Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,334 results for: gateway

Showing: 8901 - 8950 of 18334 results

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pDONR223-ULK3

Plasmid

#23646

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ULK3 (ULK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pTripz NEO DEST

Plasmid

#209072

Purpose

Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest

Depositor

Matthew Oser

Article

Oser et al Cancer Discov. 2019 Feb;9(2):230-247. do

Type

Empty backbone

Use

Lentiviral; Gateway destination vector to be used…

Tags

3X FLAG

Promoter

CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP1 Delta RC

Plasmid

#164522

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 Delta RC (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PANK4

Plasmid

#23419

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PANK4 (PANK4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-SLC52A3_STOP

Plasmid

#161347

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC52A3 (C20orf54 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-PKDCC

Plasmid

#23387

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PKDCC (PKDCC Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NLK

Plasmid

#23642

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NLK (NLK Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-LETMD1

Plasmid

#132294

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

LETMD1 (LETMD1 Human)

Expression

Mammalian

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-PRKY

Plasmid

#23506

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKY (PRKY Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TIE1

Plasmid

#23946

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TIE1 (TIE1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PIM2

Plasmid

#23587

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIM2 (PIM2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-ANKH_STOP

Plasmid

#161444

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

ANKH (ANKH Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-VRK2

Plasmid

#23534

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

VRK2 (VRK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-LETMD1_STOP

Plasmid

#161460

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

LETMD1 (LETMD1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-VRK3

Plasmid

#23687

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

VRK3 (VRK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_9

Plasmid

#60258

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

NKX6-1 enhancer (NKX6-1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-RBKS

Plasmid

#23681

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RBKS (RBKS Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MYO3B

Plasmid

#23524

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MYO3B (MYO3B Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TESK2

Plasmid

#23695

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TESK2 (TESK2 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR207 SARS-CoV-2 NSP1 124A/125A

Plasmid

#164523

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 124A/125A (ORF1ab )

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-STK33

Plasmid

#23823

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK33 (STK33 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pENTR1A_mmFbxw7a (closed)

Plasmid

#160101

Purpose

Expresses murine Fbxw7 alpha in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7alpha (Fbxw7 Mouse)

Use

Gateway entry vector

Expression

Mammalian

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
JDW 823 (pDONR221-V5-mScarlet-I-hsKRAS4A-G12V)

Plasmid

#156406

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A with G12V mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12V (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Mutation

mutant KRAS4A-G12V

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-ACE2 receptor 19-615

Plasmid

#167012

Purpose

Gateway-compatible Entry vector encoding ACE2 receptor (aa19-615) interacting with spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Article

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

ACE2 (ACE2 Human)

Expression

Mammalian

Mutation

Amino acids 19-615, No stop codon

Available Since

March 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR223-PI4KAP2

Plasmid

#23601

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PI4KAP2 (PI4KAP2 Human)

Use

Gateway donor vector

Mutation

V154G

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)

Plasmid

#156405

Purpose

Gateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4A-WT (KRAS Human)

Use

Gateway cloning

Tags

mScarlet-I

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-FLJ40852

Plasmid

#23377

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FLJ40852 (FLJ40852 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PHKB

Plasmid

#23859

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PHKB (PHKB Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_10

Plasmid

#60259

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

RFX6 enhancer (RFX6 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-XYLB

Plasmid

#23639

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

XYLB (XYLB Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pENTR D-TOPO-C3_25

Plasmid

#60268

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ROCK1 enhancer (ROCK1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PION

Plasmid

#23369

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PION (PION Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-BMP2KL

Plasmid

#23399

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMP2KL (BMP2KL Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

Plasmid

#127343

Purpose

N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

Flag- HA- Tandem Epitopes

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

None

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCR8/GW_TOPO_Cdk13_Untagged

Plasmid

#127342

Purpose

Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Insco et al Science. 2023 Apr 21;380(6642):eabn7625.

Insert

Cyclin Dependent Kinase 13 (Cdk13 Mouse)

Use

Entry vector with transgene for gateway cloning

Mutation

Base pairs 1-1554 of transgene are codon optimize…

Promoter

None

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-Cavin4b_PRDmt

Plasmid

#176017

Purpose

Multisite gateway middle entry clone containing zebrafish Cavin4b_PRDmt with no stop codon. Parton lab clone JLY.

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

Cavin4b (PRDmt) (cavin4b Zebrafish)

Use

Gateway entry clone

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-BIN1_tv8

Plasmid

#126574

Purpose

Multisite gateway middle entry vector containing human BIN1 transcriptional variant 8 (muscle specific) with no ATG. Parton lab clone DVA.

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

BIN1 (BIN1 Human)

Use

Gateway entry clone

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR221-MAGT1_STOP

Plasmid

#161468

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MAGT1 (MAGT1 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pENTR1A_mmFbxw7_R505C (closed)

Plasmid

#160103

Purpose

Expresses murine Fbxw7 alpha with the R505C loss-of-function mutation in mammalian cells

Depositor

Rizwan Haq

Article

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

Fbxw7 alpha R505C (Fbxw7 Mouse)

Use

Gateway entry vector

Expression

Mammalian

Mutation

R505C

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C3_7

Plasmid

#60256

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC30A8 enhancer (SLC30A8 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C3_17

Plasmid

#60263

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZFAND3 enhancer (ZFAND3 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C3_18

Plasmid

#60264

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZBED3 enhancer (ZBED3 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C3_19

Plasmid

#60265

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

THADA enhancer (THADA Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C3_22

Plasmid

#60267

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

ZMIZ1 enhancer (ZMIZ1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C3_26

Plasmid

#60269

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

OBFC2A enhancer (NABP1 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C3_31

Plasmid

#60273

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SLC2A2 enhancer (SLC2A2 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C3_34

Plasmid

#60276

Purpose

This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

PNMA2 enhancer (PNMA2 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C2_11

Plasmid

#60251

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SMYD3 inactive enhancer (SMYD3 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR D-TOPO-C2_13

Plasmid

#60252

Purpose

This plasmid contains a human pancreatic islet inactive enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

Depositor

Jorge Ferrer

Article

Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

Insert

SPATA19 inactive enhancer (SPATA19 Human)

Use

Entry vector

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PANK3

Plasmid

#23384

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PANK3 (PANK3 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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