We narrowed to 88,257 results for: Mal

1. MLF2-GFP-3xFLAG

   Plasmid

   #230986

   Purpose

   Expresses GFP tagged MLF2 in mammalian cells

   Depositor

   Christian Schlieker

   Article

   Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

   Insert

   MLF2 (MLF2 Human)
   

   Use

   Tags

   3xFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

   Plasmid

   #174899

   Purpose

   Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SaCas9v2-Puro

   Plasmid

   #178802

   Purpose

   SaCas9 with 2A-Puro, and a cloning backbone for sgRNA.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh (Cas9-2A Puro) and U6 (gRNA)

   Available Since

   Aug. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HCMVgO-sfGFP (TB40/E)

   Plasmid

   #136451

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) fused to superfolder GFP

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Use

   Tags

   Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-HCMVgH-8his (Merlin)

   Plasmid

   #136447

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tag

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein H (UL75 Human betaherpesvirus 5)
   

   Use

   Tags

   Short GGSG linker and 8his tag

   Expression

   Mammalian

   Mutation

   Extracellular domain (a.a. 1-716). Codon-optimize…

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-His:CRY2:nls:VPR

   Plasmid

   #135989

   Purpose

   Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

   Depositor

   Roman Jerala

   Article

   Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

   Inserts

   CRY2 domain fused to the VPR activation domain
   CRY2 domain fused to the VPR activation domain
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LUC05 (L/Cys/P)

   Plasmid

   #112146

   Purpose

   Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

   Depositor

   Paul Copeland

   Article

   Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

   Insert

   Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Rat, Photinus pyralis)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Available Since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRT/TO-Strep/HA-UBE2J1

   Plasmid

   #124669

   Purpose

   Expresses UBE2J1 with N-terminal Strep-HA tag in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

   Insert

   UBE2J1 (UBE2J1 Human)
   

   Use

   Tags

   2xStrep/HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PD-1-miSFIT-6-C, 16-C

   Plasmid

   #124681

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax

    Plasmid

    #173871

    Purpose

    Mammalian expression of mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax (opto-Rac1)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-Tiam-DHPH-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJZC116

    Plasmid

    #62344

    Purpose

    sgRNA + 2x MS2(wt+f6) with MCP-VP64 effector for mammalian cells, marked by BFP

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2x MS2 (wt+f6) binding module
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets CXCR4 , sequence: GCAGACGCGAGGAAGGAGGGCGC

    Promoter

    CMV and U6

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA_B2AR-s-pep

    Plasmid

    #47438

    Purpose

    FRET sensor for beta2-adrenergic receptor

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

    Insert

    beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pmiRFP2-Actin-C-18

    Plasmid

    #178965

    Purpose

    Expresses the fusion protein of miRFP2-Actin-C in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    miRFP2-actin
    

    Use

    Tags

    actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRLuc-CoV2-delStem1

    Plasmid

    #177616

    Purpose

    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    3-nucleotide (UAC) deletion in Stem 1

    Promoter

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT2 Beta1b GFP

    Plasmid

    #89893

    Purpose

    Mammalian expression of Beta1b GFP

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

    Insert

    Cacnb1 (Cacnb1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    GFP sequence inserted just before the Stop codon

    Promoter

    adenovirus major late promoter

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMT2 Beta1b mCherry

    Plasmid

    #89892

    Purpose

    Mammalian expression of Beta1b mCherry

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2016 Sep 23;291(39):20402-1

    Insert

    Cacnb1 (Cacnb1 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mCherry sequence inserted just before the Stop co…

    Promoter

    adenovirus major late promoter

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1-IFT43

    Plasmid

    #218735

    Purpose

    Expresses N-terminally EGFP-tagged IFT43 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Ishida et al Hum Mol Genet. 2021 Apr 26;30(3-4):213-2

    Insert

    IFT43 (IFT43 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_FOXA3_P2A_Hygro_Barcode

    Plasmid

    #120440

    Purpose

    Barcoded lentiviral vector to express FOXA3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    FOXA3 (FOXA3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Circular 200,100 with interspersed loops (FANCC)

    Plasmid

    #180192

    Purpose

    AAV vector carrying a guide RNA targeting the human FANCC mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Circular 200,100 with interspersed loops (CTNNB1)

    Plasmid

    #180193

    Purpose

    AAV vector carrying a guide RNA targeting the human CTNNB1 mRNA

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Insert

    Circular 200,100 guide RNA with interspersed loops
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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