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pHFX-B60-EPSUD

Plasmid

#174390

Purpose

Plasmids for gene knockout were constructed based on suicide plasmid pHFX-B60

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

EPSUD

Use

Archaeal gene knockout vector for haloferax medit…

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-IV

Plasmid

#53438

Purpose

xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pMJA053

Plasmid

#128515

Purpose

Expresses SFA8 in Pichia

Depositor

Marcos Alcocer

Article

Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

Insert

SFA8 a 2S albumin from sunflower (LOC110892047 Helianthus annus)

Expression

Yeast

Promoter

AOX1

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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GST-Cav (61-101)

Plasmid

#22447

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin1 (61-101)

Tags

GST

Expression

Bacterial

Available Since

Jan. 26, 2010

Availability

Academic Institutions and Nonprofits only
GST-Cav (135-178)

Plasmid

#22448

Depositor

William Sessa

Article

Garcia-Cardena et al J Biol Chem. 1997 Oct 10. 272(41):25437-

Insert

Caveolin 1

Tags

GST

Expression

Bacterial

Mutation

aa 135-178

Available Since

Feb. 4, 2010

Availability

Academic Institutions and Nonprofits only
pUAP1_Ta_Yr7_CDS_no_STOP

Plasmid

#141093

Purpose

Level 0 Golden gate module with the coding sequence of Cadenza-Yr7 without its STOP codon

Depositor

Cristobal Uauy

Article

Marchal et al Genes (Basel). 2020 Nov 26;11(12). pii:

Insert

Ta_Yr7

Use

Unspecified; Level 0 module for golden gate cloni…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pDMG8a

Plasmid

#33179

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

C27H6.9 3'UTR (C27H6.9 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pDMG1a

Plasmid

#33158

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

cog-1 3'UTR (cog-1 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pAG249

Plasmid

#33202

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

WBP4 3'UTR (WBP4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pSuper-TatSF1 GH

Plasmid

#31120

Depositor

Mariano Garcia-Blanco

Article

Miller et al PLoS One. 2009 . 4(5):e5710.

Insert

TatSF1 shRNA (HTATSF1 Human)

Use

RNAi

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pSuper-TatSF1 AB

Plasmid

#31119

Depositor

Mariano Garcia-Blanco

Article

Miller et al PLoS One. 2009 . 4(5):e5710.

Insert

TatSF1 shRNA (HTATSF1 Human)

Use

RNAi

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pUE001-Dendra2Hfx

Plasmid

#234669

Purpose

encodes Dendra2Hfx in Haloferax volcanii under the tryptophan inducible p.tna promoter, trpA, ampR, shuttle vector for E.coli

Depositor

Ulrike Endesfelder

Article

Schrage et al μLife

Insert

Dendra2Hfx

Use

Archaeal expression in haloferax volcanii

Tags

Dendra2Hfx

Promoter

p.tna

Available Since

March 11, 2026

Availability

Academic Institutions and Nonprofits only

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pTIR_HyPB_RFP

Plasmid

#248201

Purpose

HyPB RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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pTIR_Antgra4_RFP

Plasmid

#248203

Purpose

Antgra4 RFP cargo plasmid

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

RFP

Expression

Mammalian

Available Since

Jan. 12, 2026

Availability

Academic Institutions and Nonprofits only

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excision_reporter_HyPB

Plasmid

#248200

Purpose

HyPB and synthetic transposase excision reporter and targeted integration reporter

Depositor

Marc Güell

Article

Ivancic et al Nat Biotechnol. 2025 Oct 2. doi: 10.1038

Insert

Excision RFP reporter

Expression

Mammalian

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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Thor-LSSM13-A

Plasmid

#245864

Purpose

Archael (13cm_4210_3138-4177(-) _Thor) 16S rRNA expression

Depositor

Roland Hatzenpichler

Article

Van Beek et al Microbiol Resour Announc. 2025 Dec 11;14

Insert

16S rRNA

Use

Other

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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MlumB10

Plasmid

#245863

Purpose

Archael (Methanomassiliicoccus luminyensis B10) 16S rRNA expression

Depositor

Roland Hatzenpichler

Article

Van Beek et al Microbiol Resour Announc. 2025 Dec 11;14

Insert

16S rRNA

Use

Other

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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Nitgar

Plasmid

#245859

Purpose

Archael (Nitrososphaera gargensis RHGA36) 16S rRNA expression

Depositor

Roland Hatzenpichler

Article

Van Beek et al Microbiol Resour Announc. 2025 Dec 11;14

Insert

16S rRNA

Use

Other

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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LCB70

Plasmid

#245856

Purpose

Archael (Methanosuratincola verstraetei LCB70) 16S rRNA expression

Depositor

Roland Hatzenpichler

Article

Van Beek et al Microbiol Resour Announc. 2025 Dec 11;14

Insert

16S rRNA

Use

Other

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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LCB24

Plasmid

#245855

Purpose

Archael (Methanoglobus hypatiae LCB24) 16S rRNA expression

Depositor

Roland Hatzenpichler

Article

Van Beek et al Microbiol Resour Announc. 2025 Dec 11;14

Insert

16S rRNA

Use

Other

Available Since

Oct. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBWB507

Plasmid

#209325

Purpose

p15A-tetR-tet-ME-RBS-mRFP-ME-U1-Barcode-U2-dbI terminator-oriT-Cm. Plasmid backbone for fragment expression.

Depositor

Adam Arkin

Article

Biggs et al Mol Syst Biol. 2024 Oct 7. doi: 10.1038/

Insert

mRFP

Expression

Bacterial

Promoter

tet

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_pNOS-Rep-RepA-Tnos (GB3598)

Plasmid

#225432

Purpose

Transcriptional unit of BeYDV Rep/RepA (C1:C2)

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

pNOS:Rep/RepA:tNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-2H12Matry

Plasmid

#221187

Purpose

His6-tagged cdGreen-Matry expressed from T7 promoter (Internal Lab ID: UJ11309)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen-Matry

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pZG164

Plasmid

#202583

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-hptII

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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FB395

Plasmid

#203631

Purpose

TU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1aG2b.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB396

Plasmid

#203632

Purpose

TU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1ab.1:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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FB397

Plasmid

#203633

Purpose

TU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

R1:G1abc.3:mPAF:Luc:TtrpC

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW2-MaUPPS-B

Plasmid

#203175

Purpose

Yeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

MA_4402

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pKG-GW1-MaUPPS-A

Plasmid

#203174

Purpose

Yeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase

Depositor

William Sessa

Article

Grabinska et al J Biol Chem. 2017 Oct 20;292(42):17351-1

Insert

uppS

Expression

Yeast

Promoter

TDH3

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pZG161

Plasmid

#202580

Purpose

RNA editing in plants. Plasmid contains extradiol-dADARcd-LIC cassette-dADARcd-35S-bar

Depositor

Guoyong Xu

Article

Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

Insert

HyperADARcd

Expression

Plant

Mutation

E488Q

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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