We narrowed to 9,512 results for: try

1. STK24_HUMAN_D0

   Plasmid

   #79742

   Purpose

   This plasmid encodes the kinase domain of STK24. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Markus Seeliger, Nicholas Levinson

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   STK24 (STK24 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pEN_TT 3xFLAG-KPNA2

   Plasmid

   #192306

   Purpose

   Gateway entry vector for an inducible 3xFLAG-KPNA2

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   KPNA2 (KPNA2 Human)
   

   Use

   Gateway entry vector

   Tags

   3xFLAG

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNSSCP

   Plasmid

   #183188

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   DivIVA and superfolder GFP fusion protein
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiGuide-Puro-IFITM3g1 (BB15)

   Plasmid

   #139460

   Purpose

   Lentiviral vector with gRNA targeting IFITM3; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   IFITM3-targeting sgRNA inserted; resistance gene: puroR (IFITM3 Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF-1a / U6

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAC-TMPRSS2

   Plasmid

   #158385

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 26, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDONR221-ARL8B

   Plasmid

   #67334

   Purpose

   hArl8b with stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8B (ARL8B Human)
   

   Use

   Gateway entry vector

   Expression

   Bacterial

   Promoter

   None

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pENTR221-GLI1

   Plasmid

   #79497

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human GLI1 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   GLI1 (GLI1 Human)
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-CYLD-C601A

   Plasmid

   #60028

   Purpose

   N-terminal GFP tagged catalytic inactive (C601A) expression

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

   Insert

   Cylindromatosis (CYLD Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Cysteine 601 changed to Alanine

   Promoter

   CMV

   Available Since

   Nov. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ239

   Plasmid

   #108859

   Purpose

   FnCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

   Insert

   FnCpf1
   

   Use

   CRISPR; Gateway compatible cpf1 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Cpf1 is rice codon optimized

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE-Tight-FLAG-CYLD-C601A

    Plasmid

    #60029

    Purpose

    CYLD (C601A) catalytic dead under control of Tet promoter with N-terminal FLAG tag

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Cysteine 601 changed to Alanine

    Promoter

    TRE

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. KQ403: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-LSD1

    Plasmid

    #121831

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLSx-dCas9(3.7) fused to the LSD1 H3K4me1/2 demethylase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/LSD1 (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. 374 pME-ets1a

    Plasmid

    #49000

    Purpose

    full length coding sequence for zebrafish ets1a gene in gateway middle entry vector

    Depositor

    Nathan Lawson

    Article

    Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

    Insert

    v-ets erythroblastosis virus E26 oncogene homolog 1a (ets1 Zebrafish)
    

    Use

    Gateway middle entry

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. SLK_HUMAN_D0

    Plasmid

    #79733

    Purpose

    This plasmid encodes the kinase domain of SLK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SLK (SLK Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pYPQ265G2

    Plasmid

    #164723

    Purpose

    Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing

    Depositor

    Yiping Qi

    Article

    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

    Insert

    A3A(Y130F)-ziSpyMac(D10A)-UGI)
    

    Use

    CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…

    Expression

    Plant

    Available Since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. (p)odr-3::GFP::EGL-4

    Plasmid

    #100897

    Purpose

    GFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.

    Depositor

    Noelle L'Etoile

    Article

    Lee et al Proc Natl Acad Sci U S A. 2010 Mar 30;10

    Insert

    56-2741: odr-3 promoter
    

    Expression

    Worm

    Promoter

    odr-3

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKK-HA-TEV

    Plasmid

    #105769

    Purpose

    Expression of your protein of interest in fusion with HA at the N-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    HA-TEV

    Expression

    Mammalian

    Available Since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ165

    Plasmid

    #109327

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    Promoter EC1.2 enhancer fused to EC1.1 promoter

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKK-TEV-HA

    Plasmid

    #105785

    Purpose

    Expression of your protein of interest in fusion with HA at the C-terminus. The tag is cleavable by TEV protease.

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-HA

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST47-CYFIP2.GFP

    Plasmid

    #109140

    Purpose

    GFP-tagged CYFIP2 expression construct

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. 139H2_LC

    Plasmid

    #206202

    Purpose

    For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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