We narrowed to 170,492 results for: addgene

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PX459-sgRNA048_Grb10

Plasmid

#232934

Purpose

Expression vector for a sgRNA against the mouse Grb10 locus and SpCas9 with T2A-Puromycin resistance gene.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

Cas9-T2A-PuroR (Grb10 S. pyogenes)

Expression

Mammalian

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMB3-B-TelR

Plasmid

#204992

Purpose

Level 3 MoClo plasmid for A. baumannii with additional Tellurite resistance cassette for multi-drug resistance strains

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

mRFP1

Use

Synthetic Biology

Expression

Bacterial

Mutation

Added replications elements for Acinetobacter spe…

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pBABE Hygro ST6GalNaC5

Plasmid

#26981

Depositor

Joan Massague

Article

Bos et al Nature. 2009 Jun 18. 459(7249):1005-9.

Insert

ST6GalNaC5 (ST6GALNAC5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
PRDM15_pCSdest

Plasmid

#53907

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PRDM15 (PRDM15 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
mc2 puro EF1a

Plasmid

#206221

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_TMv2_Zeo

Plasmid

#131762

Purpose

frameshift reporter for barcode retrieval (with Zeo selection)

Depositor

Paul Blainey

Article

Feldman et al BMC Biol. 2020 Nov 24;18(1):177. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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THI VP64 pALD4 T-

Plasmid

#161481

Purpose

Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Non targeting control NTC)

Expression

Yeast

Promoter

pALD4, pPOR1, and pTEF2

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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mEm-CoilE-Actin

Plasmid

#181993

Purpose

CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

mEm-CoilE-Actin

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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CEBPalpha NGFR

Plasmid

#44627

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

CEBPalpha (Cebpa Mouse)

Use

Retroviral

Tags

IRES and truncated NGFR

Expression

Mammalian

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pCOLA-Spec-yibDp-Erv1p

Plasmid

#212094

Purpose

Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

Sulfydryl oxidase (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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N-terminal Halo-XLF

Plasmid

#207546

Purpose

Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pWPXL-NP1

Plasmid

#72654

Purpose

Constitutive expression of NP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

NP1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-Src-Dasatinib-resistant

Plasmid

#26980

Depositor

Joan Massague

Article

Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

Insert

c-Src (SRC Chicken)

Use

Retroviral

Expression

Mammalian

Mutation

T338I

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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Gfi1 NGFR

Plasmid

#44630

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

Gfi1 (Gfi1 Mouse)

Use

Retroviral

Tags

IRES and truncated NGFR

Expression

Mammalian

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
sgRNA1_Tet-inducible Luciferase reporter

Plasmid

#64161

Purpose

Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

sgRNA1 for Tet-inducible Luciferase reporter

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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mc2 mCherry EF1a

Plasmid

#206225

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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TAL2431

Plasmid

#36830

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

HDAC2-TALEN_Right (Hdac2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
PFKL_pCSdest

Plasmid

#53901

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PFKL (PFKL Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
miR-vector-RFP pCDNA

Plasmid

#97143

Purpose

for expression of miRNA and TagRFP

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Tags

TagRFP

Expression

Mammalian

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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MRAP_pCSdest

Plasmid

#53833

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

MRAP (MRAP Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits