We narrowed to 505 results for: SPY

1. dSpyCas9-E566-GS-LOV2-VPR

   Plasmid

   #231576

   Purpose

   Potent light-deactivated CRISPRa

   Depositor

   Dominik Niopek

   Article

   Wolf et al bioRxiv 2024.12.04.626757

   Insert

   dSpyCas9-VPR with an AsLOV2 insertion behind E566
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR-Spy-CD28(CD45TM)

   Plasmid

   #223624

   Purpose

   Lentiviral expression of human CD28 (CD45TM) with SpyTag003 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   Spytag003

   Expression

   Mutation

   CD28 transmembrane domain replaced by CD45 transm…

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-Spy-CD28(Y191F)

   Plasmid

   #223623

   Purpose

   Lentiviral expression of human CD28 (Y191F) with SpyTag003 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   Spytag003

   Expression

   Mutation

   Y191F

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-H2-18 Heavy chain-SpyTag003

   Plasmid

   #216307

   Purpose

   Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   H2-18 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SnoopTag-AffiHER2-SpyTag

   Plasmid

   #216280

   Purpose

   Expresses an affibody to HER2 fused to SnoopTag and SpyTag, for bacterial expression

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SnoopTag-AffiHER2-SpyTag
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6, SnoopTag, SpyTag, and Thrombin cleavage sit…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SnoopTag-SpyTag-(AffiHER2)x3

   Plasmid

   #216281

   Purpose

   Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SnoopTag-SpyTag-(AffiHER2)x3
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6, SnoopTag, and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyTag-RBD Omicron BQ.1.1

   Plasmid

   #214725

   Purpose

   Expresses SARS-CoV-2 Omicron BQ.1.1 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

   Insert

   Receptor-Binding Domain (S SARS-CoV-2)
   

   Use

   Tags

   SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST14-Cys-SpyCatcher (Min)

   Plasmid

   #216713

   Purpose

   Expresses truncated minimal form of SpyCatcher with free cysteine for labeling in bacteria. See also Li...Howarth, J Mol. Biol., 2014

   Depositor

   Alex Dunn

   Article

   Zhong et al Cell Rep Methods. 2022 Sep 2;2(9):100278

   Insert

   Cys-SpyCatcher (Min)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   See Li...Howarth, J. Mol. Biol., 2014

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZa pLtetO CsgA-SpyTag

   Plasmid

   #204503

   Purpose

   Bacterial expression of CsgA with C-terminal SpyTag peptide and GS linker

   Depositor

   Urartu Seker

   Article

   Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

   Insert

   CsgA
   

   Use

   Synthetic Biology

   Tags

   6xHis, SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   pLtetO

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFN-HaloTag-TalinR7R8-SpyTag

    Plasmid

    #192046

    Purpose

    Plasmid expresses human talin R7R8 domain flanked between a HaloTag and a SpyTag

    Depositor

    Ionel Popa

    Article

    Dahal et al Sci Adv. 2022 Jul 15;8(28):eabl7719. doi

    Insert

    TLN1 (TLN1 Human)
    

    Use

    Tags

    HaloTag and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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