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pINDUCER20-FLAG-hMYOD1 (#800)

Plasmid

#78330

Purpose

Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a FLAG tag

Depositor

Matthew Alexander, Louis Kunkel

Insert

MYOD1 (MYOD1 Human)

Use

Lentiviral

Tags

FLAG

Promoter

TRE2

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCasY1

Plasmid

#87687

Purpose

p15A plasmid expressing CasY1

Depositor

Jennifer Doudna

Article

Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

Insert

CasY1

Expression

Bacterial

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304_CdTag-HA_EmptyVector

Plasmid

#221466

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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WNV VLPs pVRC8400

Plasmid

#63160

Purpose

West Nile Virus prM-E structural cassette (NY99 sequence) in pVRC8400

Depositor

Stephen Harrison

Article

Chao et al Elife. 2014 Dec 5;4. doi: 10.7554/eLife.

Insert

WNV prM-E

Expression

Mammalian

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEZYegfp

Plasmid

#18671

Purpose

pEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cells

Depositor

Yu-Zhu Zhang

Article

Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

Type

Empty backbone

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2008

Availability

Academic Institutions and Nonprofits only

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pLX311_CdTag-HA_EmptyVector

Plasmid

#221468

Purpose

Gateway backbone for C-terminal dTag cloning

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Type

Empty backbone

Use

Lentiviral

Tags

FKBP F36V

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAG425GAL-ccdB

Plasmid

#14153

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pBAD33.lon

Plasmid

#22145

Depositor

Robert Sauer

Article

Gur et al Genes Dev. 2008 Aug 15. 22(16):2267-77.

Insert

Lon (lon E. coli K12)

Expression

Bacterial

Mutation

None, WT gene

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pCFJ212 - pDESTR4-R3(cxTi10816, IV)

Plasmid

#34864

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

cxTi10816 targeting

Use

Worm targeting

Available Since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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p5-39

Plasmid

#118088

Purpose

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

FKBP-TSGGSG-Split C-terminal T7 RNAP expression plasmid

Use

Synthetic Biology

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pJMP2844

Plasmid

#160675

Purpose

Mobile CRISPRi empty sgRNA (BsaI) for cloning new sgRNAs

Depositor

Jason Peters

Article

Banta et al Curr Protoc Microbiol. 2020 Dec;59(1):e1

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGCS-C5(GST)

Plasmid

#85728

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

GST

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pIB165

Plasmid

#90188

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAG413GAL-ccdB

Plasmid

#14141

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway destination

Expression

Yeast

Available Since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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pSC101-ΔPS-PM,CS-RBSBBa_B0034-geneVI

Plasmid

#134356

Purpose

Accessory plasmid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Gene VI

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGART-mCherry

Plasmid

#167164

Purpose

Expresses human GART-mCherry fusion in mammalian cells

Depositor

Stephen Benkovic

Insert

GART (GART Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDZ50

Plasmid

#41948

Depositor

Stephen Elledge

Article

Zhang et al Cell. 2006 Aug 11;126(3):529-42.

Insert

ubiquitin specific peptidase 28 (USP28 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2013

Availability

Academic Institutions and Nonprofits only
373 pTol2-fli1ep:CherryDest

Plasmid

#73493

Purpose

Tol2 vector with fli1ep element upstream of mCherry, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:mCherry-attR1-attR2

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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PLlacO-1-CImf

Plasmid

#40126

Depositor

Michel Maharbiz

Article

Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

Insert

Lac inducible lambda repressor

Use

Synthetic Biology

Tags

mf-lon ssrA tag

Expression

Bacterial

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only

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372 pTol2-fli1ep:EGFPDest

Plasmid

#73491

Purpose

Tol2 vector with fli1ep element upstream of EGFP, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:EGFP-attR1-attR2

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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