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Plasmid#173639PurposeExpresses a Keap1-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Keap1 (Keap1 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgBap1#1/Cre
Plasmid#173581PurposeExpresses a Bap1-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting Bap1 (Bap1 Mouse)
UseCRISPR, Cre/Lox, and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Cdk2ap1ΔN (MT2B2)
Plasmid#178034PurposeExpression vector for the purpose of protein purification.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseNonviralTags6xHIS - Thrombin - MBP- TEV-TRSExpressionBacterialMutationFirst N-Terminal 27aa are deletedPromoterT7LacIAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCheck Cdk2ap1ΔN (MT2B2)
Plasmid#178029Purposeexpression vector to test translation efficiency by luciferase reporter.DepositorInsertCdk2ap1 (Cdk2ap1 Mouse)
UseLuciferase; NonviralTagsLuciferaseExpressionMammalianMutationPromoterCloned inAvailable sinceJan. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTHAP12-N1.1.2-gDNA
Plasmid#175860PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertTHAP12-Nickase2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSin4 UAP1 F383G
Plasmid#169891PurposeUsed for generation of stable mammalian cell lines expressing UAP1 with with F383G mutationDepositorInsertUDP-N-acetylhexosamine pyrophosphorylase (UAP1 Human)
UseLentiviralTagsNoneExpressionMammalianMutationChanged Phenylalanine (F) at position 383 to glyc…PromoterAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E7-D192D
Plasmid#154024PurposeBichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)DepositorInsertBAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationBAP1 c.576C>T, p.D192DPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
RG6-BAP1_E11-G312G
Plasmid#154026PurposeBichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)DepositorInsertBAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
UseSplicing reporterTagsDsRed1, FLAG, SV40 NLS, and eGFPExpressionMammalianMutationBAP1 c.936T>G, p.G312GPromoterAvailable sinceFeb. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-LAP1-mCherry
Plasmid#159525PurposeExpresses mCherry from LAP1 promoterDepositorInsertmCherry
UseAAVTagsExpressionMammalianMutationPromoterLAP1Available sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-MAPKAP1RBD
Plasmid#120710PurposeExpresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus productionDepositorInsertMAPKAP1 RBD
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationAmino acids 279-353PromoterCMVAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ARHGAP12 WW domain #1
Plasmid#104274PurposeBacterial expression of WW domain #1 from ARHGAP12DepositorInsertARHGAP12 WW domain #1 (ARHGAP12 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 ARHGAP12 WW domain #2
Plasmid#104293PurposeBacterial expression of WW domain #2 from ARHGAP12DepositorInsertARHGAP12 WW domain #2 (ARHGAP12 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
RANGAP1 G6.4 gRNA
Plasmid#90873Purpose3rd generation lentiviral gRNA plasmid targeting human RANGAP1DepositorInsertRANGAP1 (Guide Designation G6.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
RANGAP1 G5.4 gRNA
Plasmid#90872Purpose3rd generation lentiviral gRNA plasmid targeting human RANGAP1DepositorInsertRANGAP1 (Guide Designation G5.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rad51AP1 B11.3 gRNA
Plasmid#90868Purpose3rd generation lentiviral gRNA plasmid targeting human Rad51AP1DepositorInsertRad51AP1 (Guide Designation B11.3)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
RACGAP1 E8.4 gRNA
Plasmid#90865Purpose3rd generation lentiviral gRNA plasmid targeting human RACGAP1DepositorInsertRACGAP1 (Guide Designation E8.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
RACGAP1 E7.4 gRNA
Plasmid#90864Purpose3rd generation lentiviral gRNA plasmid targeting human RACGAP1DepositorInsertRACGAP1 (Guide Designation E7.4)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAMSAP1 E4.5 gRNA
Plasmid#90558Purpose3rd generation lentiviral gRNA plasmid targeting human CAMSAP1DepositorInsertCAMSAP1 (Guide Designation E4.5)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
CAMSAP1 E5.5 gRNA
Plasmid#90559Purpose3rd generation lentiviral gRNA plasmid targeting human CAMSAP1DepositorInsertCAMSAP1 (Guide Designation E5.5)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aP1Egfp
Plasmid#90167Purposecontains tmem88a specific gene promoter driving expression of eGFPDepositorInserttmem88aP1Egfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only