We narrowed to 6,501 results for: nls
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Plasmid#85755PurposeExpresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCsy4-T2A-FokI-SadCas9
UseCRISPRTags3xHA tag, Csy4, and FokI fusion via 36 amino acid…ExpressionMammalianMutationD10A, N580APromoterCAGAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTL53_pyCAG-s36GFPmNG-IRES-mCherry3NLS-polyA
Plasmid#223545PurposeMinimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by IRES, strong constitutive promoter CAG, no insulation or mammalian selection geneDepositorInserts36GFP-mNeonGreen, mCherry-3NLS
ExpressionMammalianPromoterCAGAvailable sinceJan. 21, 2026AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-MCP-3XBFP-2xNLS
Plasmid#236371PurposeOverexpression of MCP-3XBFP-2xNLS in human cellsDepositorInsertMCP-3XBFP-2xnls
UseLentiviralAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc LbaCas12a
Plasmid#182125PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc LbaCas12a
Tags6xHisExpressionBacterialPromoterT7Available sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc enAspCas12a
Plasmid#182127PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc enAspCas12a
Tags6xHisExpressionBacterialPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc AspCas12a
Plasmid#182122PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc AspCas12a
Tags6xHisExpressionBacterialPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1026-Tier1-PhCMV-3xNLS-iRFP670
Plasmid#169532PurposeTier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPCMV-driven nucleus-localized far-red fluorescent protein iRFP670
ExpressionMammalianPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-Nvec-codonopti-Cas9-2xNLS
Plasmid#141108PurposePlasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensisDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNematostella codon optimized Cas9
UseUnspecifiedMutationAll amino acids coded for by codons optimized for…PromoterT7Available sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS
Plasmid#106353PurposeCRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locusDepositorInsertD4Z4 gRNA-2
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NRCH-YE1-BE4max
Plasmid#198549PurposeExpresses human codon-optimized SpCas9-NRCH-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NRCH-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NRCH-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
VRQR-HF1 variant
Plasmid#138563PurposeExpresses human codon-optimized SpCas9-VRQR-HF1 and blasticidin resistance: EFS promoter-VRQR-HF1-NLS-FLAG-P2A-BSDDepositorInsertVRQR-HF1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926A, D1135V, G1218R, R1335…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
QQR1 variant
Plasmid#138564PurposeExpresses human codon-optimized SpCas9-QQR1 and blasticidin resistance: EFS promoter-QQR1-NLS-FLAG-P2A-BSDDepositorInsertQQR1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationG1218R, N1286Q, I1331F, D1332K, R1333Q, R1335Q, T…PromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV_CROPseq_v2-2xMycNLS-mScarlet
Plasmid#254798PurposePlasmid backbone for guide cloningDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable sinceAug. 31, 2026AvailabilityAcademic Institutions and Nonprofits only -
CMV_dCas9-AsLOV2x3-NLSx3
Plasmid#231567PurposeExpresses components of the LOOMINA optogenetic transcriptional control system.DepositorInsertdCas9-AsLOV2x3
UseCRISPRExpressionMammalianAvailable sinceFeb. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LbCas12a-8xNLS
Plasmid#209020PurposeLentiviral vector expressing human codon-optimized LbCas12a with 8xNLSDepositorInsertLbCas12a-8xNLS-Myc-NeoR
UseCRISPR and LentiviralTagsMYCExpressionMammalianPromoterEF-1aAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
LFNG:GFPnls-T2a-ER-Cre-ER
Plasmid#200107DepositorInsertLFNG:GFPnls-T2A-ER-Cre-ER
UseUnspecifiedAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKOet-PCNA-19-SV40NLS-4
Plasmid#57933PurposeLocalization: Nucleus, Excitation: 548, Emission: 561DepositorAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBG-hBEN-deltaNLS3
Plasmid#22161DepositorInserthBEN (GTF2IRD1 Human)
TagsGST and HisExpressionMammalianMutationDeletion of amino acids 883-889Available sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 MEKK2 KD
Plasmid#12183DepositorAvailable sinceSept. 22, 2006AvailabilityAcademic Institutions and Nonprofits only -
SpRY
Plasmid#198544PurposeExpresses human codon-optimized SpRY and blasticidin resistance: EFS promoter-SpRY-NLS-FLAG-P2A-BSDDepositorInsertSpRY
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only