We narrowed to 4,377 results for: p2a

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pJM524 EF1α 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161553

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM525 EF1α 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA in TUPV2

Plasmid

#161554

Purpose

Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-DsDed-intC-ZF1-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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LRG-neo-sgTAF12(mouse)#4.1

Plasmid

#105588

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting TAF12 HFD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-neo-sgMYB(human)#4.9

Plasmid

#105591

Purpose

lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human MYB DBD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-neo-sgMYB(human)#5.2

Plasmid

#105592

Purpose

lentivirally express gRNA targeting human MYB DBD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human MYB DBD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSN34

Plasmid

#100119

Purpose

expresses a chimeric OCT4-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

vexGFP-P2A-OCT4 fused to 2 VP16 domains (413-460) separated by linkers (POU5F1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pPKm-241

Plasmid

#104855

Purpose

pSIN-cytosFD-P2A-cytosFNR, expressing Synechococcus sp Fd and FNR, under EF-1alpha promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

cytosFd-cytosFNR

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-wrd

Plasmid

#195070

Purpose

Expresses FLAG-tagged Drosophila wrd protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

well-rounded (wrd Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMIG-Aalpha E64G

Plasmid

#10885

Depositor

William Hahn

Article

Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

Insert

PP2A Aalpha (PPP2R1A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

E64G

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pMIG-Aalpha WT

Plasmid

#10884

Depositor

William Hahn

Article

Chen et al Cancer Res. 2005 Sep 15. 65(18):8183-92.

Insert

PP2A Aalpha (PPP2R1A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pUb FLAG-tws

Plasmid

#195068

Purpose

Expresses FLAG-tagged Drosophila tws protein

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

twins (tws Fly)

Tags

3xFLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCas9-EGFP

Plasmid

#63592

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Phil Sharp, Feng Zhang

Article

Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

Inserts

Cas9
EGFP

Use

CRISPR and Lentiviral

Tags

FLAG and P2A

Expression

Mammalian

Promoter

EFS-NS

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-ATF5-PROX1-HNF1A

Plasmid

#149725

Purpose

expressing three liver promoting transcription factors (ATF5, PROX1, HNF1A)

Depositor

Baisong Lu

Article

Lu et al Biomaterials. 2021 Feb;269:120668. doi:

Insert

ATF5-PROX1-HNF1A (ATF5 Human)

Use

Lentiviral

Tags

P2A, T2A

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-Fluc-PGK-Rluc-mCherry

Plasmid

#191863

Purpose

Heat inducible Fluc reporter with constitutive Rluc

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

Firefly luciferase Fluc
Renilla luciferase Rluc

Use

Lentiviral

Tags

P2A-mCherry

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pNP3386 [For use in genome insertion and off-target assessment]

Plasmid

#247258

Purpose

Example plasmid for bridge recombinase mediated insertion and off-target assessment with puromycin selection — must clone in UMI

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

ISCro4 Recognition Site-UMI-Ef1a-PuroR-P2A-mCherry (Plasmid for Insertion Site Mapping [Example, Clone in Sequence + UMI])

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pEF_PE2

Plasmid

#199267

Purpose

Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

PE2-P2A-mp53DD

Expression

Mammalian

Mutation

Δ40-903

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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PB-PE

Plasmid

#162796

Purpose

All-in-one prime editor piggyBac transposon

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_ H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pENN236_CRISPRcharm_Kv2

Plasmid

#220839

Purpose

CRISPRcharm Kv2 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv2

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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CROPseq-Guide-Puro-mCherry2

Plasmid

#219679

Purpose

CROPseq vector based on #86708 with an additional mCherry2 fluorescent gene

Depositor

Christoph Bock

Article

Boccuni et al Sci Signal. 2022 Dec 13;15(764):eabq5389

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EF1a-Puro-P2A-mCherry2-WPRE-hU6-gRNA

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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