We narrowed to 5,445 results for: SPI

1. pET-His-SCov2-nsp7

   Plasmid

   #154757

   Purpose

   Expression of SARS-CoV-2 nsp7

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-puro-eGFP-HEC1

   Plasmid

   #114022

   Purpose

   Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1 - Wildtype (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/HisC-mTAZ

   Plasmid

   #31793

   Depositor

   Michael Yaffe

   Article

   Hong et al Science. 2005 Aug 12;309(5737):1074-8.

   Insert

   TAZ (Wwtr1 Mouse)
   

   Use

   Tags

   EK (Enterokinase) Recognition Site, His, and Xpre…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-His-SCov2-nsp8

   Plasmid

   #154758

   Purpose

   Expression of SARS-CoV-2 nsp8

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. LbCas12a-D156R

   Plasmid

   #213744

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-D156R in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1 beta-catenin Y654F

   Plasmid

   #16072

   Depositor

   Erin Schuman

   Article

   Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

   Insert

   beta-catenin (CTNNB1 Chicken)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Y654F, *(see below)

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046899

   Plasmid

   #145608

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAC1430

   Plasmid

   #127981

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FCK-Halo-GFP

   Plasmid

   #14750

   Purpose

   Lentiviral expression of Halo-GFP for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al PLoS One. 2007 Mar 21;2(3):e299.

   Insert

   Mammalian codon-optimized halorhodopsin, from Natronomas pharaonis (N. pharaonis), fused with GFP at the C-terminus
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N. pharaonis halorhodopsin is mammalian codon-opt…

   Promoter

   CaMKIIa

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. H6hSDHA

    Plasmid

    #113045

    Purpose

    Hetero expression of His-tagged human SDHA

    Depositor

    Edward A. Berry

    Article

    Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

    Insert

    H6hSDHA (SDHA Human)
    

    Use

    Tags

    His6 (MGSSHHHHHHSQDP-)

    Expression

    Bacterial

    Mutation

    Insert begins at amino acid 43

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-IRES-GFP-mouse-Caspase-11

    Plasmid

    #183358

    Purpose

    Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-11 (Casp4 Mouse)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HA-14-3-3 ϵ

    Plasmid

    #48797

    Purpose

    expresses human myc-14-3-3ϵ in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Insert

    YWHAE 14-3-3 ϵ (YWHAE Human)
    

    Use

    Tags

    haemagglutinin

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

13. LbdCas12a KRAB

    Plasmid

    #188503

    Purpose

    Expresses FLAG tagged LbdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-Brn2

    Plasmid

    #32925

    Purpose

    Retroviral expression of mouse Brn2

    Depositor

    Kevin Eggan

    Article

    Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

    Insert

    Brn2 (Pou3f2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

15. CAG_mCh_p85a_mCh

    Plasmid

    #199630

    Purpose

    FRET acceptor for IGF1R sensor

    Depositor

    Ryohei Yasuda

    Article

    Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

    Insert

    p85a-SH2 (Pik3r1 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. His6-PRC1

    Plasmid

    #69111

    Purpose

    For bacterial expression of recombinant human PRC1 tagged with His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Protein Regulator of Cytokinesis 1 (PRC1 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MTK Lentivirus Single Cassette Destination Vector (pAN2414)

    Plasmid

    #194220

    Purpose

    Backbone for constructing single casette expression plasmids using the Mammalian Tookit

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV4a-SIRT6-FLAG

    Plasmid

    #102322

    Purpose

    Human SIRT6 with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p-mCherry2-sgRNA (empty)

    Plasmid

    #198330

    Purpose

    Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    U6-sgRNA(F+E) empty
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVenus-C1-Cdc20 (1534)

    Plasmid

    #26058

    Depositor

    Jonathon Pines

    Article

    Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

    Insert

    Cdc20 (Cdc20 Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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