We narrowed to 165,343 results for: addgene

1. GFAP-Cre

   Plasmid

   #24704

   Depositor

   Michael Sofroniew

   Article

   Bush et al Neuron. 1999 Jun . 23(2):297-308.

   Insert

   Cre
   

   Expression

   Mammalian

   Promoter

   GFAP (glial fibrillary acidic protein)

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-EF1a-GFP ArgSen

   Plasmid

   #184704

   Purpose

   Lentiviral transfer plasmid for expression of GFP ArgSen

   Depositor

   Brian Van Tine

   Article

   Rogers et al Cancer Metab. 2021 Jan 21;9(1):4. doi: 1

   Insert

   GFP ArgSen
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLM3705

   Plasmid

   #47754

   Purpose

   Expresses mammalian cell codon-optimized dCas9-VP64

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   codon optimized dCas9-VP64
   

   Use

   CRISPR

   Tags

   3x FLAG, NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A and H840A mutations in Cas9 (catalytically i…

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFGC5941DR5-PacI-ath-STTM164

   Plasmid

   #84090

   Purpose

   target miRNA164 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM164
   

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROPseq-Puro-24xMS2

   Plasmid

   #212629

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFGC5941-PacI-ath-STTM319

   Plasmid

   #84115

   Purpose

   target miRNA319 for destruction in Arabidopsis thaliana

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Insert

   STTM319
   

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. UPB-pancreas (UPB7)

   Plasmid

   #180787

   Purpose

   Unique Projection Barcode (UPB7)-Pancreas for Projection-seq

   Depositor

   Rui Chang

   Article

   Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

   Insert

   hM4D(Gi) (partial )
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC3-EGFR

   Plasmid

   #222610

   Purpose

   Expression of MAC3-tagged EGFR

   Depositor

   Markku Varjosalo

   Article

   Liu et al Methods Mol Biol. 2023;2690:281-297. doi

   Insert

   EGFR (EGFR Human)
   

   Tags

   MAC3

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Aug. 5, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pJV53-Cas12a

   Plasmid

   #158706

   Purpose

   CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1

   Insert

   FnCpf1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   TetO

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEC726

    Plasmid

    #190814

    Purpose

    CRISPRi backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    S. pyogenes dCas9 codon optimized for Streptomyce…

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDestattB_ubi:EGFP (pCM272)

    Plasmid

    #68339

    Purpose

    Multisite Gateway destination vector for phiC31-mediated transgenesis in zebrafish, harbors ubiquitin promoter (ubi)-driven EGFP.

    Depositor

    Christian Mosimann, Leonard Zon

    Article

    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10

    Insert

    EGFP
    

    Use

    Zebrafish transgenesis

    Promoter

    ubi

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-Rab13 in pCSDEST2

    Plasmid

    #140879

    Purpose

    Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab13 (rab13 Zebrafish)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKD4-Nter-APEX2

    Plasmid

    #112868

    Purpose

    Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

    Depositor

    Eric Cascales

    Article

    Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

    Insert

    APEX2
    

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMMP-LMP1-IRES-eGFP

    Plasmid

    #36955

    Depositor

    Bill Sugden

    Article

    Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

    Insert

    LMP1 (LMP-1 )
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSicoR (Puro) p65 shRNA1

    Plasmid

    #22507

    Depositor

    Tyler Jacks

    Article

    Meylan et al Nature. 2009 Nov 5;462(7269):104-7. doi:

    Insert

    p65 shRNA1 (Rela Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hsyn-FLPo-T2A-GFP

    Plasmid

    #161766

    Purpose

    express FLPo and GFP under the control of human synapsin promoter

    Depositor

    Baoji Xu

    Article

    An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

    Inserts

    FLPo
    T2AEGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynapsin1

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. mc2 mNeon hPGK

    Plasmid

    #206230

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Brett Stringer, Simon Conn

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiGuide_mCherry_NT135

    Plasmid

    #183121

    Purpose

    pLentiGuide modified to express mCherry and a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. RIPK4_pCSdest

    Plasmid

    #53897

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    RIPK4 (RIPK4 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pBRα-σ70 D581G

    Plasmid

    #53732

    Purpose

    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    

    Expression

    Bacterial

    Mutation

    The sigma 70 moiety carries the D581G substitution

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only