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pGWB515_35S-HA-(ΔTP)DM3(Col-0) T359A

Plasmid

#229727

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, T359A

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Hh-0)

Plasmid

#229728

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Hh-0) ΔC

Plasmid

#229730

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Hh-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-(ΔTP)DM3(Col-0) ΔC

Plasmid

#229731

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB512_35S-FLAG-DM3(Col-0)68-510

Plasmid

#229733

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-67 were deleted, 511-515 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB515_35S-HA-(ΔTP)DM3(Col-0) Y460E V484E

Plasmid

#229715

Purpose

Stable transformation or transient expression of gene of interest in plants

Depositor

Eunyoung Chae

Article

Wan et al Molecular Cell, Volume 85, Issue 14

Insert

DM3(Col-0) (AT3G61540 Mustard Weed)

Expression

Plant

Mutation

amino acid 1-52 were deleted

Available Since

July 28, 2025

Availability

Academic Institutions and Nonprofits only

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FHpHG_mTent5c_LxxAA (L3m)

Plasmid

#221441

Purpose

Transfection into the cells for the reporter or qPCR assay.

Depositor

Marcos Morgan

Article

Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

Insert

Tent5c_LXXAA (L3m) (Fam46c Mouse)

Tags

FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

Expression

Mammalian

Mutation

L332A, LL335-336AA (CTC atc tct CTC CTG to CTC at…

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-DIO-mPOMC

Plasmid

#239042

Purpose

Overexpression of POMC gene

Depositor

Qingchun Tong

Article

Xu et al Cell Rep. 2023 May 30;42(5):112502. doi:

Insert

mPOMC (Pomc Mouse)

Use

AAV

Promoter

CAG

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGM63

Plasmid

#220169

Purpose

CRISRPi guide RNA against TOMM70A

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM70A (sgRNA) (TOMM70 Human)

Use

Lentiviral

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-MKI-MUT-P2A-GFP

Plasmid

#229406

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MKI-MUT-P2A-GFP

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

interaction mutant L109G; L143G (Control mutant)

Promoter

EFS

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAG155

Plasmid

#226744

Purpose

CRISPRi guide RNA against TOMM20

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM20 (TOMM20 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAG156

Plasmid

#226745

Purpose

CRISPRi guide RNA against TOMM20

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM20 (TOMM20 Human)

Use

Lentiviral

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2_ApyOAHIDS

Plasmid

#226246

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApyS

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO
ApyH
ApyI
ApyS

Tags

Hexa-Histidine

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSCrhaB2_ApyOAHID

Plasmid

#226245

Purpose

Production of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI

Depositor

Wilfred van der Donk

Article

Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

Inserts

His6_ApyA
ApyD
ApyO
ApyH
ApyI

Tags

Hexa-Histidine

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSuper DGK alfa mouse

Plasmid

#224573

Purpose

Negative Control for expression downregulation of DGK alfa human in human cells

Depositor

Isabel Mérida

Article

Avila-Flores et al Immunol Cell Biol. 2017 Jul;95(6):549-56

Insert

sh RNAi Diacylglycerol kinase alfa mouse (Dgka Mouse)

Use

RNAi

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGM295

Plasmid

#220183

Purpose

Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

SEC61B (SEC61B Human)

Use

Lentiviral

Tags

sfGFP

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv_V50A

Plasmid

#183734

Purpose

Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Mutation

V50A mutation in MFalpha signal

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-DNAJC5-ΔC30 (Δ169-198)

Plasmid

#205722

Purpose

Mammalian expression of DNAJC5-ΔC30 (Δ169-198)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Expression

Mammalian

Mutation

Δ169-198

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-XPACK-DNAJC5 (L115R)

Plasmid

#205717

Purpose

Mammalian expression of XPACK-DNAJC5 (L115R)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Tags

XPACK (GCINSKRKD)

Expression

Mammalian

Mutation

L115R

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-XPACK-DNAJC5 (WT)

Plasmid

#205716

Purpose

Mammalian expression of XPACK-DNAJC5 (WT)

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Tags

XPACK (GCINSKRKD)

Expression

Mammalian

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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