We narrowed to 5,097 results for: puc
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Plasmid#205441PurposeLevel 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.DepositorInsertRepB gRNA
UseSynthetic BiologyAvailable SinceMay 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB8792
Plasmid#126910PurposeTemplate for PCR amplification of ADE2 gRNA biobrick for targeting Saccharomyces cerevisiae ADE2 geneDepositorInsertADE2 gRNA
UseSynthetic BiologyAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE8
Plasmid#79463PurposesgRNA shuttle vector; module 2EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyMutationBsaI site eliminatedAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH49
Plasmid#128211Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZH518
Plasmid#102671PurposeTetracycline inducible expression of GFPmut2 using autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVH613
Plasmid#169602PurposeTier-2 vector encoding PCREm4-driven Nluc and PmPGK1-driven Fluc expression (PCREm4-Nluc-pA::A2-pA::PmPGK1-Fluc-pA).DepositorInsertcAMP response element-controlled NanoLuc Luciferase expression cassette
ExpressionMammalianPromoterPCREm4 / PPGKAvailable SinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
ATM_N_FS_v2_Donor
Plasmid#214815PurposeN-terminal tagging of human ATM with 3xFLAG_Twin_StrepDepositorInsert3xFLAG_2xStrep for tagging ATM at the N-terminus (ATM Synthetic, Human)
UseCRISPRTags3xFLAG_2xStrepExpressionMammalianAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPotef-LN22*-3xGNLS-cbx
Plasmid#86780PurposeExpresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.DepositorInsertLN*
UseUstilago maydis expressionTagsGfpExpressionBacterialPromoterPotefAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pILGFPU0 (R)
Plasmid#83562PurposeExpress yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoterDepositorInsertTEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTW102
Plasmid#115945PurposeORF insert for replicon MoCloDepositorInsertLvl0 DDd-L7Ae
UseSynthetic BiologyAvailable SinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR540
Plasmid#207885Purposetemplate for 2 gRNA amplificationDepositorInsertscaffold-pU6
ExpressionBacterialAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS021
Plasmid#207917PurposeLevel 0 for gRNA in E2 module (5' CCAT-3' ACTA)DepositorInsertpU6-AarI-scaffold-term
UseCRISPRAvailable SinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKE013
Plasmid#207916PurposeLevel 0 for gRNA in E1 module (5' CTGC-3' CCAT)DepositorInsertpU3-AarI-scaffold-term
UseCRISPRAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFPQ4
Plasmid#83559PurposeUsed to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.DepositorInsertGAL7 promoter-yEGFP
ExpressionYeastPromoterGAL7Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
TC1092
Plasmid#164879PurposeCMV-dcas13d-NLS-ADARdd- mCherryDepositorInsertdcas13d-ADARdd
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZH509
Plasmid#102664PurposeTetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAT15001-AncBE4max
Plasmid#163016Purposeplasmid expressing AncBE4maxDepositorInsertAncBE4max
UseCRISPRTags3xFLAG and SV40 NLSExpressionMammalianAvailable SinceSept. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGE12
Plasmid#79469PurposesgRNA shuttle vector; module 5DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyMutationBsaI site eliminatedAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE13
Plasmid#79471PurposesgRNA shuttle vector; module 6DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyMutationBsaI site eliminatedAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE14
Plasmid#79473PurposesgRNA shuttle vector; module 7DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyMutationBsaI site eliminatedAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only