We narrowed to 1,198 results for: e.coli
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Plasmid#227360PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin T103A (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin L102K
Plasmid#227359PurposeFor bacterial expression of parkin with a mutated ACT.DepositorInsertParkin L102K (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin M458L
Plasmid#227356PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin M458L (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin R256C
Plasmid#227355PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin R256C (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin R234Q
Plasmid#227354PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin R234Q (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin V224A
Plasmid#227353PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin V224A (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin P37L
Plasmid#227352PurposeFor bacterial expression of hyperactive parkin mutant.DepositorInsertParkin P37L (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression. Chan…Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ApyADO
Plasmid#226248PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyODepositorInsertsHis6_ApyA
ApyD
ApyO
TagsHexa-HistidineExpressionBacterialAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ApyAO
Plasmid#226242PurposeProduction of ApyA peptide modified by the cytochrome P450 enzyme ApyODepositorInsertsHis6_ApyA
ApyO
TagsHexa-HistidineExpressionBacterialAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_ApyAD
Plasmid#226241PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyDDepositorInsertsHis6_ApyA
ApyD
TagsHexa-HistidineExpressionBacterialAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-dCas9-FCPF
Plasmid#220133PurposeExpresses dCas9-FCPF in E.coliDepositorInsertdCas9-FCPF (cas9 Synthetic, S. pyogenes)
Tags6xHisExpressionBacterialMutationFCPF sequence inserted on C-terminalPromoterT7Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
TagsTEV cleavable His10ExpressionBacterialPromoteraraBADAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2061
Plasmid#186299PurposeExpresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoterDepositorInsertsLexA-HBD-B112
Cas9
UseCRISPR and Synthetic BiologyExpressionBacterial and YeastPromoter2xLexop-minimalCyc1 and PACT1Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R1CiM_4EGFP_R3EGFPi2
Plasmid#185968PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.DepositorInsertMolecular IMPLY gate
UseSynthetic BiologyAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R3tdTomatoi2
Plasmid#185969PurposeExpresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.DepositorInsertPr-i2rbs-tdTomato cassette in reverse direction in Network Brick
UseSynthetic BiologyPromoterPRAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-UTX(420-633)-H6
Plasmid#183773PurposeExpress pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.DepositorInsertUTX
Tags6 His tag at the C-terminusExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
DT033A
Plasmid#159394PurposeConstitutive murine mature BDNF expression, controlled under E.coli tac promoterDepositorInsertpr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)
UseSynthetic BiologyTagsFLAGExpressionBacterialPromotertac promoterAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_3H1L_02.395
Plasmid#155198PurposeExpresses 3H1L_02.395 in E.coliDepositorInsert3H1L_02.395
Tags6x His TagExpressionBacterialAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97E
Plasmid#125825PurposeExpresses GST-RPN13 (19-132) K97E in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged lysine 97 to glutamatePromoterT7Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K83A
Plasmid#125829PurposeExpresses GST-RPN13 (19-132) K83A in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged lysine 83 to alaninePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97A
Plasmid#125823PurposeExpresses GST-RPN13 (19-132) K97A in E.coliDepositorAvailable SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97D
Plasmid#125824PurposeExpresses GST-RPN13 (19-132) K97D in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged lysine 97 to aspartatePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104A
Plasmid#125826PurposeExpresses GST-RPN13 (19-132) R104A in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged arginine 104 to alaninePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104D
Plasmid#125827PurposeExpresses GST-RPN13 (19-132) R104D in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged arginine 104 to aspartatePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104E
Plasmid#125828PurposeExpresses GST-RPN13 (19-132) R104E in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged arginine 104 to glutamatePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-C-SH2
Plasmid#111419Purposeexpress murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
TagsHHHHHHENLYFQGExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-953)
Plasmid#73743PurposeExpression of GFP-PP-RPN2 (918-53) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (932-953)
Plasmid#73745PurposeExpression of GFP-PP-RPN2 (932-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 932-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-947)
Plasmid#73747PurposeExpression of GFP-PP-RPN2 (918-947) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-947 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (797-953)
Plasmid#74228Purpose[Edit] Expression of GFP-PP-RPN2 (797-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)(M148D/F149D)
Plasmid#71388PurposeExpression of hUCH37 (M148D/F149D) in E.coliDepositorInserthUCH37 (ISF1) M148D/F149D (UCHL5 Human)
TagsHIS-TEV sequenceExpressionBacterialMutationchanged methionine 148 to aspartate, phenylalanin…PromoterT7Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 DM
Plasmid#61931PurposeExpression of hUCH37 (ISF1) M269E/N291D in E.coliDepositorInserthUCH37 (ISF1) M269E/N291D (UCHL5 Human)
Tags6x HISExpressionBacterialMutationChanged methionine 269 to glutamate and asparagin…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET151-hUCH37 (ISF1)(M148A/F149A)
Plasmid#61933PurposeExpression of hUCH37 (M148A/F149A) in E.coliDepositorInserthUCH37 (ISF1) (UCHL5 Human)
Tags6x HISExpressionBacterialMutationMethionine 148 and Phenylalanine 149 were mutated…PromoterT7Available SinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRa34_3302
Plasmid#49694DepositorInsertECF34_3302
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa33_375
Plasmid#49691DepositorInsertECF33_375
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa32_3724
Plasmid#49690DepositorInsertECF32_3724
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa31_34
Plasmid#49687DepositorInsertECF31_34
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa30_35
Plasmid#49685DepositorInsertECF30_35
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa27_1331
Plasmid#49681DepositorInsertECF27_1331
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa26_4464
Plasmid#49680DepositorInsertECF26_4464
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa26_837
Plasmid#49679DepositorInsertECF26_837
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa25_1645
Plasmid#49678DepositorInsertECF25_1645
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa25_1643
Plasmid#49677DepositorInsertECF25_1643
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa22_1147
Plasmid#49675DepositorInsertECF22_1147
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa17_1458
Plasmid#49671DepositorInsertECF17_1458
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc31_34
Plasmid#49747DepositorInsertAS31_34
UseSynthetic BiologyExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc30_35
Plasmid#49746DepositorInsertAS30_35
UseSynthetic BiologyExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRc22_1147
Plasmid#49740DepositorInsertAS22_1147
UseSynthetic BiologyExpressionBacterialMutationCodon optimized for E.coliPromoterpLuxAvailable SinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVRa14_1324
Plasmid#49665DepositorInsertECF14_1324
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable SinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only