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p16-1 hTRF2

Plasmid

#12299

Depositor

Titia de Lange

Article

Broccoli et al Nat Genet. 1997 Oct . 17(2):231-5.

Insert

hTRF2 (TERF2 Human)

Use

Tags

Expression

Bacterial

Mutation

A434 deleted; There is a stop codon in the 5'…

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
pMT-GFP-Pat coiled-coil (cc)

Plasmid

#31757

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Patronin (Patronin Fly)

Use

Tags

GFP

Expression

Insect

Mutation

Contains only the coiled-coil domain: aa 535-1457

Promoter

Metallothionein

Available since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pDONR207 SARS-CoV-2 NSP1 Delta RC

Plasmid

#164522

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions K164A/H165A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 Delta RC (ORF1ab )

Use

Tags

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Promoter

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR207 SARS-CoV-2 NSP1 124A/125A

Plasmid

#164523

Purpose

Gateway-compatible Entry vector, with insert of mutated NSP1 CDS from SARS-CoV-2 isolate Wuhan-Hu-1 (amino acid substitutions R124A/K125A)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Insert

NSP1 124A/125A (ORF1ab )

Use

Tags

Expression

Mammalian

Mutation

Insert of NSP1 gene's CDS from SARS-CoV-2 is…

Promoter

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MUC2 D1 with cleavable His tag

Plasmid

#181813

Purpose

Expresses human MUC2 D1 with cleavable His tag, residues 21-389

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc2 D1 (MUC2 Human)

Use

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pFM038

Plasmid

#40141

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R183-5A + 7SA; base pairs 6-523 of par-2 were re…

Promoter

pie-1

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM041

Plasmid

#40144

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (S241A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

S241A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM039

Plasmid

#40142

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R183-5A + S241A; base pairs 6-523 of par-2 were …

Promoter

pie-1

Available since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only
pFM036

Plasmid

#40139

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (K162A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

K162A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pFM052

Plasmid

#40146

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (C56S) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

C56S; base pairs 6-523 of par-2 were recoded to …

Promoter

pie-1

Available since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
pFM037

Plasmid

#40140

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (R163A) (par-2 Nematode)

Use

Gateway destination vector

Tags

GFP

Expression

Worm

Mutation

R163A; base pairs 6-523 of par-2 were recoded to…

Promoter

pie-1

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00181

Plasmid

#34334

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5393 (apt Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
DsRed-p150 217–548

Plasmid

#51146

Purpose

Inhibits dynein-dynactin interaction in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 2002 Oct 28;159(2):245-54.

Insert

DCTN1 (DCTN1 Chicken)

Use

Tags

Expression

Mammalian

Mutation

Q118R, E340K

Promoter

CMV

Available since

March 28, 2017

Availability

Academic Institutions and Nonprofits only
pFLAG-ZYG11B

Plasmid

#110550

Purpose

expresses a FLAG-tagged human ZYG11B protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11B (ZYG11B Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pFLAG-ZYG11A

Plasmid

#110551

Purpose

expresses a FLAG-tagged human ZYG11A protein in mamalian cells

Depositor

Edward Kipreos

Article

Balachandran et al J Cell Biol. 2016 Oct 24;215(2):151-166.

Insert

ZYG11A (ZYG11A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFGC5941-PacI-ath-STTM2937

Plasmid

#84112

Purpose

target miRNA2937 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM2937

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM159

Plasmid

#84084

Purpose

target miRNA159 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM159

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941DR5-PacI-ath-STTM390

Plasmid

#84117

Purpose

target miRNA390 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM390

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM858a/b

Plasmid

#84177

Purpose

target miRNA858 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM858a/b

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI-ath-STTM828

Plasmid

#84157

Purpose

target miRNA828 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM828

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only