Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 22,191 results for: his

Showing: 7201 - 7240 of 22191 results

  3. ATX3 13Q- I77K Q78K W87K

    Plasmid
    #185908
    Purpose
    Expresses ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track-I77K, Q78K,W87K (N-terminal ) fused with His-Tag and TEV cleavage sequence (C-terminal).
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track and point mutations I77K, Q78K and W87K (ATXN3 Human, Synthetic)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Point mutations in Atx3 gene I77K Q78K and W87K
    Promoter
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    185908-plasmid-map-sequence-id-370341

    Enlarge

  5. N295A (EcN), M163A (EcI) MetNIQ in pBAD

    Plasmid
    #118261
    Purpose
    N295A Mutation of MetN and M163A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (M163A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118261-plasmid-map-sequence-id-228528

    Enlarge

  6. N295A (EcN), M107A (EcI) MetNIQ in pBAD

    Plasmid
    #118259
    Purpose
    N295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (M107A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118259-plasmid-map-sequence-id-228500

    Enlarge

  7. N295A (EcN), F103A (EcI) MetNIQ in pBAD

    Plasmid
    #118258
    Purpose
    N295A Mutation of MetN and F103A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (F103A) (metI E. coli)
    MetQ (metQ E. coli)
    Use
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118258-plasmid-map-sequence-id-228498

    Enlarge

  8. SRPK1 (SRPK1A-c100)

    Plasmid
    #98246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SRPK1A (SRPK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    98246-plasmid-map-sequence-id-193287

    Enlarge

  9. UEV1

    Plasmid
    #25619
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UEV1 (UBE2V1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. SHMT2

    Plasmid
    #25479
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SHMT2 (SHMT2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    A269T
    Promoter
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25479-plasmid-map-sequence-id-168679

    Enlarge

  11. pmCellFree_KA1_SARS-COV-2_NSP16

    Plasmid
    #169389
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP16 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP16
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169389-plasmid-map-sequence-id-336165

    Enlarge

  12. MINA

    Plasmid
    #39054
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MINA (RIOX2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. PLAP

    Plasmid
    #25152
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    plap.454.738 (PLAA Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 6, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. PTPN5

    Plasmid
    #39166
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN5 (PTPN5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  15. RRAS2

    Plasmid
    #38816
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RRAS2 (Rras2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  16. SRPK2

    Plasmid
    #39047
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SRPK2 (SRPK2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Internal segment 268-518 deleted; codon-optimized
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  17. RGS16

    Plasmid
    #39140
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RGS16 (RGS16 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  18. NKTR

    Plasmid
    #25597
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NKTR (NKTR Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. TDRD3 (3PMT)

    Plasmid
    #28274
    Depositor
    Cheryl Arrowsmith
    Insert
    Tudor domain-containing protein 3 (TDRD3 Human)
    Use
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Tudor domain, residues 553-611.
    Promoter
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. INMT

    Plasmid
    #25475
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    INMT (INMT Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. rnALDH

    Plasmid
    #67920
    Purpose
    Expresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Rattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  22. pFHMSP-LIC-N

    Plasmid
    #26095
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available Since
    Sept. 15, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. SMAP1L

    Plasmid
    #25546
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SMAP1L (SMAP2 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. USP04

    Plasmid
    #25223
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    usp04.001.229 (Usp4 Mouse)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. RGS1

    Plasmid
    #39143
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RGS1 (RGS1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  26. NRDP1

    Plasmid
    #25620
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NRDP1 (RNF41 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains USP8 interaction Domain
    Promoter
    Available Since
    Aug. 31, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. SLK

    Plasmid
    #38963
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SLK (SLK Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. Pf-GMPS

    Plasmid
    #36899
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pf-GMPS (PF10_0123 Plasmodium falciparum)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. NUDT7 (NUDT7A-c002)

    Plasmid
    #98235
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NUDT7A (NUDT7 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98235-plasmid-map-sequence-id-193415

    Enlarge

  30. PDLIM5

    Plasmid
    #38811
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PDLIM5 (Pdlim5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. RAMP2

    Plasmid
    #39058
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RAMP2 (RAMP2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. MLYCD

    Plasmid
    #39081
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MLYCD (MLYCD Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Surface mutant for crystallisation E19A, K20A, E2…
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  33. PBC002-A04

    Plasmid
    #58761
    Purpose
    bacterial expression of PDB 4PWY
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4PWY (PEX6 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    Aug. 5, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. UXS1

    Plasmid
    #39162
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    UXS1 (UXS1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  35. pmCellFree_KA1_SARS-COV-2_NSP10

    Plasmid
    #169384
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus NSP10 with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _NSP10
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169384-plasmid-map-sequence-id-336496

    Enlarge

  36. PHIP

    Plasmid
    #39049
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PHIP (PHIP Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. UBQLN1

    Plasmid
    #25300
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh29.034.112 (UBQLN1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. pmCellFree_KA1_SARS-COV-2_ORF3b

    Plasmid
    #169391
    Purpose
    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF3b with N-term eGFP
    Depositor
    Kirill Alexandrov
    Insert
    SARS-COV-2 _ORF3b
    Use
    Cell-free expression vector
    Tags
    His and eGFP
    Expression
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    T7- CMV/CAG
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    169391-plasmid-map-sequence-id-336874

    Enlarge

  39. UBA05 (3GUC)

    Plasmid
    #25212
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    uba05.057.329 (UBA5 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. PP-HSP70 (3L4I)

    Plasmid
    #25291
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    cgd2_20:G15-E396
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains amino acids G15-E396
    Promoter
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 7201 - 7240 of 22191 results