Addgene

We narrowed to 1,192 results for: 5-Oct

Showing: 511 - 540 of 1192 results

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pJWK_VD_03

Plasmid

#158104

Purpose

CaV1.2 5'UTR luciferase reporter

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR of CACNA1C (uc009zdu.1)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pMM765

Plasmid

#133602

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM766

Plasmid

#133603

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD6

Plasmid

#73915

Purpose

tissue-specific multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

:tRNA:gRNA:tRNA:gRNA:tRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

UAS-DSCP

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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p5E-slc1a3b

Plasmid

#170205

Purpose

Gateway 5’ entry vector with the slc1a3b promoter

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

slc1a3b (slc1a3b )

Use

Unspecified; Zebrafish

Tags

Expression

Mutation

Promoter

slc1a3b

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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GST-PRMT2

Plasmid

#34650

Depositor

Steven Clarke

Article

Miranda et al Biochem Biophys Res Commun. 2005 Oct 28;

Insert

PRMT2 (PRMT2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pCFD7

Plasmid

#73916

Purpose

gRNA vector for AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

tRNA:hAsCpf1-gRNA:tRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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GalT-moxMaple3

Plasmid

#120884

Purpose

expression of moxMaple3 localized to the Golgi

Depositor

Erik Snapp

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Insert

moxMaple3

Use

Tags

GalT signal anchor transmembrane domain

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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act-AsCpf1

Plasmid

#73917

Purpose

expression plasmid of AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

hAsCpf1

Use

Tags

NLS-3xHA

Expression

Insect

Mutation

Promoter

act5C

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSG 12-6

Plasmid

#63870

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

UAG near 5' end

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG 12DP

Plasmid

#63876

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

-1 frameshift near 5' end

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pSG lacZ

Plasmid

#63874

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

+1 frameshift near 5' end

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pAW-SpyCsn2

Plasmid

#113251

Purpose

E. coli expression vector for S. pyogenes Csn2-MBP-His

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Csn2

Use

Tags

MBP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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Ube2d1

Plasmid

#31409

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D1 Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pAW-SpyCas1

Plasmid

#113248

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAW-SpyCas2

Plasmid

#113249

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas2

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas2

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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Ube2d2

Plasmid

#31410

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D2 Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDL14

Plasmid

#17837

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

gpdA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Tags

Expression

Mutation

Promoter

Available since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
Human Mevalonate Kinase (MK)

Plasmid

#52429

Purpose

bacterial expression of human Mevalonate Kinase (MK)

Depositor

Henry Miziorko

Article

Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

Insert

Human Mevalonate Kinase (MVK Human)

Use

Tags

Expression

Bacterial

Mutation

see comments

Promoter

T7

Available since

April 10, 2014

Availability

Academic Institutions and Nonprofits only
pISH-Trpm5

Plasmid

#105993

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Trpm5 (Trpm5 Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSG lac10

Plasmid

#63875

Purpose

Measurements of translational accuracy

Depositor

Albert Dahlberg

Article

O'Connor et al Proc Natl Acad Sci U S A. 1993 Oct 1;90(

Insert

lacZ (lacZ Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

-1 frameshift near 5' end

Promoter

PtacI

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pDL13

Plasmid

#17836

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Tags

Expression

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
Ube2d3

Plasmid

#31411

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D3 Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pDL12c

Plasmid

#17835

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

gpdA promoter, lacZ, argB_1626,

Use

Synthetic Biology; Aspergillus nidulans expression

Tags

Expression

Mutation

The argB_1626 mutation consists of two sequential…

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
p64-101

Plasmid

#25911

Depositor

Kenneth Marcu

Article

Stanton et al Nucleic Acids Res. 1982 Oct 11. 10(19):5

Insert

Ig germline gamma-1 switch region alpha

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 11, 2010

Availability

Academic Institutions and Nonprofits only
pAW092

Plasmid

#113250

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1 H205A

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

H205A

Promoter

T7

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Use

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHisAlix deltaPRD

Plasmid

#42577

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

delta-PRD ALIX (residues 1-702) (PDCD6IP Human)

Use

Tags

HIS-TEV

Expression

Bacterial

Mutation

silent mutation A1617G (numbering in ALIX gene)

Promoter

Available since

Dec. 19, 2013

Availability

Academic Institutions and Nonprofits only
pDONOR-PARK6e5-I368N

Plasmid

#86154

Purpose

Donor plasmid for PARK6 exon5 I368N sequence. Also contains TagBFP and dTomato

Depositor

Jens Schwamborn

Article

Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

Insert

PINK1 exon 5 homology arms (PINK1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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sg_shuttle_RFP657

Plasmid

#134968

Purpose

Lentiviral vector for sgRNA expression

Depositor

Beat Bornhauser

Article

Huang et al Hemasphere. 2020 Sep 30;4(5):e456. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6-sgRNA; EFS-RFP657

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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