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pRK5_mCherry_P2AT2A_GFP-nb-KS

Plasmid

#238233

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hDlx_DIO_ChR2_mCherry

Plasmid

#224459

Purpose

An AAV vector expressing ChR2-mCherry that is dependent on both Cre and the hDlx enhancer

Depositor

Gordon Fishell

Article

Wu et al Nature. 2026 Jan 21. doi: 10.1038/s41586

Insert

ChR2-mCherry

Use

AAV

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

Plasmid

#191141

Purpose

Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:s70587-W:EGFP

Expression

Mammalian

Mutation

UAG>UGG (stop>Trp)

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNAHygro(+)LAMP2B

Plasmid

#86029

Purpose

Expresses LAMP2B in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Lysosome-associated membrane protein 2b (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS

Plasmid

#238243

Purpose

For integration of mCherry_P2AT2A_GFP-nb-2xKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1656 STITCHR Gluc AAVS1 payload

Plasmid

#234827

Purpose

Gluc exon 2 with 50bp AAVS1 homology arms

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

Gluc exon 2

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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CD8a

Plasmid

#86050

Purpose

A mammalian expression plasmid encoding CD8a without tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Expression

Mammalian

Promoter

CMV, T7

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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RBD#4L

Plasmid

#193021

Purpose

hnRNP C RRM RNA binding domain inserted in between N415 and N416 of Loop 1 of LwaCas13a

Depositor

Xue Gao

Article

Yang et al Nat Chem Biol. 2022 Sep 22. pii: 10.1038

Insert

Cas13a

Tags

hnRNP C RRM

Expression

Bacterial

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb-KS

Plasmid

#238240

Purpose

For integration of mCherry_P2AT2A_GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb

Plasmid

#238239

Purpose

For integration of mCherry_P2AT2A_GFP-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-YY1

Plasmid

#104395

Purpose

HA-YY1

Depositor

Richard Young

Article

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Insert

YY1 (YY1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Squash-Broccoli

Plasmid

#177918

Purpose

Expresses a bifunctional circular RNA with Squash in one arm and Broccoli in the other arm of the F30 scaffold, in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Squash-Broccoli

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMA-Thermal_CTLA-4

Plasmid

#199751

Purpose

Thermal expression of CTLA-4

Depositor

Mikhail Shapiro

Article

Abedi et al Nat Commun. 2022 Mar 24;13(1):1585. doi:

Insert

CTLA-4 (Ctla4 Mouse)

Expression

Bacterial

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Plasmid

#238244

Purpose

For integration of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS_FtoG

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Plasmid

#177920

Purpose

Expresses ratiometric Squash-SAM sensor 5-1 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 5-1

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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CD8a-EGFP

Plasmid

#86051

Purpose

A mammalian expression plasmid encoding CD8a with a C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

CD8a (CD8A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry_P2AT2A_GFP-nb-KS-FtoG

Plasmid

#238235

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDY1513 AAVS1 sgRNA

Plasmid

#234832

Purpose

sgRNA for AAVS1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

AAVS1 sgRNA

Use

CRISPR

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin WT

Plasmid

#50856

Purpose

expresses human cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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