We narrowed to 5,300 results for: pcDNA3.1+

1. CMV-SpyCas9

   Plasmid

   #113033

   Purpose

   SpyCas9 expression in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SpyCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-Hs.cargo(Cre)

   Plasmid

   #174863

   Purpose

   Expresses human Peg10 cargoRNA encoding Cre recombinase

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   Cre flanked by HsPeg10 UTRs
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. HaloTag-ER-3

   Plasmid

   #186960

   Purpose

   ER lumen localizaing HaloTag

   Depositor

   Ke Xu

   Article

   Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

   Insert

   CALR (CALR Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Flag-H3.3-polyA

   Plasmid

   #59780

   Purpose

   Express Flag-tagged H3.3 (H3F3B) in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

   Depositor

   Yi Zhang

   Article

   Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

   Insert

   H3F3B (H3-3B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. FRB-NLuc

   Plasmid

   #31181

   Depositor

   David Piwnica-Worms

   Article

   Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

   Insert

   Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-V1-ORF

   Plasmid

   #73635

   Purpose

   Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Use

   Tags

   Venus fragment 1 (V1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-V2-ORF

   Plasmid

   #73636

   Purpose

   Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

   Depositor

   Darren Saunders

   Article

   Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

   Type

   Empty backbone

   Use

   Tags

   Venus fragment 2 (V2)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. L2383-FRB-GPA-NLuc

   Plasmid

   #222902

   Purpose

   Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

   Depositor

   Joshua Leonard

   Article

   Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

   Insert

   FRB-GPA-20GS-NanoLuc
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Mfn1-Myc

   Plasmid

   #23212

   Depositor

   David Chan

   Article

   Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

   Insert

   Mfn1 (Mfn1 Mouse)
   

   Use

   Tags

   6xHis, 9xMyc, and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. HaloTag-sec61b

    Plasmid

    #186959

    Purpose

    Express sec61b connected with N-terminus HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Sec61 (SEC61B Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. qMaLioffG

    Plasmid

    #212825

    Purpose

    green fluorescent FLIM indicator for endogenous ATP imaging

    Depositor

    Tetsuya Kitaguchi

    Article

    Arai et al bioRxiv 2023.08.29.555275

    Insert

    qMaLioffG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Human Integrin beta 2

    Plasmid

    #8640

    Depositor

    Timothy Springer

    Article

    Kishimoto et al Cell 1987 Feb 27;48(4):681-90.

    Insert

    Integrin beta 2 (ITGB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

13. MCP/PCP RNA Lantern

    Plasmid

    #226095

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-NES-PhoCl-mCherry

    Plasmid

    #87690

    Purpose

    Express NES-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NES-PhoCl-mCherry
    

    Use

    Tags

    nuclear export signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmyc-GFP-TNRC6A-(1-1223)

    Plasmid

    #42013

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    contain aa1-1223

    Promoter

    CMV

    Available since

    March 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. Vinculin-venus

    Plasmid

    #27300

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    Venus A206K

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDEST-ORF-V2

    Plasmid

    #73638

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 2 (V2).

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 2 (V2)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-ORF-V1

    Plasmid

    #73637

    Purpose

    Gateway™ destination vector encoding C-terminal fusion with Venus fluorescent protein fragment 1 (V1)

    Depositor

    Darren Saunders

    Article

    Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

    Type

    Empty backbone

    Use

    Tags

    Venus fragment 1 (V1)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Red Glifon Nega

    Plasmid

    #163117

    Purpose

    Red fluorescent protein-based glucose negative control sensor (does not respond to glucose) for mammalian cells

    Depositor

    Takashi Tsuboi

    Article

    Mita et al Cell Chem Biol. 2021 Jun 29. pii: S2451-

    Insert

    Red Glifon Nega
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Venus-155-239-Gbeta1

    Plasmid

    #234751

    Purpose

    Mammalian expression of BiFC Venus-betagamma (with Venus-1-155-Ggamma2)

    Depositor

    Nevin Lambert

    Article

    Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

    Insert

    GNB1 (GNB1 Human)
    

    Use

    Tags

    VN155

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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