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pCS2+ AMIC

Plasmid

#118420

Purpose

For mammalian expression of Amoeba myosin 1C

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

Amoeba myosin 1C

Expression

Mammalian

Mutation

See depositor comments below

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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A5CXB1

Plasmid

#163237

Purpose

Inducible expression of UniProt identifier A5CXB1

Depositor

Martin Engqvist

Article

Rembeza et al PLoS Comput Biol. 2021 Sep 23;17(9):e100

Insert

A5CXB1

Tags

10xHis and T7

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1-IQ-Tail

Plasmid

#187363

Purpose

Expresses rat myosin 1b isoform b IQTail domain labelled with EGFP

Depositor

Evelyne Coudrier

Article

Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

Insert

myosin 1b isoform b IQTail domain (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ rat MYO1D

Plasmid

#118419

Purpose

For mammalian expression of rat myosin 1D

Depositor

Michael Tsang

Article

Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

Insert

rat myosin 1D (Myo1d Rat)

Expression

Mammalian

Available Since

Sept. 10, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1b motor

Plasmid

#187364

Purpose

Expresses rat myosin 1b isoform b motor domain labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

Insert

myosin 1b isoform b motor domain (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-Myo1b-Tail

Plasmid

#187365

Purpose

Expresses rat myosin 1b isoform b Tail domain labelled with EGFP

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

Insert

myosin 1b isoform b Tail domain (Myo1b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-miRFP680-NES-p2A-ezrin-mRuby3-IRES-Neo

Plasmid

#222636

Purpose

Ezrin activation reporter

Depositor

Arnold Hayer

Article

Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

Insert

miRFP680-NES-p2A-ezrin-mRuby3 (EZR Human)

Use

Lentiviral

Tags

mRuby3

Promoter

EF1a

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP2A-V12-neo

Plasmid

#156178

Purpose

Lentiviral vector for expression of RAP2A-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-V12-neo

Plasmid

#156175

Purpose

Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Glycine 12 to Valine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-flag-RAP1B-N17-neo

Plasmid

#156176

Purpose

Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1B (RAP1B Bovine)

Use

Lentiviral

Tags

2xFLAG

Expression

Mammalian

Mutation

changed Serine 17 to Asparagine

Promoter

human PGK

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pMT025-P7F1-TurboV5

Plasmid

#218808

Purpose

Pax7-Foxo1-TurboV5

Depositor

Christopher Counter

Article

Zimmerman et al Nat Commun. 2026 May 28. doi: 10.1038/s4

Insert

PAX7-FOXO1-Turbo-V5 (FOXO1, PAX7 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Available Since

June 2, 2026

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh13CDS

Plasmid

#192853

Purpose

Plasmid to produce ISH probe for mMyh13 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 13 (Myh13 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh13UTR

Plasmid

#192854

Purpose

Plasmid to produce ISH probe for mMyh13 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 13 (Myh13 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.A18T

Plasmid

#99360

Purpose

Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Alanine 18 to Threonine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.E31K

Plasmid

#99361

Purpose

Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Glutamic acid 31 to Lysine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.27

Plasmid

#99362

Purpose

Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Glutamic acid 129 to Lysine

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8.110

Plasmid

#99363

Purpose

Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

Depositor

Dan Mulvihill

Article

Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

Insert

cdc8 (cdc8 Fission Yeast)

Expression

Bacterial

Mutation

Alanine 18 to Threonine and Glutamic acid 31 to L…

Promoter

T7

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh3CDS

Plasmid

#192843

Purpose

Plasmid to produce ISH probe for mMyh3 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 3 (Myh3 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh3UTR

Plasmid

#192844

Purpose

Plasmid to produce ISH probe for mMyh3 gene with UTR sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 3 (Myh3 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-mMyh6CDS

Plasmid

#192847

Purpose

Plasmid to produce ISH probe for mMyh6 gene with CDS sequence

Depositor

Masafumi Inui

Article

Tanji et al Dev Growth Differ. 2022 Dec 14. doi: 10.

Insert

myosin heavy chain 6 (Myh6 Mouse)

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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