We narrowed to 9,265 results for: yeast expression

1. pJT112_GalL_nCDA1-xBE3

   Plasmid

   #141318

   Purpose

   Expressing base editor nCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-xBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL59-Nat

   Plasmid

   #140465

   Purpose

   CRISPR/Cas9 engineering with nourseothricin selection

   Depositor

   Gianni Liti

   Article

   Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. BB3aN_14*

   Plasmid

   #98548

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PhyB-mCherry-MYO1

   Plasmid

   #51570

   Purpose

   Constitutively express myosin ring localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   MYO1 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT45_GalL_nCDA1-BE3

   Plasmid

   #145038

   Purpose

   Expresses nCDA1-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. BB3aK_14*

   Plasmid

   #98529

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT79_GalL_nCDA1Δ192-BE3

   Plasmid

   #145045

   Purpose

   Expresses nCDA1Δ192-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ192-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   pmCDA1(1-192AA); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. BB3rN_AH

   Plasmid

   #98556

   Purpose

   empty BB3 for insertion and overexpression of 7 genes in P. pastoris

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCY5.1kh

   Plasmid

   #160297

   Purpose

   Yeast CRISPR plasmid targeting the kanMX and hphMX cassettes

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
   URA3MX selection cassette
   sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYI277

    Plasmid

    #228285

    Purpose

    Constitutive EGFP expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    KpGAPDH promoter

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCY5.1nh

    Plasmid

    #160299

    Purpose

    Yeast CRISPR plasmid targeting the natMX and hphMX cassettes

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCY5.1kn

    Plasmid

    #160298

    Purpose

    Yeast CRISPR plasmid targeting the kanMX and natMX cassettes

    Depositor

    Amir Aharoni

    Article

    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

    Inserts

    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    

    Use

    CRISPR

    Tags

    8xHis-tag and SV40 NLS

    Expression

    Mutation

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCas9-EcRT-Z3-Kan

    Plasmid

    #232096

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    Z3 promoter and Z3EV transcription factor
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Z3

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pACT2-μ4

    Plasmid

    #198176

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-4 μ4
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGADT7-AP-2 σ2

    Plasmid

    #198345

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

    Insert

    AP-2 σ2
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    R42G substitution, and silent substitutions in co…

    Promoter

    ADH1

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pACT2-μ1A

    Plasmid

    #198168

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1A fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-1 μ1A
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACT2-μ2

    Plasmid

    #198173

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-2 μ2
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDB4961

    Plasmid

    #174015

    Purpose

    pHIS3H plasmid expressing 13Myc-Vps15 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Vps15 (ppk19 Fission Yeast)
    

    Use

    Tags

    13Myc, Vps15

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. EC2_2_dCas9_VP64_sgRNA

    Plasmid

    #163707

    Purpose

    Yeast low copy plasmid with dCas9, sgRNA expression cassette and VP64 activation domain

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Inserts

    dCas9
    VP64+SV40 NLS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDB4946

    Plasmid

    #174018

    Purpose

    pDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoter

    Depositor

    Li-Lin Du

    Article

    Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

    Insert

    Atg14 (atg14 Fission Yeast)
    

    Use

    Tags

    Pil1, mCherry, Atg14

    Expression

    Yeast

    Mutation

    Promoter

    41nmt1

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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