We narrowed to 12,753 results for: GAN

1. Flag-SKP1-RR (K142R/K163R)

   Plasmid

   #213425

   Purpose

   Expresses human SKP1 K142R/K163R mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-K142R/K163R (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-SKP1-F139A/I141A

   Plasmid

   #213687

   Purpose

   Expresses human SKP1-F139A/I141A mutant in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

   Insert

   human Flag tagged SKP1-F139A/I141A (SKP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACE-Truncated-Kir3.2-StreptagII

   Plasmid

   #177263

   Purpose

   Expresses a truncated from of human Kir3.2 with a C-terminal streptagII in insect cells

   Depositor

   Arthur Laganowsky

   Article

   Qiao et al J Phys Chem Lett. 2021 Dec 30;12(51):122

   Insert

   G-protein-gated inward rectifying potassium channel 2 (KCNJ6 Human)
   

   Use

   Tags

   StreptagII

   Expression

   Insect

   Mutation

   Residues 49-378

   Promoter

   polyhedrin promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGBacmam-NGS Wnt-Fc

   Plasmid

   #159629

   Purpose

   Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

   Depositor

   Chris Garcia

   Article

   Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

   Insert

   Fzd7/8 subtype NGS Wnt Fc
   

   Use

   Tags

   Fc tag and His tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3-EF1a-YapS127A

   Plasmid

   #46049

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes-kinase associated protein (YAP1 Human)
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S127A

   Promoter

   EF1a

   Available since

   July 2, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pETDuet_huCAD_ICADL (TevS)

   Plasmid

   #100098

   Purpose

   dual expression of human CAD and ICAD. The two caspase cleavage sites in ICAD were mutated to TEVP cleavable sequences

   Depositor

   Jim Wells

   Article

   Morgan et al Proc Natl Acad Sci U S A. 2015 Jul 7;112

   Insert

   DNA fragmentation factor subunit alpha (CAD, DFFA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   two caspase cleavage sites (DETD-117 and DAVD-224…

   Promoter

   T7

   Available since

   Jan. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3-EF1aH Yap S127A

   Plasmid

   #86497

   Purpose

   Mammalian expression of FLAG-tagged Yap containing S127A mutation

   Depositor

   Xin Chen

   Article

   Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

   Insert

   Yes associated protein 1 (YAP1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S127A

   Promoter

   EF1a

   Available since

   Feb. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)

   Plasmid

   #221329

   Purpose

   Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

   Insert

   FLAG-IRSp53 and HA-Cdc42(G12V) (Baiap2, Cdc42 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   G12V

   Promoter

   TRE3G

   Available since

   July 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1_c-SRC

   Plasmid

   #171850

   Purpose

   pEGFP-N1_c-SRC

   Depositor

   Michele Pagano

   Article

   Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

   Insert

   c-SRC (SRC Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Added a linker sequence in 3' of c-SRC: GSGS

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pML333_LAMP1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227898

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    LAMP1 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pML334_RAB7A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227899

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB7A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227890

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227907

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-FLAG_FBXL7 (R310H)

    Plasmid

    #171849

    Purpose

    pcDNA3-FLAG_FBXL7 (R310H)

    Depositor

    Michele Pagano

    Article

    Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

    Insert

    F-box and leucine rich repeat protein 7 (FBXL7 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed Arginine 310 to Histidine

    Promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR1A-Yap S127A

    Plasmid

    #46050

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Entry vector

    Tags

    2X Flag tag

    Expression

    Mutation

    S127A

    Promoter

    Available since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pCDNA3 ATP5O(5’UTR)-SL-FF

    Plasmid

    #85491

    Purpose

    Firefly luciferase under the control of ATP5O 5'UTR followed by a stem-loop

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    5'UTR of ATP5O followed by stem loop (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3 ATP5O(TISU)-FF

    Plasmid

    #85489

    Purpose

    Firefly luciferase under the control of ATP5O TISU

    Depositor

    Ivan Topisirovic

    Article

    Gandin et al Genome Res. 2016 May;26(5):636-48. doi:

    Insert

    Full TISU element of ATP5O (ATP5PO Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    2nd and 3rd codon of luciferase were modified fro…

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-FLEX-CAG_G-PTEN

    Plasmid

    #227438

    Purpose

    An AAV backbone for expression of the G-PTEN sensor under the CAG promoter, in a Cre dependent manner.

    Depositor

    Tal Laviv

    Article

    Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

    Insert

    G-PTEN sensor (Pten Rat)
    

    Use

    AAV

    Tags

    CD-sREACh and mEGFP

    Expression

    Mammalian

    Mutation

    R14G

    Promoter

    pCAG

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pML625_RTN4-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227895

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RTN4 homology arms with mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML624_RAB1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid

    #227906

    Purpose

    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

    Depositor

    Manuel Leonetti

    Article

    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

    Insert

    RAB1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge