We narrowed to 10,318 results for: plasmids 101

1. pAPInt-Dendra2-G418

   Plasmid

   #236496

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   Dendra2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAPInt-mScarlet-G418

   Plasmid

   #236493

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with mScarlet and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   mScarlet
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPInt-GFP-G418

   Plasmid

   #236478

   Purpose

   Plasmid for deleting or C-terminally tagging endogenous genes with GFP and selection on G418.

   Depositor

   Daniel Lew

   Article

   Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Codon optimized for A. pullulans

   Promoter

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-empty

   Plasmid

   #239099

   Purpose

   pGL3 luciferase vector with no promoter.

   Depositor

   David Cobrinik

   Article

   Shayler et al eLife 13:RP101918

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXs-hOCT3/4

   Plasmid

   #17217

   Purpose

   Retroviral expression of human OCT3/4

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

   Insert

   OCT3/4 (POU5F1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGl3-Camk2g

   Plasmid

   #186821

   Purpose

   Fluorescent reporter for Camk2g expression

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2g promoter (Camk2g Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Camk2g promoter

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-mTagBFP2-P2A-EGFP

   Plasmid

   #184943

   Purpose

   Expresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   mTagBFP2-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CAG-mBlueberry2-P2A-EGFP

   Plasmid

   #184937

   Purpose

   Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   mBlueberry2-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-Electra2-P2A-EGFP

   Plasmid

   #184936

   Purpose

   Expresses blue fluorescent protein Electra2 and EGFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   Electra2-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

    Plasmid

    #39454

    Depositor

    Hongjun Song

    Article

    Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

    Insert

    HA-hTet1CD (TET1 Human)
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    aa1418–2136

    Promoter

    EF1a

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-CAG-EBFP2-P2A-EGFP

    Plasmid

    #184938

    Purpose

    Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    EBFP2-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-CAG-Electra1-P2A-EGFP

    Plasmid

    #184935

    Purpose

    Expresses blue fluorescent protein Electra1 and EGFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    Electra1-P2A-EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Krox20 promoter pGL3-TATA

    Plasmid

    #21260

    Depositor

    Jerry Crabtree

    Article

    Kao et al Science. 2009 Jan 30. 323(5914):651-4.

    Insert

    Krox20 promoter (Egr2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. PGL3-Ogt (-178 to +233 bp)

    Plasmid

    #79266

    Purpose

    To identify the Cis and Trans acting elements that control Ogt gene transcription.

    Depositor

    Steven Jones

    Article

    Muthusamy et al J Biol Chem. 2015 Dec 25;290(52):31013-2

    Insert

    Ogt promoter element (Ogt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

15. AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid

    #60231

    Purpose

    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Cre recombinase
    EGFP
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Tags

    Cre-HA-P2A and EGFP-KASH

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hSyn

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

    Plasmid

    #60226

    Purpose

    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

    Depositor

    Feng Zhang

    Article

    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

    Inserts

    sgRNA
    Renilla luciferase
    Cre recombinase
    

    Use

    AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

    Tags

    Cre-HA and Rluc-P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available Since

    Oct. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-ABE8.20m-SpCas9-NRTH-P2A-EGFP (RMD63)

    Plasmid

    #197500

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE(8.20) A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8.20m-nSpCas9-NRTH-BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8.20 mutations in TadA and nSpCas9-NRTH(D10A/I…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-TadCBEd-SpG-P2A-EGFP (BKS777/AHK571)

    Plasmid

    #223124

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd-SpG with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadCBEd-SpG-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpG=D10A/D1135L/S1136W/G1218…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLY57_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2-TRAC-RHA(3166mut)

    Plasmid

    #192188

    Purpose

    CD22 CAR AAV vector PRODH2 KI (pLY057)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    CD22 CAR AAV vector PRODH2 KI (pLY057) (CD22 Synthetic)
    

    Use

    AAV; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLY59_TRAC-LHA-pAAV-EFS-CD22BBz-PRODH2(stop)-TRAC-RHA(3166mut)

    Plasmid

    #192189

    Purpose

    CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059)

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    CD22 CAR AAV vector PRODH2(StopCtrl) (pLY059) (CD22 Synthetic)
    

    Use

    AAV; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge