We narrowed to 4,056 results for: lenti crispr plasmid

1. mPLD3-sgRNA-Cas9-mcherry

   Plasmid

   #199700

   Purpose

   encodes sgRNA for mouse PLD3 KO, (target 217-223aa) plus Cas9-P2A-mCherry

   Depositor

   David Nemazee

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   P2A-RFP

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

   Plasmid

   #50946

   Purpose

   An empty gRNA expression vector

   Depositor

   Kosuke Yusa

   Article

   Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

   Insert

   None
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMV_AA099

   Plasmid

   #245321

   Purpose

   Cas9 CRISPRko all-in-one positive control; targets CD59

   Depositor

   John Doench, David Root

   Insert

   Cas9, sgRNA CD59 (CD59 Synthetic)
   

   Use

   Lentiviral

   Mutation

   None

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. SGEP-sh-Hs-PHGDH-67

   Plasmid

   #188671

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. SGEP-sh-Hs-PHGDH-1956

   Plasmid

   #188670

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. SGEP-sh-Hs-PHGDH-1883

   Plasmid

   #188669

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SGEP-sh-Hs-PHGDH-1968

   Plasmid

   #188673

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SGEP-sh-Hs-PHGDH-1957

   Plasmid

   #188674

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SGEP-sh-Hs-PHGDH-1967

   Plasmid

   #188672

   Purpose

   shRNA

   Depositor

   Lewis Cantley

   Insert

   shPHGDH (PHGDH Human)
   

   Use

   CRISPR and Lentiviral

   Mutation

   WT

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SGL40C.EFS.dTomato

    Plasmid

    #89395

    Purpose

    Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAIO-Ef1a-PE2-GFP:KCNQ2-C201R_pp

    Plasmid

    #185061

    Purpose

    Ef1a driven PE2 with pegRNA for editing C201R mutation (including silent PAM mutation) in KCNQ2. See Addgene plasmid #184445

    Depositor

    Bobby Koeleman

    Insert

    U6:pegRNA:scaffold:PBS+RT template
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. LRG2.1-CyOFP1

    Plasmid

    #124771

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and CyOFP1

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. LRG2.1-mOrange

    Plasmid

    #124772

    Purpose

    3rd generation lentiviral plasmid to co-express a guide RNA and mOrange

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. SiC-V1

    Plasmid

    #133041

    Purpose

    SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

    Depositor

    Sriram Neelamegham

    Article

    Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

    Insert

    SpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    dTomato

    Available Since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid

    #90085

    Purpose

    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be remove for cloning of desired sgRNA

    Depositor

    Raffaella Sordella

    Article

    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Destabilized Domain and Flag

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid

    #90086

    Purpose

    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA

    Depositor

    Raffaella Sordella

    Article

    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Destabilized Domain and Flag

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. HK1 gRNA (BRDN0001145157)

    Plasmid

    #77643

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human HK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    HK1 (HK1, HOOK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. HK1 gRNA (BRDN0001147564)

    Plasmid

    #77644

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human HK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    HK1 (HK1, HOOK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HK1 gRNA (BRDN0001146994)

    Plasmid

    #77645

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human HK1

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    HK1 (HK1, HOOK1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

    Plasmid

    #117136

    Purpose

    Empty vector encoding BFP-BSD

    Depositor

    Kosuke Yusa

    Insert

    BSD2A
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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