T. J. Kappock

    Inserts

    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    

    Tags

    FLAG tag and c-myc tag

    Expression

    Yeast

    Mutation

    Point mutants in FAD2-3 ORF relative to deposited…

    Promoter

    GAL1 and GAL10

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. HOXC6 (human) HIS-tag pET

    Plasmid

    #8565

    Depositor

    Corey Largman

    Insert

    HOXC6 (HOXC6 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    This protein starts at an internal Met initially …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pUASp YFP Rho1 DN

    Plasmid

    #52231

    Purpose

    Drosophila expression of YFP-Rho1 dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Rho1 (Rho1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T19N; DN mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pGEX2TK-YAP-WW1

    Plasmid

    #104305

    Purpose

    bacterial expression of WW domain 1 of YAP; binds PPxY where Y is not phosphorylated

    Depositor

    Marius Sudol

    Insert

    YAP WW1 domain (YAP1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    WW domain 1

    Promoter

    tac

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. MS2_SC2_S_Alpha

    Plasmid

    #175045

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Alpha SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Alpha (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. MS2_SC2_S_Beta

    Plasmid

    #175046

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Beta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Beta (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

    Plasmid

    #159913

    Purpose

    Mutagenesis of Kcnj6

    Depositor

    Larry Zweifel

    Insert

    Kcnj6 (Kcnj6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 4IA9

    Plasmid

    #51320

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5 (WDR5 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

21. HECW2

    Plasmid

    #32875

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HECW2 (HECW2 Human)
    

    Tags

    His tag (with TEV cleavage)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

22. pBACgus_Sumostar/LRRK2 1867-2161_C2024S/C2025S

    Plasmid

    #40394

    Depositor

    Michael J Fox Foundation MJFF

    Inserts

    SUMO
    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    6xHis and HisTEV

    Mutation

    aa1867-2161,C2024S/C2025S

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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23. 4PZI

    Plasmid

    #61955

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MLL4:JMC054-D08:C226821 (KMT2B Human)
    

    Tags

    6xHis and MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

24. Hs.KRAS4b C185S

    Plasmid

    #83139

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), C185S mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    C185S

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PIWIL2

    Plasmid

    #32042

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PIWIL2 (PIWIL2 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    PAZ domain only (amino acids 386-533)

    Available Since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

26. EPB41

    Plasmid

    #32040

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EPB41 (EPB41 Human)
    

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    FERM domain only (amino acids 1-279)

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

27. HSD17B8

    Plasmid

    #38853

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD17B8 (Hsd17b8 Human)
    

    Tags

    Tev His Flag

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

28. GHRHR

    Plasmid

    #39173

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    GHRHR (GHRHR Human)
    

    Use

    Baculovirus expression

    Tags

    Signal-His6-TEV

    Mutation

    N20->Q mutation of putative glycosylsation sit…

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pTEI134

    Plasmid

    #126071

    Purpose

    Vector for expression of plastid targeted YFP1-10 (T203Y) in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    YFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Gli3ZF in AINGpCAGGS

    Plasmid

    #121972

    Purpose

    Gli3 zinc fingers

    Depositor

    James Briscoe

    Insert

    Gli3ZF (GLI3 Human)
    

    Use

    Chick electroporation

    Tags

    5xmyc

    Expression

    Mammalian

    Mutation

    N terminus and C terminus deletion only nt 1411-1…

    Available Since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Hs.KRAS4b Q61K

    Plasmid

    #83164

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61K

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAdTrack-mLIP

    Plasmid

    #49197

    Purpose

    adenoviral shuttle vector with mouse C/EBPbeta LIP fragment

    Depositor

    Jed Friedman

    Insert

    C/EBPbeta (LIP fragment) (Cebpb Mouse)
    

    Use

    Adenoviral

    Mutation

    only the LIP fragment

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

33. Myc-gamma2s-EGFP

    Plasmid

    #118040

    Purpose

    GABAA receptor expression (gamma2 short subunit) double tagged, near N terminus and at C-terminus

    Depositor

    Angel de Blas

    Insert

    GABRG2 (Gabrg2 Rat)
    

    Tags

    EGFP and cMyc epitope EQKLISEEDL inserted between…

    Expression

    Mammalian

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA5/FRT/TO/Flag-tGFP-Cryz

    Plasmid

    #110378

    Purpose

    Mammalian expression of turboGFP-CRYZ with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    crystallin zeta (CRYZ Human)
    

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDNR-hSox2

    Plasmid

    #49389

    Purpose

    donor vector for cloning human Sox2 with 6xHN tag

    Depositor

    Randall McKinnon

    Insert

    Sox2 (SOX2 Human)
    

    Use

    Cre/Lox; Donor vector

    Tags

    6xHN (6 repeating His-Asn subunits)

    Mutation

    TGA (stop) to CCA (Pro) at nt 1027 with respect t…

    Promoter

    none

    Available Since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

36. Mouse Alpha2 delta-2 mutMIDAS HA pMT2

    Plasmid

    #58733

    Purpose

    expression of HA tagged mouse Alpha2 delta-2 with MIDAS site mutations in mammalian cells

    Depositor

    Annette Dolphin

    Insert

    alpha2 delta-2 (Cacna2d2 Mouse)
    

    Expression

    Mammalian

    Mutation

    MIDAS site mutations (D300VSGS to AVAGA)

    Promoter

    SV40

    Available Since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJK571

    Plasmid

    #75379

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits and associated hypothetical protein with N-terminal His6 tag (AaPurS, H6AaOrfY, AaPurQ, AaPurL-G209D)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Gene synthesis

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glycine-209 to aspartate and synthetic ge…

    Promoter

    n/a

    Available Since

    Aug. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBACgus N-His/GST/LRRK2 1867-2176

    Plasmid

    #40406

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    GST-Tev and HisTev

    Mutation

    WT aa1867-2176

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

    Plasmid

    #50888

    Purpose

    Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. GRM3 (3SM9)

    Plasmid

    #32870

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRM3 (GRM3 Human)
    

    Tags

    His tag with TEV cleavage and Honeybee melittin S…

    Expression

    Bacterial

    Available Since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits