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pJexpress414_OmpA171_R4cons

Plasmid

#62639

Purpose

Conservative version of OmpA Redesign 4

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_R4cons

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Mutation

25 mutations to lipid-facing surface

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pSR14.483

Plasmid

#39544

Depositor

Katharina Strub

Article

Lakkaraju et al Cell. 2008 May 2;133(3):440-51.

Insert

SRP14 (SRP14 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

H1

Available Since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDEST17_A1LigN_EC

Plasmid

#22296

Depositor

Oscar Millet

Article

Tadeo et al PLoS Biol. 2009 Dec;7(12):e1000257. doi:

Insert

A1 domain NAD+ dependent DNA ligase N

Tags

His tag

Expression

Bacterial

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only
pNORg10

Plasmid

#249340

Purpose

Mediterraneibacter gnavus - Escherichia coli shuttle vector with catP selection marker

Depositor

Nozomu Obana

Article

Obana et al Nat Commun. 2026 Mar 12. doi: 10.1038/s4

Type

Empty backbone

Use

E. coli - r. gnavus shuttle vector

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_lacI_Plac

Plasmid

#243920

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI and PlacZYA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_tetR_Ptet

Plasmid

#243924

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_cI857_PR

Plasmid

#243926

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7

Plasmid

#243928

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_PARTPRM_PT7lac

Plasmid

#243929

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, not including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_lacI_Plac

Plasmid

#243909

Purpose

GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

lacI and PlacZYA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_araC_Para

Plasmid

#243911

Purpose

GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

araC and ParaBAD

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_tetR_Ptet

Plasmid

#243913

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_cI857_PR

Plasmid

#243915

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

cI857 and PR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PT7

Plasmid

#243917

Purpose

GoldenBraid2.0 T7 RNA polymerase-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PT7lac

Plasmid

#243918

Purpose

GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent promoter, including ribosome binding site.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PT7lac

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKo1_Stuffer1_Stuffer2

Plasmid

#243896

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer1 and Stuffer2

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBSKo2_Stuffer2_Stuffer1

Plasmid

#243897

Purpose

GoldenBraid2.0 Full transcription unit stuffer in an omega-level cloning vector.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

Stuffer2 and Stuffer1

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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ptetgRNA-FRT

Plasmid

#243872

Purpose

sgRNA expression vector under tetR transcriptional control.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

sgRNA_FRT

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pDQM139

Plasmid

#247638

Purpose

to generate mRNA for membrane mGold2t for zebrafish

Depositor

Gokul Upadhyayula

Article

Tschanz et al MicroPubl Biol. 2025 Nov 25;2025:10.1791

Insert

2x Lyn Kinase membrane localization sequence

Use

Zebrafish mrna expression using sp6

Tags

mGold2t

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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ISA179: Tagatose pathway

Plasmid

#241968

Purpose

Phagemid for the tagatose pathway. Contains p15A, CmR, pac site, AraC, C1, and pBAD.

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

Tagatose pathway

Use

Synthetic Biology; Phagemid

Expression

Bacterial

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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