We narrowed to 6,494 results for: aav

1. pAAV-Ef1a-FAS-ChETA-TdTomato-WPRE-pA

   Plasmid

   #37089

   Purpose

   Expresses ChETA-tdTomato in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic excitation

   Depositor

   Bernardo Sabatini

   Article

   Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

   Insert

   ChETA-TdTomato
   

   Use

   AAV and Cre/Lox; Cre-off

   Tags

   TdTomato

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA

   Plasmid

   #158989

   Purpose

   Vector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9-SID4X
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pMecp2

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-FLEX-tmeGFP-IRES-nls-mRuby2

   Plasmid

   #63060

   Purpose

   Expresses truncated monomeric eGFP and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   nls-mRuby2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1531_pAAV-U6-SA-Self1-gRNA-HLP-EmGFP-spA

   Plasmid

   #109318

   Purpose

   Plasmid for liver-specific expression of EmGFP with a gRNA against SaCas9

   Depositor

   William Lagor

   Article

   Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

   Insert

   SaCas9 RuvC gRNA
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE

   Plasmid

   #186047

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8f
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CAG-FLEX-LifeAct-jGCaMP8m-WPRE

   Plasmid

   #186048

   Purpose

   AAV-mediated expression of LifeAct-jGCaMP8m under the CAG promoter, Cre-dependent expression

   Depositor

   Loren Looger

   Insert

   LifeAct-jGCaMP8m
   

   Use

   AAV

   Tags

   LifeAct-6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-LMO4 (GLucM23-VChR1-EYFP)

   Plasmid

   #114101

   Purpose

   fusion protein of Gaussia luciferase variant M23, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Park et al J Neurosci Res. 2017 Sep 1. doi: 10.1002

   Insert

   Gaussia luciferase, M23; Volvox Channelrhodopsin 1; EYFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOTTC710 - pAAV EF1a DIO Mem-AcGFP

   Plasmid

   #75081

   Purpose

   An AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

   Insert

   Mem-AcGFP
   

   Use

   AAV and Cre/Lox

   Tags

   Palmitylation site

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAGGS-AsCpf1(D908A)-2A-GFP-U6-AAVS1-sgRNA

   Plasmid

   #194727

   Purpose

   CAGGS-AsCpf1(D908A)-2A-GFP-U6-sgRNA-cloning vector, with guide RNA array targeting hAAVS1.

   Depositor

   Rudolf Jaenisch

   Article

   Haiting et al bioRxiv

   Insert

   AsCpf1(D908A) (AAVS1 Acidaminococcus sp.)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CMV.SV40.THBS1-deltaTSR3-CTD-HA.SV40(polyA)

    Plasmid

    #155194

    Purpose

    Expresses truncated (AA 1-690) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Thrombospondin-1 (Thbs1 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Truncated sequence of THBS1 (expresses Amino Acid…

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-Linker-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120391

    Purpose

    pAAV plasmid expressing mCherry.3XFLAG under the hSyn promoter with a Linker containing BstBI, BspQI and SacI sites upstream of the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Type

    Empty backbone

    Use

    AAV

    Tags

    mCherry.3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1α1.1-Archon1-KGC-GFP-ER2

    Plasmid

    #115890

    Purpose

    AAV-mediated expression of Archon1-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version). Using SV40 signal.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    Archon1-KGC-GFP-ER2
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-FAS-NpHR3.0-eYFP-WPRE-pA

    Plasmid

    #37088

    Purpose

    Expresses NpHR3.0-EYFP in cells lacking Cre (Cre-Off, flanked by alternative lox FAS sites), for optogenetic inhibition

    Depositor

    Bernardo Sabatini

    Article

    Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

    Insert

    NpHR3.0-EYFP
    

    Use

    AAV and Cre/Lox; Cre-off

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-Coff/Fon-bREACHes-EYFP

    Plasmid

    #137147

    Purpose

    Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    Coff/Fon-bREACHes-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

    Plasmid

    #193915

    Purpose

    Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    miniSOG
    

    Use

    AAV

    Tags

    Farnesylation signal sequence and Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

    Plasmid

    #186417

    Purpose

    Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

    Depositor

    Mary Kay Lobo

    Article

    Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

    Insert

    Early growth response 3 (Egr3 Mouse)
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mutation

    Promoter

    EF-1-alpha

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE

    Plasmid

    #120393

    Purpose

    pAAV plasmid expressing Diras2 shRNA1 under the U6 promoter and mCherry.3XFLAG under the hSyn promoter

    Depositor

    Florian Freudenberg

    Article

    Grunewald et al J Atten Disord. 2019 Jan 9:1087054718822

    Insert

    Diras2 shRNA (Diras2 Mouse)
    

    Use

    AAV and RNAi

    Tags

    mCherry.3XFLAG

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-Ef1a-DIO C1V1 (t/t)-TS-mCherry (PV2670)

    Plasmid

    #100061

    Purpose

    AAV expression of ChR1-VChR1 Chimera fused to mCherry driven by Ef1a promoter

    Depositor

    Karl Deisseroth

    Has Service

    AAV9

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    EF1a

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-EF1a-NES-tdTomato-LINuS-WPRE

    Plasmid

    #159894

    Purpose

    AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

    Depositor

    Tatsushi Onaka

    Article

    Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

    Insert

    NES-tdTomato-LINuS
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α promoter

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV hSyn1 bPAC cMyc T2A tDimer

    Plasmid

    #85397

    Purpose

    humanized photoactivated adenylyl cyclase with cMyc Tag driven by neuron-specific promoter, plus red fluorescent protein

    Depositor

    Thomas Oertner

    Inserts

    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    

    Use

    AAV

    Tags

    cMyc Tag

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin-1 and ribosomal skip sequence T2A

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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