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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,422 results for: nls

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Showing: 3621 - 3640 of 6422 results

  4. pKG194

    Plasmid
    #69757
    Purpose
    Mammalian Expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and eGFP
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. P2814

    Plasmid
    #248829
    Purpose
    All repressors for the WTC848 system.
    Depositor
    Joerg Stelling
    Insert
    LacI(6m), LacI(5m)
    Tags
    SV40NLS on LacI(6m)
    Expression
    Yeast
    Mutation
    LacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…
    Promoter
    pRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248829-plasmid-map-sequence-id-497158

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  10. P2880

    Plasmid
    #248830
    Purpose
    All repressors for the WTC848 system.
    Depositor
    Joerg Stelling
    Insert
    LacI(6m), LacI(5m)
    Tags
    SV40NLS on LacI(6m)
    Expression
    Yeast
    Mutation
    LacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…
    Promoter
    pRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248830-plasmid-map-sequence-id-497160

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  11. P2881

    Plasmid
    #248831
    Purpose
    All repressors for the WTC848 system.
    Depositor
    Joerg Stelling
    Insert
    LacI(6m), LacI(5m)
    Tags
    SV40NLS on LacI(6m)
    Expression
    Yeast
    Mutation
    LacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…
    Promoter
    pRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248831-plasmid-map-sequence-id-497164

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  12. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

    Plasmid
    #246980
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    FLP Recombinase
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246980-plasmid-map-sequence-id-491526

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  13. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195623
    Purpose
    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195623-plasmid-map-sequence-id-385117

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  14. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195624
    Purpose
    pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Use
    AAV
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195624-plasmid-map-sequence-id-385116

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  15. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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Showing: 3621 - 3640 of 6422 results