We narrowed to 5,111 results for: codon optimized

1. pJJGL002

   Plasmid

   #180606

   Purpose

   Plasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasX

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Insert

   PlmCasX
   

   Use

   CRISPR

   Tags

   10xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-c/o NL4-3 Env delta CT

   Plasmid

   #139285

   Purpose

   HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   codon optimized HIV-1 Env delta cytoplasmic tail
   

   Expression

   Mammalian

   Mutation

   deletion of cytoplasmic tail after residue 710

   Promoter

   CMV IE

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nsp7-EGFP

   Plasmid

   #165111

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp7 (ORF1ab codon optimized SARS-COV-2)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-FRT-TO-FH-Nsp16

   Plasmid

   #157724

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp16 (ORF1ab SARS-CoV-2)
   

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCC_01 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9-NLS-2A-Puro-WPRE

   Plasmid

   #139086

   Purpose

   Expresses human codon-optimized SpCas9 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   SpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ATG-1929

   Plasmid

   #108712

   Purpose

   CBR2opt in pF4Ag expression vector with T7 and CMV promoter

   Depositor

   Keith Wood

   Article

   Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

   Insert

   CBR2opt
   

   Expression

   Bacterial and Mammalian

   Mutation

   Promega used directed evolution to create CBR (Cl…

   Promoter

   T7, CMV

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT-TO-FH-Nsp14

   Plasmid

   #157718

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp14 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp14 (ORF1ab SARS-CoV-2)
   

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Available Since

   Aug. 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pBT3000

   Plasmid

   #122536

   Purpose

   Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2

   Depositor

   Gary Silverman

   Article

   Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

   Insert

   Pnhx-2_CemOrange2::tram-1 (tram-1 Synthetic, Nematode)
   

   Expression

   Bacterial

   Promoter

   Pnhx-2

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-BG505.SOSIP-QES.i03.c01-8his

   Plasmid

   #111846

   Purpose

   Mammalian expression plasmid for soluble BG505 SOSIP.664; QES mutant with enhanced binding to PG16

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Tags

   8his purification tag and CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid

    #113960

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-563

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TO-Nsp10-HF

    Plasmid

    #157708

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp10-HF (ORF1ab SARS-CoV-2)
    

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pAAV2-CAGIG-H2B-V5-mScarlet

    Plasmid

    #191093

    Purpose

    To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

    Depositor

    Murray Blackmore, Pantelis Tsoulfas

    Article

    Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

    Insert

    H2B and mScarlet
    

    Use

    AAV

    Tags

    H2B-V5-mScarlet

    Expression

    Mammalian

    Mutation

    The fusion protein was optimized to the Human cod…

    Promoter

    CMV and CAG

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-HLA-cmyc-optiT1R3 a21-852

    Plasmid

    #113948

    Purpose

    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Tags

    Signal/leader sequence from HLA class I histocomp…

    Expression

    Mammalian

    Mutation

    residues 21-852

    Promoter

    CMV

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCEP4-BG505.SOSIP-gp160-QES.i05.c06

    Plasmid

    #123276

    Purpose

    Mammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations); QES mutant for enhanced presentation of quaternary epitopes

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BG505) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; SOSIP mutations; …

    Promoter

    CMV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW-Cdt1ΔCy-mCherry-Puro

    Plasmid

    #193764

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1ΔCy (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1ΔCy-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Mutation

    Cy motif (a.a. 68-70) mutated to alanine, a.a. 11…

    Promoter

    TRE

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-4F2-mCherry

    Plasmid

    #73421

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P4F2 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBT3011

    Plasmid

    #122539

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal localized gene (cup-5) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::cup-5 (cup-5 Synthetic, Nematode)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. eSpCas9-plus

    Plasmid

    #126767

    Purpose

    Expression plasmid for human codon-optimized increased fidelity eSpCas9-plus (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBT3003

    Plasmid

    #122537

    Purpose

    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (pisy-1) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_CemOrange2::pisy-1 (pisy-1 Synthetic, Nematode)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9-NRCH-APOBEC-nCas9-Ung

    Plasmid

    #198552

    Purpose

    Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NRCH-APOBEC-nCas9-Ung
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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