We narrowed to 44,105 results for: cha

1. pAAV-hSyn-ASAP3Kv-WPRE

   Plasmid

   #132332

   Purpose

   expresses ASAP3Kv(soma-located version of ASAP3, ASAP-family genetically encoded voltage indicator) in mammalian cells, gene delivered by pAAV-hSyn vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3Kv
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-ASAP3-WPRE

   Plasmid

   #132318

   Purpose

   expresses ASAP3(ASAP-family genetically encoded voltage indicator) in cre recombinase expressing mouse/cell, gene delivered by pAAV-EF1a vector

   Depositor

   Michael Lin

   Article

   Villette et al Cell. 2019 Dec 12;179(7):1590-1608.e23.

   Insert

   ASAP3
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCUabaa-Flag-PLYS1

   Plasmid

   #236786

   Purpose

   MCU-MCUB fusion in abaa pattern. Only the initial MCU has a mitochondrial targeting sequence.

   Depositor

   Dipayan Chaudhuri

   Article

   Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

   Insert

   MCUB, MCU mixed tetramer (MCU, MCUB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. MCUabab-Flag-pLYS1

   Plasmid

   #236788

   Purpose

   MCU-MCUB fusion in abab pattern. Only the initial MCU has a mitochondrial targeting sequence.

   Depositor

   Dipayan Chaudhuri

   Article

   Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

   Insert

   MCUB, MCU mixed tetramer (MCU, MCUB Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLgw EcoDam-V5-ELK1

   Plasmid

   #98604

   Purpose

   Mammalian DamID lentiviral vector for ELK1 with Dam-V5 using Gateway cloning

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Lentiviral; Damid

   Tags

   Dam (DNA adenine methyltransferase) and V5

   Expression

   Mammalian

   Mutation

   Promoter

   Heat Shock Minimal Promoter

   Available Since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP Cav2.2 HA D122A pcDNA3

   Plasmid

   #206120

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D122A

   Promoter

   CMV

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cav2.2 HA E1658A (EIVA) pcDNA3

   Plasmid

   #206114

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658A

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cav2.1 EIVA (E1707A) pcDNA3

   Plasmid

   #206125

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1707A

   Promoter

   CMV

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav2.1 HA EIA (E320A) pcDNA3

   Plasmid

   #206124

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E320A

   Promoter

   CMV

   Available Since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 SIRT1-deltaExon2

    Plasmid

    #105671

    Purpose

    Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    CMV

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MCUabbb-Flag-PLYS1

    Plasmid

    #236787

    Purpose

    MCU-MCUB fusion in abbb pattern. Only the initial MCU has a mitochondrial targeting sequence.

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB, MCU mixed tetramer (MCU, MCUB Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDual

    Plasmid

    #207883

    Purpose

    Expresses thermosensitive variants of the homing endonucleases I-OnuI and I-GpeMI, each in a different open reading frame. Both were made thermosensitive by a fusion with the L212P VMA1 intein.

    Depositor

    David Edgell

    Article

    Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

    Inserts

    Thermosensitive fusion of I-OnuI and the L212P VMA1 intein.
    Thermosensitive fusion of I-GpeMI and the L212P VMA1 intein.
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Leucine 212 changed to proline, leucine 434 in th…

    Promoter

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cav2.2 HA EIVA (E1658A) pCAGGS

    Plasmid

    #206082

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658A

    Promoter

    CMV/B-actin

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Cav2.2 HA EIA (E314A) pCAGGS

    Plasmid

    #206081

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E314A

    Promoter

    CMV/B-actin

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid

    #206066

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Cav2.2 HA EIVK (E1658K) pCAGGS

    Plasmid

    #206096

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV/B-actin

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4 TAPBPR-TM-TN6

    Plasmid

    #178650

    Purpose

    Mammalian expression of FLAG-tagged TAPBPR with MHC TM (E205K, R207E, Q209S, Q272S)

    Depositor

    Erik Procko

    Article

    McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

    Insert

    TAPBPR (TAPBPL Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    E205K, R207E, Q209S, Q272S, switched transmembran…

    Promoter

    CMV

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEX-4T-3 SIRT1-NTerm Exon1-Exon3

    Plasmid

    #105681

    Purpose

    Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon3 (deltaExon2)

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1-NTerm: Exon1-Exon3 (Sirt1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    tac_Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T-3- SIRT1-NTerm Exon1-Exon2-Exon3

    Plasmid

    #105680

    Purpose

    Bacterial Expression construct of N terminus of SIRT1: Exon1-Exon2-Exon3

    Depositor

    Ullas Kolthur-Seetharam

    Article

    Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

    Insert

    SIRT1-NTerm: Exon1-Exon2-Exon3 (Sirt1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Synonymous base changes not affecting the protein…

    Promoter

    tac_Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLgw EcoDam-V5-NKX2-5Y191C

    Plasmid

    #98598

    Purpose

    Mammalian DamID lentiviral vector for fusing NKX2-5 Y191C with Dam-V5 using Gateway cloning

    Depositor

    Richard Harvey

    Article

    Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

    Insert

    NKX2-5 (Nkx2-5 Mouse)
    

    Use

    Lentiviral; Damid

    Tags

    Dam (DNA adenine methyltransferase) and V5

    Expression

    Mammalian

    Mutation

    Y191C point mutant

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge