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pHAGE-mEGFP-hCEBPa_AroLITE

Plasmid

#215614

Purpose

For integration of CEBPa AroLITE mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroLITE (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NGN2-WT

Plasmid

#215607

Purpose

For overexpression of Gal4-DBD NGN2 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NGN2 (NEUROG2 Human)

Expression

Mammalian

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXC4-AroPERFECT

Plasmid

#215588

Purpose

For overexpression of Gal4-DBD HOXC4 AroPERFECT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXC4 AroPERFECT (HOXC4 Human)

Expression

Mammalian

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXC4-AroLITE_S

Plasmid

#215587

Purpose

For overexpression of Gal4-DBD HOXC4 AroLITE S

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

HOXC4 AroLITE S (HOXC4 Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-NANOG-AroLITE_A

Plasmid

#215600

Purpose

For overexpression of Gal4-DBD NANOG AroLITE A

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NANOG AroLITE A (NANOG Human)

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_AroPERFECT_IS15

Plasmid

#215615

Purpose

For integration of CEBPa AroPERFECT IS15 mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS15 (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_AroPERFECT_IS10

Plasmid

#215616

Purpose

For integration of CEBPa AroPERFECT IS10 mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa AroPERFECT IS10 (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-mEGFP-hCEBPa_WT

Plasmid

#215613

Purpose

For integration of CEBPa WT mEGFP

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

CEBPa (CEBPA Human)

Use

Lentiviral

Tags

mEGFP

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot9-V181E-shRNA2res_AG

Plasmid

#148829

Purpose

Mammalian Expression of HsNot9-V181E-shRNA2res

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot9-V181E-shRNA2res (CNOT9 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-HsNot10_25-707-HsNot11_257-498-CvH_AF

Plasmid

#148731

Purpose

Bacterial Expression of HsNot10_25-707-HsNot11_257-498

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

HsNot10_25-707-HsNot11_257-498 (CNOT10 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-ABL1-gRNA1-exon4

Plasmid

#163322

Purpose

eCas9 ABL1 gRNA 1 (GGGGGACACACCATAGACAG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 1_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
eSpCas9(1.1)-ABL1-gRNA2-exon4

Plasmid

#163323

Purpose

eCas9 ABL1 gRNA 2 (GAAGAAATACAGCCTGACGG), targeting exon 4 within the protein kinase domain of both ABL1 isoforms 1a and 1b.

Depositor

Val Brunton

Article

Beetham et al Cancer Res. 2021 Dec 17. pii: 0008-5472.

Insert

ABL1 gRNA 2_Exon4

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only
pGG431

Plasmid

#165615

Purpose

Vector for expression of SpCas9 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-CYC1t (KanR cassette in place of the PID)

Depositor

Marcus Noyes

Article

Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

Insert

TEF1p-SpCas9::KanR(in place of PID)-1xNLS-CYC1t (S. cerevisiae TEF promoter driving SpCas9 with KanR cassette replacing PID)

Use

CRISPR

Tags

SV40 NLS

Expression

Yeast

Mutation

Corrected homology relative to wild type SpCas9 (…

Promoter

TEF1

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj1b-IRES-EGFP

Plasmid

#164946

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFP

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj1b (kcnj1b Zebrafish)

Use

Tol2 transposon destination vector

Tags

IRES-EGFP

Promoter

actinb (actb2) promoter

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

Plasmid

#160609

Purpose

TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2-VP64:Tnos

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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hKCNB1 R312H

Plasmid

#124488

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed arginine 312 to histidine

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV29

Plasmid

#123401

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV29 (TRAV29DV5 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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hKCNB1 G379R

Plasmid

#124489

Purpose

missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

Depositor

Federico Sesti

Article

Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

Insert

human KCNB1 (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed glycine at 379 to arginine

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti C-MYC-DDK Puro PNRC1-W300A

Plasmid

#123301

Purpose

Mammalian expression of PNRC1-W300A mutant C-MYC-FLAG

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Lentiviral

Tags

MYC-FLAG

Expression

Mammalian

Mutation

W300A

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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DLG3_PDZ_2

Plasmid

#103947

Purpose

Protein expression and purification of NE-DLG PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG3 (DLG3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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