We narrowed to 15,549 results for: pET

Showing: 8801 - 8850 of 15549 results

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plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR

Plasmid

#203963

Purpose

Dox inducible myc tag truncated SOX9 without trans-activating domain (noTA)

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9-noTA (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Mutation

the TA domain is truncated

Promoter

TRE3G

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR

Plasmid

#203964

Purpose

Dox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)

Depositor

Elaine Fuchs

Article

Yang et al Nat Cell Biol. 2023 Jul 24. doi: 10.1038

Insert

SOX9-noHMG (Sox9 Mouse)

Use

Lentiviral

Tags

myc

Mutation

the HMG domain is truncated

Promoter

TRE3G

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SBC015898

Plasmid

#226285

Purpose

Expresses PhCFAT, ObEGS1, and ObEOMT1 from trc promoter. Biosynthesis of methylated phenylpropenes from monolignol precursors.

Depositor

Rainer Breitling

Article

Chua et al Metab Eng Commun. 2024 May 15;18:e00237.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1
Eugenol O-methyltransferase

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAM221

Plasmid

#226645

Purpose

pET24b-p97-R191Q-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

R191Q

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM224

Plasmid

#226647

Purpose

pET24b-p97-T262A-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

T262A

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAM220

Plasmid

#226644

Purpose

pET24b-p97-R159H-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

R159H

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAM219

Plasmid

#226643

Purpose

pET24b-p97-R155H-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

R155H

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAM222

Plasmid

#226646

Purpose

pET24b-p97-L198W-His6

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Tags

His

Expression

Bacterial

Mutation

L198W

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAMC042z21

Plasmid

#209747

Purpose

RepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL bla

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

sfGFP - tRNA operon

Expression

Bacterial

Mutation

sfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pssAAV_Seq17_CMV_bglobin_3NLS_TFP_WPRE_polyA

Plasmid

#220223

Purpose

AAV-STARR-seq validation control vector with TFP

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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SCML2_3

Plasmid

#220878

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Article

Nady et al J Mol Biol. 2012 Nov 9;423(5):702-18. do

Insert

SCML2 (SCML2 Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac1-His6-E3(1)-CUbo-TwinStrep

Plasmid

#212712

Purpose

Expression of His6-E3(1)-CUbo-TwinStrep in insect cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

6xHis and CUbo-TwinStrep

Expression

Bacterial

Available Since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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NFAST(low)-Halo-ER

Plasmid

#214419

Purpose

ER localization of low-affinity NFAST fused to Halo-ER

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspA-N

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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GAL4(1-147)

Plasmid

#195029

Purpose

The control vector GAL4 (1-147) was generated by introducing a stop codon immediately after the GAL4 (1-147) sequence in the GAL4-Creb5 (1-128) vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

GAL4 (1-147)

Expression

Mammalian

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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GL4.10 Prg4 promoter-luciferase

Plasmid

#195034

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter (PRG4 Bovine)

Use

Luciferase

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R/K63R)-His6

Plasmid

#212780

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHyg-CUbo(K48R/K63R)-yeGFP

Plasmid

#212769

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-TET-NUbo-E3(63)-VSV

Plasmid

#212752

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-TET-NUbo-E3(63)-VSV

Plasmid

#212753

Purpose

Doxycycline-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-TET-NUbo-E3(48)-VSV

Plasmid

#212744

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-TET-NUbo-E3(48)-VSV

Plasmid

#212743

Purpose

Doxycycline-inducible expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

Tet

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-NUbo-E3(63)-FLAG

Plasmid

#212808

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(63)-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Tags

FLAG and NUbo

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-FLAG

Plasmid

#212797

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCaUra3-CUbo(K48R/K63R)-yeGFP

Plasmid

#212774

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R)-yeGFP-His6

Plasmid

#212783

Purpose

C-terminal PCR-based tagging with CUbo(K48R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K63R)-yeGFP-His6

Plasmid

#212784

Purpose

C-terminal PCR-based tagging with CUbo(K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R/K63R)-yeGFP-His6

Plasmid

#212785

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHyg-CUbo(K48R/K63R)-9myc

Plasmid

#212789

Purpose

C-terminal PCR-based tagging with CUbo(K48R/K63R)-9myc in budding yeast with HygR selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUra3-pCUP1-CUbo(K48R/K63R)

Plasmid

#212765

Purpose

N-terminal PCR-based tagging with pCUP1-CUbo(K48R/K63R) in budding yeast with URA3 selection

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Type

Empty backbone

Use

Pcr-based tagging

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-CUP1-NUbo-E3(63)-VSV

Plasmid

#212754

Purpose

Copper-inducible expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-ADH-NUbo-E3(63)-VSV

Plasmid

#212751

Purpose

Constitutive expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-ADH-NUbo-E3(63)-VSV

Plasmid

#212750

Purpose

Constitutive expression of NUbo-E3(63)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-ADH-NUbo-E3(48)-VSV

Plasmid

#212742

Purpose

Consitutive expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-ADH-NUbo-E3(48)-VSV

Plasmid

#212741

Purpose

Consitutive expression of NUbo-E3(48)-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-ADH-CUbo-VSV

Plasmid

#212733

Purpose

Consitutive expression of CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-ADH-CUbo-VSV

Plasmid

#212732

Purpose

Consitutive expression of CUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

CUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-CUP1-NUbo-VSV

Plasmid

#212730

Purpose

Copper-inducible expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

CUP1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp211-ADH-NUbo-VSV

Plasmid

#212729

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
YIp128-ADH-NUbo-VSV

Plasmid

#212728

Purpose

Consitutive expression of NUbo-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo

Use

Integrative vector

Tags

VSV

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-NUbo-E3(48)-FLAG

Plasmid

#212802

Purpose

Constitutive/Doxycycline-inducible expression of NUbo-E3(48)-FLAG in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48)

Tags

FLAG and NUbo

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST-3xFLAG-Ubc7

Plasmid

#212801

Purpose

Constitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc7

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Myc-E3(1)-CUbo

Plasmid

#212799

Purpose

Constitutive/Doxycycline-inducible expression of Myc-E3(1)-CUbo in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Tags

CUbo and Myc

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Ubc13

Plasmid

#212806

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc13

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-FRT-TO-Mms2

Plasmid

#212807

Purpose

Constitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Mms2

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-TEV2-Ubc7-His6

Plasmid

#212713

Purpose

Bacterial expression of MBP-Ubc7-His6, MBP-tag cleavable with TEV-protease

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

Ubc7

Tags

6xHis and MBP-TEV2

Expression

Bacterial

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TBPL1 KO 1

Plasmid

#200210

Purpose

gRNA for TBPL1 KO

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TBP1 KO 1 (Tbpl1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TBPL1 KO 2

Plasmid

#200211

Purpose

gRNA for TBPL1 KO

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TBP1 KO 2 (Tbpl1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA.3-V5-SPOP (R221C)

Plasmid

#197022

Purpose

Mammalian expression vecor for V5-tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

R221C

Available Since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA.3-V5-SPOP (R354H)

Plasmid

#197023

Purpose

Mammalian expression vecor for V5 tagged SPOP

Depositor

Tanja Mittag

Article

Sabri et al J Biol Chem. 2023 Dec;299(12):105427. do

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

R354H

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmGIGYF_641-1574_AH

Plasmid

#148936

Purpose

Insect Expression of DmGIGYF1_641-1574

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF1_641-1574 (Gyf Fly)

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 15,549 results for: pET