Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 18,759 results for: nls

Clear keywords
Showing: 3621 - 3640 of 18759 results

  2. pDR111_mannose_Bgal NLS

    Plasmid
    #188398
    Purpose
    Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) and nuclear localization signal (SV40) that is fused to beta-galactosidase.
    Depositor
    Christopher Contag
    Publication
    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:
    Insert
    lacZ
    Tags
    PhoD secretion peptide and SV40 NLS
    Expression
    Bacterial
    Promoter
    D-mannose
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188398-plasmid-map-sequence-id-370473

    Enlarge

  3. pcDNA3-P4:gs:nls:FKBP

    Plasmid
    #135984
    Purpose
    Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    P4 coiled-coil fused to the FKBP domain
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135984-plasmid-map-sequence-id-270610

    Enlarge

  4. pcDNA3-His:FRB:nls:VPR

    Plasmid
    #135987
    Purpose
    Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells
    Depositor
    Roman Jerala
    Publication
    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FRB domain fused to the VPR activation domain
    Tags
    His
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    135987-plasmid-map-sequence-id-268598

    Enlarge

  5. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

    Enlarge

  6. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

    Enlarge

  7. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid
    #204619
    Purpose
    Expression vector of Cascade and Cas3. Puromycin selectable.
    Depositor
    Akitsu Hotta
    Publication
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    Use
    CRISPR
    Tags
    Each protein has C- and N-terminal SV40 NLS
    Promoter
    EF1a
    Available since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    204619-plasmid-map-sequence-id-404756

    Enlarge

  9. TV_EMP1-ires-mNeon-nls-p2a-icasp9-wpre_pgk-puro

    Plasmid
    #251175
    Purpose
    Targeting vector for C-terminal knockin at EMP1 locus to enable reporting of expression by mNeonGreen and depletion of positive cells with iCasp9
    Depositor
    Hugo Snippert
    Insert
    ires-mNeon-nls-p2a-icasp9-wpre_pgk-puro flanked by homology arms for C-terminal targeting of human EMP1
    Use
    CRISPR
    Tags
    ires-mNeon-nls-p2a-icasp9
    Available since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251175-plasmid-map-sequence-id-500692

    Enlarge

  12. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

    Enlarge

  15. A3Ai E72A-Cas9n-UGI-NLS

    Plasmid
    #109430
    Purpose
    Expresses catalytically inactive human APOBEC3A with an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Reuben Harris
    Publication
    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A E72A Catalytic Mutant (APOBEC3A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    109430-plasmid-map-sequence-id-212945

    Enlarge

  16. TV_ANXA1-p2a-mScarlet-nls-p2a-icasp9_pgk-blast

    Plasmid
    #251171
    Purpose
    Targeting vector for C-terminal knockin at ANXA1 locus to enable reporting of expression by mScarlet and depletion of positive cells with iCasp9.
    Depositor
    Hugo Snippert
    Insert
    p2a-mScarlet-nls-icasp9_pgk-blast flanked by homology arms for C-terminal targeting of human ANXA1 (ANXA1 Human)
    Use
    CRISPR
    Tags
    p2a-mScarlet-nls-p2a-icasp9
    Available since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251171-plasmid-map-sequence-id-500693

    Enlarge

  18. pSL103-SpCas9-FLAG-3XNLS

    Plasmid
    #182032
    Purpose
    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP
    Depositor
    Steven Lin
    Publication
    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do
    Insert
    Streptococcus pyogenes cas9 (cas9 Synthetic)
    Use
    CRISPR
    Tags
    3XNLS, 12X His tag, FLAG, and MBP
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182032-plasmid-map-sequence-id-360101

    Enlarge

Showing: 3621 - 3640 of 18759 results