We narrowed to 165,343 results for: addgene

1. pcDNA3.1/TO-myc-BirA*-NES

   Plasmid

   #112714

   Purpose

   For tetracycline-inducible expression of myc-BirA*-NES in mammalian cells. For BioID studies.

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   HIV Rev nuclear export signal (NES)
   

   Expression

   Mammalian

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiMutB3GALT5-blast

   Plasmid

   #208381

   Purpose

   lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nucleotide change in PAM targeting sequence nt 51…

   Promoter

   EF-1 alpha core promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lentiMutB3GALT5catdead-blast

   Plasmid

   #220869

   Purpose

   lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   encodes D156A and D243A mutations; also, nucleoti…

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TAL2430

   Plasmid

   #36829

   Depositor

   Keith Joung

   Article

   Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

   Insert

   HDAC2-TALEN_Left (Hdac2 Human)
   

   Use

   Mammalian, t7 for in vitro mrna synthesis

   Tags

   3X Flag and WT FOKI

   Promoter

   CMV

   Available Since

   June 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. MAC_C_SCO1

   Plasmid

   #111675

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   SCO1 (SCO1 Human)
   

   Expression

   Mammalian

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCW-GFP-CtIP-T847E

   Plasmid

   #71111

   Purpose

   mammalian expession of GFP-CtlP T847E mutation

   Depositor

   Daniel Durocher

   Article

   Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

   Insert

   CtlP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   T847E

   Promoter

   TRE promoter, Tet ON

   Available Since

   Dec. 9, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. CAG-Otx2-IRES-GFPd2

   Plasmid

   #73996

   Purpose

   Plasmid expressing Otx2(isoform b)-IRES-GFPd2

   Depositor

   Connie Cepko

   Article

   Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

   Insert

   Otx2(isoform b)-Ires-GFP (Otx2 Mouse)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MSCV PIG Myb

   Plasmid

   #66988

   Purpose

   Retroviral expression plasmid for mouse Myb expression; containing bicistronic IRES-GFP

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Myb (Myb Mouse)
   

   Use

   Retroviral

   Promoter

   LTR

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG306-GPD-VMA1 chr I

   Plasmid

   #41896

   Purpose

   Yeast expression of VMA1.

   Depositor

   Dan Gottschling

   Article

   Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

   Insert

   VMA1 (VMA1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   Contains silent mutation at 342A-G and L87S mutat…

   Promoter

   GPD

   Available Since

   April 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. THI VP64 pALD4 T-

    Plasmid

    #161481

    Purpose

    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    

    Expression

    Yeast

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEm-CoilE-Actin

    Plasmid

    #181993

    Purpose

    CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    mEm-CoilE-Actin
    

    Tags

    mEmerald

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLKO-puro-sh-mFAK C

    Plasmid

    #37015

    Depositor

    Bob Weinberg

    Article

    Shibue et al Proc Natl Acad Sci U S A. 2009 Jun 23;10

    Insert

    sh-mFAK C (Ptk2 Mouse)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 31, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pLenti-eGFP-dSaCas9-blast

    Plasmid

    #191653

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    eGFP-T2A-dSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RP1B-PP1alpha

    Plasmid

    #51768

    Purpose

    Expresses PP1 alpha residues 7-330

    Depositor

    Wolfgang Peti

    Article

    Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

    Insert

    pp1 alpha
    

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 alpha residues 7-330

    Promoter

    T7-lac

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

15. OLIG2_pCSdest

    Plasmid

    #53858

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    OLIG2 (OLIG2 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pLenti_TMv2_Zeo

    Plasmid

    #131762

    Purpose

    frameshift reporter for barcode retrieval (with Zeo selection)

    Depositor

    Paul Blainey

    Article

    Feldman et al BMC Biol. 2020 Nov 24;18(1):177. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNoTag-DGCR8

    Plasmid

    #164694

    Purpose

    Express DGCR8 without tag in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    DGCR8 (DGCR8 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBABE Hygro ST6GalNaC5

    Plasmid

    #26981

    Depositor

    Joan Massague

    Article

    Bos et al Nature. 2009 Jun 18. 459(7249):1005-9.

    Insert

    ST6GalNaC5 (ST6GALNAC5 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-LAP1_2-mCherry

    Plasmid

    #159526

    Purpose

    Expresses mCherry from LAP1_2 promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

    Insert

    mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    LAP1_2

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PRDM15_pCSdest

    Plasmid

    #53907

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    PRDM15 (PRDM15 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits