We narrowed to 62,913 results for: cloning

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pTol1-UAS:CoChR-tdTomato

Plasmid

#124233

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CoChR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Publication

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

CoChR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSCM001

Plasmid

#124605

Purpose

Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH

Depositor

David Summers

Publication

Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1

Insert

mCherry-pHluorin

Tags

mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…

Expression

Bacterial

Promoter

pDAB

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

Plasmid

#66707

Purpose

expresses a single gRNA and VP64-dCas9-VP64

Depositor

Charles Gersbach

Inserts

hU6-gRNA expression cassette
S.Pyogenes VP64-dCas9-VP64

Use

Lentiviral and Synthetic Biology

Tags

VP64

Mutation

D10A and H840A

Promoter

hU6 and hUbC

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pAAV-DIO-dAPEX2

Plasmid

#117174

Purpose

Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Publication

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

dAPEX2

Use

AAV and Cre/Lox

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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HF-PX459 (V2)

Plasmid

#118632

Purpose

Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance

Depositor

Mike McGrew

Publication

Idoko-Akoh et al Sci Rep. 2018 Oct 11;8(1):15126. doi: 10

Insert

hSpCas9-HF1-2A-Puro V2.0

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Asparagine 497 to Alanine, Arginine 661 to Alanin…

Promoter

Cbh

Available since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLIX403_UBAP2L_mCherry

Plasmid

#105286

Purpose

puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein

Depositor

Eugene Yeo

Publication

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

UBAP2L (UBAP2L Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available since

Sept. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

Plasmid

#110057

Purpose

Per2 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Publication

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and Venus (Per2 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Per2

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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xCas9(3.6)-ABE(7.10)

Plasmid

#108387

Purpose

Mammalian expression vector for xCas9(3.6)-ABE

Depositor

David Liu

Publication

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-ABE(7.10)

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_T7-ARG2

Plasmid

#106474

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

Depositor

Mikhail Shapiro

Publication

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG2

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKK-TEV-CyOFP1

Plasmid

#105799

Purpose

Expression of your protein of interest in fusion with orange fluorescent protein at the C-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

Depositor

Andrzej Dziembowski

Publication

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-CyOFP1

Expression

Mammalian

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST14-Cys-SpyCatcher002

Plasmid

#102829

Purpose

Bacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagents

Depositor

Mark Howarth

Publication

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

Cys-SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Cysteine residue in N-terminal region

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEB1-mVenus NB

Plasmid

#103986

Purpose

Plasmid encoding mVenus NB

Depositor

Philippe Cluzel

Publication

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus NB

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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LAMP-mCherry-CRY2

Plasmid

#102249

Purpose

Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

Depositor

Bianxiao Cui

Publication

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

LAMP-mCherry-CRY2 (LAMP1, CRY2 Human, Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Publication

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLenti Lifeact-mRuby2 BlastR

Plasmid

#84384

Purpose

Lentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neurons

Depositor

Ghassan Mouneimne

Publication

Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

Insert

Lifeact

Use

Lentiviral

Tags

mRuby2

Promoter

CMV immediate early

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAct:dCas9-VPR

Plasmid

#78898

Purpose

Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.

Depositor

Norbert Perrimon

Publication

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VPR

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCas9-polyA

Plasmid

#72602

Purpose

express hCas9 byT7 promoter with polyadenine tails

Depositor

Tomoji Mashimo

Publication

Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

Insert

Cas9-polyadenine

Tags

81-bp polyadenylation

Promoter

T7

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pG1AK-sfGFP

Plasmid

#71739

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Publication

Reeve et al ACS Synth Biol. 2016 Jul 1.

Insert

sfGFP

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Promoter

pRplsWT

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-PGK-destination

Plasmid

#60436

Purpose

Destination vector with PGK - Hygromycin selection marker

Depositor

Austin Smith

Publication

Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

Type

Empty backbone

Expression

Mammalian

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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