We narrowed to 21,813 results for: Sis

1. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

   Plasmid

   #127365

   Purpose

   Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Tags

   GS linker, NLS, V5, and YFP

   Expression

   Plant

   Mutation

   R118G

   Promoter

   Arabidopsis UBQ10 promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZJQIBEBT003

   Plasmid

   #140053

   Purpose

   The pZJQIBEBT003 plasmid contains the coding sequence of eMutS-L157C-G233C gene which has the ability of error removal in the gene synthesis process.

   Depositor

   Jia Zhang

   Article

   Zhang et al ACS Synth Biol. 2020 Apr 17;9(4):940-952

   Insert

   eMutS-L157C-G233C-CBM-EGFP
   

   Use

   Tags

   His-Tag

   Expression

   Bacterial

   Mutation

   L157C, G233C

   Promoter

   T7

   Available since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. sox10:PDGFRA wild-type

   Plasmid

   #98696

   Purpose

   I-SceI meganuclease-mediated human PDGFRA wild-type transgenesis in zebrafish

   Depositor

   Thomas Look

   Article

   Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

   Insert

   PDGFRA (PDGFRA Human)
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2Apuro-TRE-synPCB2.0

   Plasmid

   #139481

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
   

   Use

   Tol2 transposon donor vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Tet responsive element

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFRT-TODestFLAGHA_RBPMS_KDR_HindIII

   Plasmid

   #65662

   Purpose

   FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

   Depositor

   Thomas Tuschl

   Article

   Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

   Insert

   RBPMS (RBPMS Human)
   

   Use

   Tags

   FLAG HA

   Expression

   Mammalian

   Mutation

   introduced HindIII site (final goal to exchange F…

   Promoter

   CMV, 2x Tet

   Available since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. p35S::IRE1a

   Plasmid

   #135232

   Purpose

   constitutive expression of IRE1a from A. thaliana (AT2G17520; upregulates UPR signaling)

   Depositor

   Armin Djamei

   Article

   Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

   Insert

   IRE1a (IRE1A Mustard Weed)
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. R4pGWB601_UBQ10p-BirA(R118G)-NES-YFP

   Plasmid

   #127363

   Purpose

   Binary vector for expressing cytosolic BirA* (R118G)-YFP under the UBQ10 promoter in plants

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   BirA* (R118G mutant)
   

   Use

   Tags

   GS linker, NES, V5, and YFP

   Expression

   Plant

   Mutation

   R118G

   Promoter

   Arabidopsis UBQ10 promoter

   Available since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCA-G-intron(Neo)-T(FLLFLNL)

   Plasmid

   #40020

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tdT-3Myc
   beta-globin intron
   Neo
   

   Use

   Tags

   3 Myc tags

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta globin promoter and CMV enhance…

   Available since

   Oct. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1-SLC35B2-FLAG

   Plasmid

   #154863

   Purpose

   Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 (SLC35B2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   ctcctggtgcagtacttc => ttattagtccaatatttt

   Promoter

   CMV

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sox10:PDGFRA mutant

    Plasmid

    #98697

    Purpose

    I-SceI meganuclease-mediated human PDGFRA mutant transgenesis in zebrafish

    Depositor

    Thomas Look

    Article

    Ki et al Oncogene. 2017 Feb 23;36(8):1058-1068. d

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    exon 8 and 9 deletion

    Promoter

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJY-RpABE-FT_INF

    Plasmid

    #112874

    Purpose

    binary vector for INF (Induced Neo-Functionalization) of FT in Arabidopsis

    Depositor

    Jin-Soo Kim, Jae-Young Yun

    Article

    Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

    Inserts

    RPS5A promoter
    ABE7.10
    U6-sgRNA targeting FT
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7

    Plasmid

    #124859

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-Coh-ddFLN4-ELP-HIS-ybbr

    Plasmid

    #153446

    Purpose

    E. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc.CohE
    

    Use

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBT250_(pCA-G-intron(Neo)-tTA2)

    Plasmid

    #36876

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCA-G-intron(Neo)-T(LNL)

    Plasmid

    #36907

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Neo
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHyg-AID(1-114)

    Plasmid

    #99513

    Purpose

    Truncated C-terminal AID degron cassette with HygR marker for S. cerevisiae

    Depositor

    Helle Ulrich

    Article

    Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

    Insert

    IAA17(1-114) (AXR3 Mustard Weed)
    

    Use

    Pcr template for yeast tagging cassette

    Tags

    Expression

    Mutation

    internal inversion of nucleotides 265-312 of the …

    Promoter

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWZL-rCHES1

    Plasmid

    #51847

    Purpose

    encodes a CHES1 mRNA that is resistant to shRNA against CHES1

    Depositor

    Gerardo Ferbeyre

    Article

    Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

    Insert

    CHES1 (FOXN3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2014

    Availability

    Academic Institutions and Nonprofits only

18. UBQ10pro-nlsABACUS2-400n-NosT

    Plasmid

    #203728

    Purpose

    Binary vector for the transformation of the nlsABACUS2-400n biosensor for (+)-Abscisic acid into Arabidopsis

    Depositor

    Alexander Jones

    Article

    Rowe et al Nat Plants. 2023 Jun 26. doi: 10.1038/s4

    Insert

    nlsABACUS2-400n
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10pro 1500bp

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHEE901-A3A

    Plasmid

    #229044

    Purpose

    nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.

    Depositor

    Huawei Zhang

    Article

    Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. PBE-NG

    Plasmid

    #229045

    Purpose

    Modified nCas9 mediated-base editing with NG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R in Cas9.

    Depositor

    Huawei Zhang

    Article

    Wang et al Genome Biol. 2024 Aug 9;25(1):215. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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