We narrowed to 2,241 results for: LOB;

1. pInt_attP1_chlorR

   Plasmid

   #205295

   Purpose

   Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pInt_attP1_ampR

   Plasmid

   #205296

   Purpose

   Integrating plasmid for ORBIT in E. coli. Standard attP site (#1).

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pInt_attP1_sacB_kanR

   Plasmid

   #205301

   Purpose

   Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Sperm whale Mb 64Y68F in pVP80K

   Plasmid

   #86956

   Purpose

   Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

   Depositor

   John Olson

   Insert

   Mb 64Y68F
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   64Y68F

   Promoter

   T5

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pInt_attP1_LCS_kanR

   Plasmid

   #205300

   Purpose

   Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pInt_attP1_tsXis_sacB_kanR

   Plasmid

   #205302

   Purpose

   Integrating plasmid for ORBIT in E. coli with self excising factor (Xis) and sacB for counter selection. Used for markerless modifications.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKP515

   Plasmid

   #159597

   Purpose

   Express E.coli FepA protein

   Depositor

   Kathleen Postle

   Article

   Vakharia et al J Bacteriol. 2002 Oct;184(19):5508-12. d

   Insert

   fepA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

   Plasmid

   #203349

   Purpose

   Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Molly Stevens, Christopher Grigsby

   Insert

   mGreenLantern
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Expression

   Mutation

   Promoter

   T7

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHelper_NoMutL_V2_gentR

   Plasmid

   #205293

   Purpose

   Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

   Depositor

   Scott Saunders

   Article

   Saunders et al bioRxiv 2023

   Insert

   CspRecT / Bxb-1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHelper_Ec1_V1_gentR

    Plasmid

    #205291

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHelper_Ec1_V1_ampR

    Plasmid

    #205292

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSP64TS (DM#117)

    Plasmid

    #15034

    Depositor

    Douglas Melton

    Article

    Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

    Insert

    beta-globin 5' and 3' UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pAIP4_4NFB

    Plasmid

    #234161

    Purpose

    Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    4NFB (PILRA Human)
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCT03

    Plasmid

    #212150

    Purpose

    Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.

    Depositor

    Kang Zhou

    Insert

    alanine dehydrogenase
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    PT7

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLL1271

    Plasmid

    #129282

    Purpose

    Expression plasmid with pLL1270 as backbone and alpha-Xylp(Ga0256695_ 1211)

    Depositor

    Lee Lynd

    Insert

    alpha-Xylp_1211 (Ga0256695_ 1211)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Clostridium thermocellum DSM1313 Cellobiose Phosp…

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Rosa26 mT/mG

    Plasmid

    #17787

    Depositor

    Liqun Luo

    Article

    Muzumdar et al Genesis. 2007 Sep . 45(9):593-605.

    Insert

    Rosa26 mT/mG
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    membrane tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

    Plasmid

    #203348

    Purpose

    Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

    Depositor

    Molly Stevens, Christopher Grigsby

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Template for t7-mediated in vitro transcription o…

    Tags

    Flanked by 5' and 3' UTRs from Xenopus …

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHelper_TS_V2_ampR

    Plasmid

    #214467

    Purpose

    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Insert

    CspRecT / Bxb-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    XylS / AraC

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

    Plasmid

    #203350

    Purpose

    Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

    Depositor

    Molly Stevens, Christopher Grigsby

    Insert

    Nano Luciferase
    

    Use

    Template for t7-mediated in vitro transcription o…

    Tags

    Flanked by 5' and 3' UTRs from Xenopus …

    Expression

    Mutation

    Promoter

    T7

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSP64T (DM#111)

    Plasmid

    #15030

    Depositor

    Douglas Melton

    Article

    Krieg et al Nucleic Acids Res. 1984 Sep 25. 12(18):7

    Insert

    beta-globin 5' and 3' UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge