We narrowed to 39,968 results for: lat

1. pCDH_TNF-SBP-EGFP

   Plasmid

   #65282

   Purpose

   TNF reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Lentiviral

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Promoter

   CMV

   Available Since

   June 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. PPIB-bio-His

   Plasmid

   #53413

   Purpose

   Expresses full-length Peptidyl-prolyl cis-trans isomerase B precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PPIB (PPIB Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCK340

   Plasmid

   #192639

   Purpose

   Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Inserts

   Sp.pCas9-dSpG
   BBa_J23107-MCP-SoxS(R93A, S101A)
   

   Use

   CRISPR and Synthetic Biology

   Mutation

   SoxS has R93A and S101A mutations and dSpG has se…

   Promoter

   BBa_J23107 and Sp.pCas9

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. R702-X31-566: His6-MBP-tev-G-Hs.PDE6D(2-150)

   Plasmid

   #159689

   Purpose

   E. coli protein expression of His6-MBP-tev-G-Hs.PDE6D(2-150)

   Depositor

   Dominic Esposito

   Article

   Dharmaiah et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

   Insert

   His6-MBP-tev-G-Hs.PDE6D(2-150) (PDE6D Human)
   

   Tags

   His6-MBP-tev

   Expression

   Bacterial

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR8/GW_TOPO_Cdk12_Untagged

   Plasmid

   #127176

   Purpose

   Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

   Depositor

   Phil Sharp

   Article

   Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

   Insert

   Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
   

   Use

   Entry vector with transgene for gateway cloning

   Tags

   None

   Promoter

   None

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSF4 CMV 5TOP intron renilla TRICK CTE polyA

   Plasmid

   #64544

   Purpose

   5′ TOP TRICK reporter mRNA

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   12x PP7 stem-loops
   

   Tags

   24xMS2 stem loops, 5' TOP (5′ terminal oligo…

   Expression

   Mammalian

   Promoter

   Tetracycline-inducible promoter and 5′ UTR of hum…

   Available Since

   May 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO-Intron-CterFlag-MePCE

   Plasmid

   #113549

   Purpose

   Mammalian expression plasmid that can be used with the Flp-In T-REx system.

   Depositor

   Blerta Xhemalce

   Article

   Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

   Insert

   MePCE (MEPCE Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. sh-SRF

   Plasmid

   #100797

   Purpose

   expression of shRNA targeting SRF

   Depositor

   Joseph Gordon

   Article

   Mughal et al Cell Death Dis. 2015 Oct 29;6:e1944. doi

   Insert

   rat SRF
   

   Use

   RNAi

   Promoter

   H1

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-MCS-eDHFR(69K6)-EGFP

   Plasmid

   #172101

   Purpose

   Mammalian expression of a protein of interest fused to the N-terminus of eDHFR(69K6)-EGFP

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   22aa-eDHFR(69K6)-EGFP
   

   Expression

   Mammalian

   Mutation

   eDHFR(69K6): Hexalysine (K6) sequence is inserted…

   Promoter

   CMV

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTH732-2µ-RLuc/staCFLuc

    Plasmid

    #40607

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTET2-lucCP-3MS2

    Plasmid

    #198351

    Purpose

    Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Firefly Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    pTET2

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.2/NS5-YFP-NLS-mut

    Plasmid

    #120832

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    ZIKV NS5-eYFP "NLS-mut"
    

    Tags

    eYFP-V5

    Expression

    Mammalian

    Mutation

    R372A/Q373A/K387A/H388A/K389A

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. eSpCas9-EF-5Nanog_2

    Plasmid

    #174871

    Purpose

    CRISPR vector for generating Nanog STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic)
    

    Use

    CRISPR

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. VP-ARF

    Plasmid

    #89122

    Purpose

    mammalian expression of p14-hARF linked to VP16 activation domain

    Depositor

    Elizabeth Wilson

    Article

    Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

    Insert

    p14-hARF (CDKN2A Human)
    

    Tags

    VP16

    Expression

    Mammalian

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)- e18 LOF

    Plasmid

    #131729

    Purpose

    Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJZC33

    Plasmid

    #62330

    Purpose

    sgRNA + 2x MS2 with MCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2 binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX neo PFKP-GFP K281R

    Plasmid

    #138288

    Purpose

    Mammalian Expression, Lentiviral. Expression of human phosphofructokinase platelet isoform PFKP-GFP fusion protein with K281R mutation

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    PFKP (PFKP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K281R

    Promoter

    CMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR KIF17 (1-738)

    Plasmid

    #60406

    Purpose

    Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    KIF17 (KIF17 Human)
    

    Use

    Lentiviral

    Tags

    GB1, Strep-Tag, and sfGFP

    Expression

    Mammalian

    Mutation

    Truncated version amino acids 1-738

    Promoter

    SFFV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH_TNF-SBP-mCherry

    Plasmid

    #65283

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQFlag-USP11 CS puroR

    Plasmid

    #46748

    Purpose

    Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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