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pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

Plasmid

#172316

Purpose

CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

Depositor

Steven Jacobsen

Article

Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGC(2.1)-U6.3

Plasmid

#170516

Purpose

PCR template vector for amplifying gRNAcore(2.1)-pU6.3 promoter fragment; used together with pAC-CR7T-gRNA2.1-nlsBFP

Depositor

Chun Han

Article

Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

Insert

gRNAcore(2.1)-pU6.3

Use

Pcr template vector for amplifying grnacore(2.1)-…

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

Plasmid

#114112

Purpose

fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

NanoLuc; Volvox channelrhodopsin 1; EYFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ERBB2 G309E

Plasmid

#40990

Depositor

Matthew Meyerson

Article

Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

Insert

ERBB2 (ERBB2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G309E

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pACT2-HsMCU-Flag

Plasmid

#253496

Purpose

Flag tagged HsMCU in pACT2 vector

Depositor

Vamsi Mootha

Article

Kovacs-Bogdan et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

HsMCU CD (MCU Human)

Tags

Flag

Expression

Bacterial and Yeast

Available Since

May 18, 2026

Availability

Academic Institutions and Nonprofits only

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co LuxDEC(KZK)

Plasmid

#253533

Purpose

Express luciferin biosynthesis of human codon-optimized LuxDEC in mammalian cells

Depositor

Takeharu Nagai

Article

Kusuma et al Proc Natl Acad Sci U S A. 2024 Oct 8;121

Insert

co LuxD-P2A-co LuxE-T2A-co LuxC

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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co LuxB-Frp (G4S)

Plasmid

#253532

Purpose

Express human codon-optimized of LuxB-G4S-Frp in mammalian cells

Depositor

Takeharu Nagai

Article

Kusuma et al Proc Natl Acad Sci U S A. 2024 Oct 8;121

Insert

co LuxB-Frp(G4S)

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2026

Availability

Academic Institutions and Nonprofits only

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pRsetB-TEV-PiTq

Plasmid

#248768

Purpose

Bacterial expression vector for PiTq

Depositor

Gary Yellen

Article

Rosen et al Proc Natl Acad Sci U S A. 2026 Mar 31;12

Insert

PiTq

Expression

Bacterial

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m-V5

Plasmid

#251979

Purpose

For biolistic transformation to express Arg8m from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

mtArg8m

Tags

V5

Expression

Yeast

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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pPT24-mtArg8m*-V5

Plasmid

#251978

Purpose

For biolistic transformation to express Arg8m* from mtDNA

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

mtArg8m*

Tags

V5

Expression

Yeast

Mutation

premature stop CAA->TAA, Q79*

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti Blast GRP94 WT V5

Plasmid

#240446

Purpose

Expresses GRP94 in mammalian cells

Depositor

Michael Kastan

Article

Burrell et al Proc Natl Acad Sci U S A. 2025 Dec 30;12

Insert

GRP94 (HSP90B1 Human)

Use

Lentiviral

Tags

V5 tag and second KDEL sequence

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti Blast GRP94 S64A V5

Plasmid

#240447

Purpose

Expresses mutant GRP94 in mammalian cells

Depositor

Michael Kastan

Article

Burrell et al Proc Natl Acad Sci U S A. 2025 Dec 30;12

Insert

GRP94 (HSP90B1 Human)

Use

Lentiviral

Tags

V5 tag and second KDEL seuence

Expression

Mammalian

Mutation

changed serine 64 to alanine

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti Blast GRP94 S64 D V5

Plasmid

#240448

Purpose

Expresses mutant GRP94 in mammalian cells

Depositor

Michael Kastan

Article

Burrell et al Proc Natl Acad Sci U S A. 2025 Dec 30;12

Insert

GRP94 (HSP90B1 Human)

Use

Lentiviral

Tags

V5 tag and second KDEL sequence

Expression

Mammalian

Mutation

changed serine 64 to aspartate

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti Blast GRP94 N62Q V5

Plasmid

#240449

Purpose

Expresses mutant GRP94 in mammalian cells

Depositor

Michael Kastan

Article

Burrell et al Proc Natl Acad Sci U S A. 2025 Dec 30;12

Insert

GRP94 (HSP90B1 Human)

Use

Lentiviral

Tags

V5 tag and second KDEL sequence

Expression

Mammalian

Mutation

changed asparagine 62 to glutamine

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pBL155c

Plasmid

#246592

Purpose

express cCadC NtRbcL fusion protein

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

cCadC-NtRbcL (rbcL Synthetic)

Expression

Bacterial

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pAH_DEST_nHalo-Sec61β-3XFLAG

Plasmid

#250996

Purpose

Expression of Sec61β with N-terminal Halo and C-terminal 3XFLAG tags

Depositor

Anthony Pedley

Article

Sha et al Proc Natl Acad Sci U S A. 2026 Feb 3;123

Insert

SEC61β (SEC61B Human)

Tags

3XFLAG and Halo Tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pSL012

Plasmid

#246580

Purpose

express mGreen Lantern from the cadBA promoter

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

mGreenLantern

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pTW368z

Plasmid

#246581

Purpose

Golden gate acceptor for AtRaf1 library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

sfGFP, AtRaf2, AtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (RbcX2, CPN60B, CPN20, CPN60A Mustard Weed)

Expression

Bacterial

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pSYL018

Plasmid

#246594

Purpose

template plasmid for site-saturation mutagenesis library cloning

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

AtRaf1 variant 2b (AT3G04550 Mustard Weed)

Expression

Bacterial

Mutation

AtRaf1I90V/I127F/R139H/M243K/E275V/V334A/K411I/R4…

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pBL155

Plasmid

#246591

Purpose

express cCadC AtRbcL fusion protein

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

cCadC-AtRbcL (rbcL Synthetic)

Expression

Bacterial

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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