159,557 results

1. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   EPFL Protein Production and Structure Core Facility (PTPSP), Florence Pojer

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-DJ-N589X

   Plasmid

   #130878

   Purpose

   Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

   Depositor

   Prashant Mali

   Article

   Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

   Insert

   AAV-DJ cap N589X
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N589X

   Promoter

   Unknown

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CMV-Blast-PIP-FUCCI

   Plasmid

   #138715

   Purpose

   Lentiviral expression of the cell cycle marker PIP-FUCCI

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-FUCCI
   

   Use

   Lentiviral

   Tags

   mCherry and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Flpe

   Plasmid

   #13787

   Purpose

   Mammalian expression of enhanced form of Flp recombinase

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Flpe
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. FUGW-PercevalHR

   Plasmid

   #49083

   Purpose

   Lentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330 p53

   Plasmid

   #59910

   Purpose

   pX330 backbone expressing sgRNA targeting p53 to edit mouse p53. Expresses Cas9 from CBh promoter

   Depositor

   Tyler Jacks

   Article

   Xue et al Nature. 2014 Aug 6. doi: 10.1038/nature1

   Insert

   sgRNA targeting p53 (Trp53 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe puro HA PIK3CA H1047R

   Plasmid

   #12524

   Purpose

   Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

   Depositor

   Jean Zhao

   Article

   Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

   Insert

   PIK3CA H1047R (PIK3CA Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H1047R. Tumor-derived allele.

   Promoter

   Available since

   Aug. 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmiRFP670-N1

   Plasmid

   #79987

   Purpose

   Monomeric near-infrared fluorescent protein miRFP670 in mammalian plasmid

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

   Has Service

   Cloning Grade DNA

   Insert

   miRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3120 pSFFV-neo Bcl-XL

   Plasmid

   #8749

   Depositor

   Stanley Korsmeyer

   Article

   Chao et al J Exp Med 1995 Sep 1;182(3):821-8.

   Insert

   Bcl-xl (BCL2L1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. LVXN-Neo-NSD2

    Plasmid

    #86010

    Purpose

    express wild-type NSD2 in mammalian cells

    Depositor

    Darrin Stuart

    Article

    Jaffe et al Nat Genet. 2013 Nov;45(11):1386-91. doi:

    Insert

    NSD2 (NSD2 Human)
    

    Use

    Lentiviral

    Tags

    Flag tag D Y K D D D D K

    Expression

    Bacterial

    Mutation

    K1002R (please see depositor comments below)

    Promoter

    CMV

    Available since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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