We narrowed to 24,507 results for: Spr;

1. pBBK84 pHomL-RPL18Bp-PDR16-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179068

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of PDR16-Link-Scarlet(rfp), a marker for the lipid droplet

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-PDR16-Link-Scarlet(rfp)
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBBK79 pHomL-RPL18Bp-Scarlet(rfp)-Link-YPT7-SSA1t-HomR (AmpR)

   Plasmid

   #179063

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-YPT7, a marker for the vacuole, late endosome

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-Scarlet(rfp)-Link-YPT7
   

   Expression

   Yeast

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. G1397 DddAtox-C–SaKKH-Cas9(D10A)

   Plasmid

   #157840

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   CRISPR

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PX458_KDM1A_1

   Plasmid

   #86297

   Purpose

   Encodes gRNA for 3' target of human KDM1A

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against KDM1A (KDM1A Human)
   

   Use

   CRISPR

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-sgGAL4-1

   Plasmid

   #46915

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Inserts

   sgGAL4-1
   Puromycin resistance and mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CMV and U6

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458_KDM1A_2

   Plasmid

   #86298

   Purpose

   Encodes gRNA for 3' target of human KDM1A

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against KDM1A (KDM1A Human)
   

   Use

   CRISPR

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. FEN1 C10.3 gRNA

   Plasmid

   #90689

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FEN1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   FEN1 (Guide Designation C10.3)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS-u(S)Cas9

   Plasmid

   #127383

   Purpose

   Expresses Cas9 at high level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(S)-Cas9
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   UAST

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB_EF1a-CasRx-msfGFP-2A-Blast

   Plasmid

   #226010

   Purpose

   CasRx protein for RNA targeting in piggyBac backbone

   Depositor

   Silvana Konermann

   Article

   Wei et al Cell Syst. 2023 Dec 20;14(12):1087-1102.

   Insert

   CasRx RNA-targeting nuclease
   

   Use

   CRISPR

   Tags

   msfGFP

   Expression

   Mammalian

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHelper-T7IS1

    Plasmid

    #140631

    Purpose

    Expresses ShCAST under the T7 promoter and an empty sgRNA

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA targeting IS1 (Escherichia coli)
    

    Use

    CRISPR; Transposon

    Expression

    Bacterial

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT3TS-zAsCpf1

    Plasmid

    #107185

    Purpose

    plasmid to carry out IVT of AsCpf1 (zebrafish codon optimized)

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    AsCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. dpYPQ230-D156R-CBE1

    Plasmid

    #195367

    Purpose

    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
    

    Use

    CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

    Tags

    3xflag

    Expression

    Plant

    Promoter

    ZmUBI1

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV_eA3A-nCas9

    Plasmid

    #163535

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SMC6 G4.4 gRNA

    Plasmid

    #90899

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G4.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. SMC6 G3.4 gRNA

    Plasmid

    #90898

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SMC6

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SMC6 (Guide Designation G3.4)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSP64T-hLbCpf1

    Plasmid

    #107187

    Purpose

    plasmid to carry out IVT of human codon optimized LbCpf1 (based on Addgene 69988)

    Depositor

    Antonio Giraldez

    Article

    Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

    Insert

    hLbCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Promoter

    sp6

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-eGFP-dSaCas9-blast

    Plasmid

    #191653

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

    Insert

    eGFP-T2A-dSaCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)

    Plasmid

    #179064

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuole

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-Scarlet(rfp)-Link-VAM3
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. MiniCoopR U6:gRNA p53, mitfa:Cas9

    Plasmid

    #118841

    Purpose

    Targets tp53 and expresses zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    tp53 gRNA (tp53 Zebrafish)
    

    Use

    CRISPR; Tol2

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRW004-SM-destination-proUBQ10-Cas9

    Plasmid

    #104438

    Purpose

    Destination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;

    Depositor

    Detlef Weigel

    Article

    Wu et al Plant Methods. 2018 Aug 2;14:65. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial and Plant

    Promoter

    UBQ10

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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