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CoV2-N-S202R

Plasmid

#177950

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S202R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (S202R) (N )

Expression

Mammalian

Mutation

S202R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ827

Plasmid

#155321

Purpose

Constitutive expression of E-PIF6(1-100) in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-E-PIF6(1-100)-NLS-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pROF051

Plasmid

#155328

Purpose

Constitutive expression of SRDX-EL222 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-SRDX-NLS-EL222-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CoV2-N-P199L

Plasmid

#177949

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P199L) (N )

Expression

Mammalian

Mutation

P199L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pMZ828

Plasmid

#155322

Purpose

Constitutive expression of PhyB(1-650)-VP16 in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

Plasmid

#194217

Purpose

Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

UAS-ybTATA-tagBFP; PGK-mCitrine

Use

Lentiviral

Promoter

GAL4 UAS-ybTATA; PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

Plasmid

#194221

Purpose

Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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CoV2-N-T205I

Plasmid

#177954

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (T205I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (T205I) (N )

Expression

Mammalian

Mutation

T205I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pNBA001

Plasmid

#155323

Purpose

Binary vector for expression of PULSE-controlled EFR-GFP

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_(etr)8-(C120)5-PhCMVmin-EFR-GFP-T35S_RB

Use

Synthetic Biology; Eukaryotic expression

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pNBA002

Plasmid

#155324

Purpose

Binary vector for expression of PULSE-controlled GBP-mCherry

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_(etr)8-(C120)5-PhCMVmin-GBP-mCherry-T35S_RB

Use

Synthetic Biology; Eukaryotic expression

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CoV2-N-R203K

Plasmid

#177951

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (R203K) (N )

Expression

Mammalian

Mutation

R203K

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pROF417

Plasmid

#155335

Purpose

Constitutive expression LFY-VP16-2A-RLuc in plants

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

PCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos

Use

Luciferase and Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CoV2-N-G204R

Plasmid

#177953

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (G204R) (N )

Expression

Mammalian

Mutation

G204R

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Single Cassette Destination Vector (pAN2414)

Plasmid

#194220

Purpose

Backbone for constructing single casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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CoV2-N-D3L

Plasmid

#177945

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (D3L) (N )

Expression

Mammalian

Mutation

D3L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-A119S

Plasmid

#177948

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (A119S). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (A119S) (N )

Expression

Mammalian

Mutation

A119S

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-N-M234I

Plasmid

#177955

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (M234I) (N )

Expression

Mammalian

Mutation

M234I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pROF394

Plasmid

#155333

Purpose

Expression of PULSE-controlled LFY-VP16-2A-RLuc

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

(etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40

Use

Synthetic Biology; Eukaryotic expression

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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CoV2-N-D377Y

Plasmid

#177957

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D377Y). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (D377Y) (N )

Expression

Mammalian

Mutation

D377Y

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-N-P13L

Plasmid

#177946

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (P13L) (N )

Expression

Mammalian

Mutation

P13L

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge