We narrowed to 5,294 results for: codon optimized

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pMXs_FLAG-Sfxn2 (CG6812)

Plasmid

#110643

Purpose

Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

CG6812 (CG6812 Fly)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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hSRAP.HMG6.wt.Dlnk.a13-236

Plasmid

#49814

Purpose

expression vector for MBP-SRAP(13-236) fusion

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein (SRA1 Human)

Tags

E. coli Maltose Binding Protein

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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HsCLYBL(aa23-340) D320N-His

Plasmid

#111289

Purpose

bacterial expression

Depositor

Vamsi Mootha

Article

Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

Insert

human CLYBL ( aa23-340) D320N codon optimized for bacterial expression (CLYBL Human)

Tags

His tag

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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lvl0 TagRFP CTAG

Plasmid

#153401

Purpose

Red fluorescent protein CTAG codon optimised for Arabidopsis thaliana

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

TagRFP CTAG

Expression

Plant

Available Since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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lvl0 YPet CTAG

Plasmid

#153402

Purpose

Yellow fluorescent protein CTAG codon optimised for Arabidopsis thaliana

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

Ypet CTAG

Expression

Plant

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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lvl0 eYFP CTAG

Plasmid

#153396

Purpose

Yellow fluorescent protein CTAG codon optimised for Arabidopsis thaliana

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

eYFP CTAG

Expression

Plant

Available Since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-SA-CAG-Pa01-BFP

Plasmid

#234142

Purpose

Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Insert

Pa01

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCas9-EGFP

Plasmid

#63592

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Phil Sharp, Feng Zhang

Article

Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

Inserts

Cas9
EGFP

Use

CRISPR and Lentiviral

Tags

FLAG and P2A

Expression

Mammalian

Promoter

EFS-NS

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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T7opt in pCAGGS

Plasmid

#65974

Purpose

Human codon-optimized T7 polymerase

Depositor

Benhur Lee

Article

Yun et al J Virol. 2015 Jan 15;89(2):1242-53. doi:

Insert

T7-opt

Expression

Mammalian

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-hMLH1dn for IVT

Plasmid

#178114

Purpose

Template for in vitro transcription of human MLH1dn

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1dn (codon optimized) (MLH1 Human)

Use

Template for in vitro transcription

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEf1a-Dn29-dCas9-P2A-GFP

Plasmid

#247155

Purpose

Mammalian expression of a human codon optimized wildtype Dn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

Dn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-superDn29-P2A-GFP

Plasmid

#247152

Purpose

Mammalian expression of a human codon optimized engineered superDn29 recombinase (highest efficiency variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

superDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/I233K/K248R/I303K/Q332K/N341…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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ATG-2460

Plasmid

#108713

Purpose

EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

Depositor

Keith Wood

Article

Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

Insert

CBR2opt-copGFP fusion

Use

Lentiviral

Tags

CBR2opt fused to copGFP

Mutation

Promega used directed evolution to create CBR (Cl…

Promoter

elongation factor-1

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEf1a-goldDn29-P2A-GFP

Plasmid

#247153

Purpose

Mammalian expression of a human codon optimized engineered goldDn29 recombinase (efficient and specific variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

goldDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pEf1a-hifiDn29-P2A-GFP

Plasmid

#247154

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase (highest specificity variant)

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-P2A-GFP

Tags

6xHis

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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eeBxb1

Plasmid

#222339

Purpose

Plasmid for expressing evolved and engineered Bxb1 in the mammalian cells

Depositor

David Liu

Article

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

Codon-optimized evolved and engineered Bxb1

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pKSE401

Plasmid

#62202

Purpose

CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Kan resistance

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Inserts

gRNA scaffold
zCas9

Use

Plant binary vector

Tags

3x FLAG and NLS

Expression

Plant

Mutation

maize codon optimized

Promoter

35S and AtU6-26p

Available Since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pIA123

Plasmid

#234038

Purpose

E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.

Depositor

Craig Ellermeier

Article

Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Inserts

Cas9
sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

Pgdh and Pxyl

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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CBS-7169

Plasmid

#253633

Purpose

MpCas12a2 expression

Depositor

Chase Beisel

Article

Dmytrenko et al Nature. 2026 Jan;649(8099):1312-1321. do

Insert

MpCas12a2 E. coli codon optimized C-terminal His tag

Expression

Bacterial

Mutation

N/A

Promoter

T7

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

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AAV pEF1a-DIO-FLPo-WPRE-hGHpA

Plasmid

#87306

Purpose

An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

Depositor

Li Zhang

Article

Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

Insert

Cre-dependent mouse codon-optimized Flp recombinase.

Use

AAV

Tags

N/A

Expression

Mammalian

Promoter

EF1a

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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