We narrowed to 27,982 results for: STI

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Polymerase expression - pCMV-ePhi29 (LM2802)

Plasmid

#208967

Purpose

Unfused ePhi29 DNA polymerase (-exo), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

ePhi29-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBullet-tgn-c

Plasmid

#53072

Purpose

destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

Plasmid

#208969

Purpose

Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

EcKlenow-BPNLS-CC(N5)

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBullet-end-c

Plasmid

#53074

Purpose

destination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-GFP-ATL1-G20TM

Plasmid

#109019

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL1-deltaTM (ATL1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

truncated form without C-terminal ER transmembran…

Promoter

EIF-1a promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_BRD111

Plasmid

#78166

Purpose

Expresses Cas9 with the pLX311 backbone

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

Cas9

Use

Lentiviral

Tags

3xFLAG and NLS

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
Lenti-EFS-hTSC1-T2A-Bsd

Plasmid

#193199

Purpose

Lentiviral vector expressing human TSC1 cDNA (driven by EFS promoter) and a blasticidin resistance marker

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

TSC1 (TSC1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRV-RB

Plasmid

#106374

Purpose

creating stable line with RFP and BSD resistance

Depositor

Tong-Chuan He

Article

Liao et al Gene Ther. 2017 Jun;24(6):333-341. doi:

Insert

mRFP and blasticidin resistance gene

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs_SFXN1

Plasmid

#110635

Purpose

Retroviral expression of SFXN1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN1 (SFXN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBullet-pm-c

Plasmid

#53078

Purpose

destination vector with PIP2a-ECFP (plasma membrane marker) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-cg-c

Plasmid

#53070

Purpose

destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-112–858_Flag2-Blast

Plasmid

#175039

Purpose

Mammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein from amino acid positi…

Promoter

CAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-∆ARD_Flag2-Blast

Plasmid

#175041

Purpose

Mammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein with deletion of the a…

Promoter

CAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS

Plasmid

#59388

Purpose

Destination vector with RBPMS for stable cell line generation

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBullet-vac-c

Plasmid

#53076

Purpose

destination vector with vacuole marker (gamma-TIP) - ECFP for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-er-c

Plasmid

#53068

Purpose

destination vector with WAK2 (29 aa)-ECFP- ER marker (HDEL) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pBullet-cyt-c

Plasmid

#53066

Purpose

destination vector with cytosol marker (ECFP) for tagged fluorescent protein (C-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Tags

ECFP and EYFP

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL1-5

Plasmid

#109007

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL1-sgRNA #5 (ATL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBullet-cg-n

Plasmid

#53069

Purpose

destination vector with cis-golgi marker (alpha-man I 49AA) -ECFP for tagged fluorescent protein (N-ter-EYFP) of plant gene

Depositor

Joshua Heazlewood

Article

Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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BMR1_02g02691-bio-His

Plasmid

#108042

Purpose

Expresses enzymatically monobiotinylated full-length BMR1_02g02691 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

Insert

BMR1_02g02691

Tags

ratCD4d3+4,biotinylation sequence, 6xHis (C termi…

Expression

Mammalian

Mutation

all potential N-linked glycosylation sites (N-X-S…

Promoter

CMV

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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