We narrowed to 14,886 results for: pET

1. pET11+PCNA3-CYP119(T214V)

   Plasmid

   #66137

   Purpose

   Expresses the T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

   Insert

   CYP119 (cyp119 )
   

   Use

   Tags

   Sulfolobus solfataricus PCNA3

   Expression

   Bacterial

   Mutation

   T214V

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15b+PCNA1 G108C/P186C

   Plasmid

   #66174

   Purpose

   Expresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   G108C, P186C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-p53 (Guinea Pig 1-88)

   Plasmid

   #62059

   Purpose

   Expression of Guinea Pig p53 (residues 1-88) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

   Insert

   p53
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-88

   Promoter

   T7

   Available since

   Jan. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET-20b-T7-Ntx-K49

   Plasmid

   #58715

   Purpose

   Expresses in Escherichia coli T7-Notexin with the amino acid substitution D49K

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Use

   Tags

   T7 peptide tag

   Expression

   Bacterial

   Mutation

   Changed Aspartic Acid 49 to Lysine

   Promoter

   T7

   Available since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b-EC12-129/138Avi

   Plasmid

   #47927

   Purpose

   Expresses E-cadherin EC12 with C9A, K129C/D138C mutations and avi tag in E.Coli.

   Depositor

   Tanja Kortemme

   Article

   Ritterson et al J Am Chem Soc. 2013 Aug 7.

   Insert

   E-Cadherin EC12
   

   Use

   Tags

   6xHis, Avi Tag, and TEV Cleavage

   Expression

   Bacterial

   Mutation

   Mutations C9A, K129C, D138C

   Promoter

   T7

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET12a-AL-103 Y32F Y96F

   Plasmid

   #47092

   Depositor

   Marina Ramirez-Alvarado

   Article

   DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

   Insert

   AL-103 Y32F Y96F (IGKV3-20 Human)
   

   Use

   Tags

   ompT

   Expression

   Bacterial

   Mutation

   Y32F Y96F

   Promoter

   T7

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET21b-Tipin-His-L195A

   Plasmid

   #23123

   Depositor

   Aziz Sancar

   Article

   Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

   Insert

   Tipin (TIPIN Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Changed Leucine 195 to Alanine

   Promoter

   Available since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

8. HI0058/pET-21a(+)c-tag

   Plasmid

   #13097

   Depositor

   SeWon Suh

   Article

   Ku et al Acta Crystallogr D Biol Crystallogr. 200

   Insert

   3-deoxy-manno-octulosonate cytidylyltransferase (kdsB Haemophilus influenzae)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-21a(+) SurE D8A

   Plasmid

   #12685

   Depositor

   SeWon Suh

   Article

   Lee et al Nat Struct Biol. 2001 Sep . 8(9):789-94.

   Insert

   SurE (TM1662 )
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Aspartate 8 to Alanine

   Promoter

   Available since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

10. Pet28a His6-HSPB7 (zebrafish) WT

    Plasmid

    #108917

    Purpose

    Express WT Zebrafish His6-HSPB7 in bacteria

    Depositor

    Yimon Aye

    Article

    Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

    Insert

    His6-HSPB7 (hspb7 Zebrafish)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-PKAsub(T/A)-YPet-NES

    Plasmid

    #138205

    Purpose

    Encodes negative-control substrate fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub(T/A)-YPet-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-PKAsub-YPet-Kras

    Plasmid

    #138206

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub-YPet-Kras
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-SdrG_N2N3-B1-B2-HIS-ybbr

    Plasmid

    #117979

    Purpose

    SdrG N2 and N3, B1 and B2 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_N2N3-B1-B2
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-SdrG_N2N3-B1-HIS-ybbr

    Plasmid

    #117980

    Purpose

    SdrG N2 and N3, B1 domains with C-terminal ybbr tag for covalent immobilization

    Depositor

    Hermann Gaub

    Article

    Milles et al Nat Commun. 2018 Nov 12;9(1):4764. doi:

    Insert

    SdrG_N2N3-B1
    

    Use

    Tags

    H3V 3C, HIS, and ybbr

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET His10-Draper INT

    Plasmid

    #126670

    Purpose

    Expression of His10-Draper intracellular domain for purification from E. coli

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper cytoplasmic domain
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. Sfp pet29b C-terminal His Tag

    Plasmid

    #75015

    Purpose

    Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

    Depositor

    Michael Burkart

    Article

    Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

    Insert

    Sfp
    

    Use

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. human KANK2 Ankyrin Repeats pET151

    Plasmid

    #164054

    Purpose

    Expresses human KANK2 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Use

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET29-bdSUMO-Anti-ALFA nanobody

    Plasmid

    #212311

    Purpose

    Expression of anti-ALFA nanobody

    Depositor

    Arthur Laganowsky

    Article

    Stover et al J Struct Biol X. 2024 Feb 2;9:100097. do

    Insert

    anti-ALFA nanobody
    

    Use

    Tags

    bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET51b-His-TEV-SNAP-tag

    Plasmid

    #167269

    Purpose

    Overexpression of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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