We narrowed to 4,225 results for: pCMV

1. PEA1-GFP

   Plasmid

   #171993

   Purpose

   Delivers all prime editing nickase components in a single, GFP selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-GFP, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CGBE1 (pRZ3885)

   Plasmid

   #140252

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. 2xmyc-RAB10

   Plasmid

   #164631

   Purpose

   Expresses RAB10 in mammalian cells with a 2xmyc tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   Ras-related protein Rab-10 (RAB10 Human)
   

   Tags

   2xmyc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5-Flag-Med25

   Plasmid

   #64771

   Purpose

   Mammalian protein expression of flag-tagged Med25

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

   Insert

   Med25 (MED25 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

5. Frt-V5-hspB1

   Plasmid

   #63102

   Purpose

   Expression of HSPB1 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb1 (HSPB1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. 12xMoonTag-12xSunTag-kif18b-24xPP7

   Plasmid

   #128606

   Purpose

   Expresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Inserts

   12xMoonTag-12xSunTag
   kinesin family member 18b (KIF18B Human)
   

   Expression

   Mammalian

   Promoter

   Pcmv

   Available Since

   Aug. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV_Npu-saBE3.9max C-terminal

   Plasmid

   #137180

   Purpose

   Expresses the C-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-saBE3.9max_C-terminal
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV_Npu-saBE3.9max N-terminal

   Plasmid

   #137179

   Purpose

   Expresses the N-terminal split-intein fragment of S. aureus BE3.9max from the CMV promoter

   Depositor

   David Liu

   Article

   Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

   Insert

   Npu-saBE3.9max_N-terminal
   

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Promoter

   CMV

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Frt-V5-hspB8

   Plasmid

   #63109

   Purpose

   Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb8 (HSPB8 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. 2xmyc-JIP4

    Plasmid

    #164633

    Purpose

    Expresses JIP4 in mammalian cells with a 2xmyc tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    JIP4 (SPAG9 Human)
    

    Tags

    2xmyc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Frt-hspB5

    Plasmid

    #63096

    Purpose

    Expression of HSPB5 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb5 (CRYAB Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. Frt-V5-hspB5

    Plasmid

    #63106

    Purpose

    Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb5 (CRYAB Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. p3xFLAG-CMV14/p14ARF

    Plasmid

    #78760

    Purpose

    To overexpress p14ARF in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

    Insert

    ARF (CDKN2A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

14. miniCGBE1-NG (pBM1182)

    Plasmid

    #140257

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TadA-NG-CBE

    Plasmid

    #227420

    Purpose

    C-to-T base editing in mammalian cells

    Depositor

    Yang Sun

    Article

    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d

    Insert

    TadA-nSpCas9-NG-2xUGI (AICDA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. mMib1-FLAG

    Plasmid

    #37116

    Depositor

    Martin Dorf

    Article

    Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

    Insert

    Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)
    

    Tags

    3 x FLAG

    Expression

    Mammalian

    Mutation

    ACC Kozak sequence added before ATG annotated.

    Promoter

    CMV

    Available Since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. LLP773_pLenti-6xHRBKET-sgRNA

    Plasmid

    #211766

    Purpose

    Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
    

    Use

    Lentiviral

    Promoter

    pCMV and U6

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA_B2AR-s-pep

    Plasmid

    #47438

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

19. IL2R/hE-cadherin-cytotail

    Plasmid

    #45773

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)
    

    Expression

    Mammalian

    Mutation

    Interleukin-2 receptor α subunit extracellular an…

    Promoter

    CMV

    Available Since

    June 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. miniCGBE1-VRQR (pBM1173)

    Plasmid

    #140255

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_VRQR-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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