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pENTR-D-TOPO-PAmCherry1_1-159-MCS

Plasmid

#60545

Purpose

Cloning PAmCherry1 N-terminal fragment (RN) for BiFC-PALM

Depositor

Xiaolin Nan

Article

Nickerson et al PLoS One. 2014 Jun 25;9(6):e100589. doi:

Insert

PAmCherry1 N-terminal fragment

Use

Cloning

Mutation

amino acids 1-159

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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MKK7

Plasmid

#29576

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK7 (Map2k7 Mouse)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Starting Met removed

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
OA-1050J (GFP)

Plasmid

#133304

Purpose

express arrays of gRNA targeting GFP under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

GFP gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pKF-P14MM2AG5h

Plasmid

#128254

Purpose

Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

rtTA-Advanced::P2A::EGFP

Use

Synthetic Biology; Flp-in expression vector

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-Nedd4-N / 2524 JM16

Plasmid

#13312

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

Nedd4 (Nedd4 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa1-52 and aa422-777 deleted

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-Y1699C

Plasmid

#25084

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Destination vector

Tags

6XHis and V5

Expression

Mammalian

Mutation

Tyrosine 1699 mutated to Cysteine

Available Since

July 2, 2010

Availability

Academic Institutions and Nonprofits only
pET15-MTFmt

Plasmid

#31174

Depositor

Linda Spremulli

Article

Takeuchi et al J Biol Chem. 1998 Jun 12. 273(24):15085-

Insert

Mature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)

Tags

His and thrombin cleavage

Expression

Bacterial

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pBad33_ISMmu1_reRNA

Plasmid

#205349

Purpose

Bacterial expression of ISMmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISMmu1 TnpB

Expression

Bacterial

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf9

Plasmid

#163599

Purpose

Expresses GDF-9 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf9 (Gdf9 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Kohinoor/pRSETb

Plasmid

#67770

Purpose

Kohinoor for bacterial expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pBad33_ISAba30_reRNA

Plasmid

#205323

Purpose

Bacterial expression of ISAba30 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISAba30 TnpB

Expression

Bacterial

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCR2.1 mNFATc2

Plasmid

#17858

Depositor

Jerry Crabtree

Article

Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

Insert

NFATc2 (Nfatc2 Mouse)

Expression

Bacterial

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
pRetroSuper-GFP TIF1gamma

Plasmid

#15721

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma shRNA (TRIM33 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
Kohinoor-Paxillin/pcDNA3

Plasmid

#67775

Purpose

Paxilin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Paxillin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pLNCX Myr His p110 KD

Plasmid

#10837

Depositor

Thomas Roberts

Article

Auger et al Biochem Biophys Res Commun. 2000 Jun 16.

Insert

p110 KD (PIK3CA Bovine)

Use

Retroviral

Tags

6xHis and Myr

Expression

Mammalian

Mutation

D915S. Kinase Dead.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Smad3 LC

Plasmid

#12633

Depositor

Rik Derynck

Article

Zhang et al Nature. 1998 Aug 27. 394(6696):909-13.

Insert

Smad3 LC (SMAD3 Human)

Tags

GST

Expression

Bacterial

Mutation

LC segment of Smad3 (aa 146-425)

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pDiySpike1

Plasmid

#136948

Purpose

Plasmid for the In Vitro transcription of DiySpike1

Depositor

Rickard Sandberg

Article

Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

Insert

DiySpike1

Use

Synthetic Biology

Promoter

T7

Available Since

April 14, 2020

Availability

Academic Institutions and Nonprofits only

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Lyn-tdnano-HA

Plasmid

#136512

Purpose

Mammalian expression of a tandem dimer of WT SspB (nano), targeted to membrane by Lyn myristoylation sequence, with triple-HA epitope tag

Depositor

Bianxiao Cui

Article

Hope et al J Mol Biol. 2020 Jun 12;432(13):3739-374

Insert

Lyn nano nano 3HA

Expression

Mammalian

Promoter

CMV

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

Plasmid

#185687

Purpose

Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-GST-2xHaloTag-KKMP

Expression

Mammalian

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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